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Quick puterfaction bacteria agent for organic waste and constracture method thereof

A technology of organic waste and construction method, applied in the field of organic waste treatment, can solve the problems of narrow practical application range, unstable degradation function, poor anti-pollution ability, etc., and achieve good synergy, good degradation effect, and strong anti-pollution effect of ability

Inactive Publication Date: 2005-01-05
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 3. In the actual production and application process of bacterial agents made of pure strains, there are many problems such as poor anti-pollution ability, strict requirements on fermentation conditions, unstable degradation function, narrow practical application range, and high production costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Enrichment of lignocellulolytic bacteria under natural conditions by making high-temperature composting methods:

[0039] 1. Production of high temperature compost:

[0040] Weigh 500kg of fresh crop straw (such as rice straw, wheat straw, etc.), add 50kg of fresh livestock and poultry manure (chicken manure, pig manure, cow manure, etc.), adjust the moisture content to 65%, mix evenly, and pile into a mountain-shaped pile body. In the composting process, turn the compost every 2 days when the temperature is raised to high temperature, and adjust the moisture content at the same time, so that the temperature of the compost body should be controlled within 65°C as much as possible, and the moisture content should be kept at 60% to 65%. fermentation.

[0041] 2. Screening of high-temperature composite bacteria

[0042] A. Sampling: Sampling from compost samples in the high temperature stage;

[0043]B. Preparation of soil leaching solution: Add 200g of decomposed comp...

Embodiment 2

[0054] 1. Screening of high-temperature complex bacteria

[0055] A, sampling: sampling from high temperature stage compost sample in embodiment 1;

[0056] B. Preparation of soil leaching solution: Add 200g of decomposed compost or fertile vegetable garden soil to 1000mL of tap water, boil for 1 hour, filter, sterilize, and store for later use;

[0057] C, the preparation of liquid fermentation medium A (W / %): peptone 0.5, fresh rice straw powder 1, NaCl 0.5, CaCO 3 0.2, yeast powder 0.1, soil extract 10, 0.5×6cm Xinhua filter paper strips;

[0058] D. Screening of high-temperature composite bacteria: Weigh 5 g of the compost sample at the above-mentioned high-temperature stage, add 100 mL of sterile water into a 250-ml Erlenmeyer flask, and shake at 200 rpm for 30 minutes. Add it into test tube liquid fermentation medium A (1.8×18cm) at a ratio of 5% (v / v), keep the height of the medium at about 6-10cm, and carry out static enrichment culture at 60°C. When the filter pap...

Embodiment 3

[0067] 1. Screening of high-temperature complex bacteria

[0068] A, sampling: take a sample from the high temperature stage compost sample described in embodiment 1;

[0069] B. Preparation of soil leaching solution: Add 200g of decomposed compost or fertile vegetable garden soil to 1000mL of tap water, boil for 1 hour, filter, sterilize, and store for later use;

[0070] C, the preparation of liquid fermentation medium A (W / %): peptone 0.5, fresh rice straw powder 1, NaCl 0.5, CaCO 3 0.2, yeast powder 0.1, soil extract 10, 0.5×6cm Xinhua filter paper strips;

[0071] D. Screening of high-temperature complex bacteria: Weigh 5 g of the compost samples at the above high-temperature stage, add 100 mL of sterile water into a 250-ml Erlenmeyer flask, and shake at 150 rpm for 30 minutes. Add it into test tube liquid fermentation medium A (1.8×18cm) at a ratio of 5% (v / v), keep the height of the medium at about 6-10cm, and carry out static enrichment culture at 50°C. When the fi...

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Abstract

The invention is an organic waste fast-decaying bacterium agent and its constructing method, including different kinds of microbes: bacteria, actinomyces, epiphytes, etc, and different microbes have good cooperative effect. It can be obtained by directly separating and screening lignocellulose-degrading bacteria in the form of compound bacteria. It has not only high-efficacy stable degrading function to organic wastes like stalks, dejecta of domestic animals fowls, life rubbishes, etc but also strong adaptability to different conditions and antipollution ability, and has wide application prospect in regenerating and reusing organic wastes resources, environmental protection, etc.

Description

technical field [0001] The invention relates to a fast-degrading composite bacterial agent for organic waste and a construction method thereof, belonging to the technical field of organic waste treatment. Background technique [0002] With the development of social economy, more and more organic wastes are produced in various fields of society. Whether it is a developed country or a developing country, it is inevitable to face the serious threat of waste disasters. Organic waste has become a major social hazard. It pollutes the environment, devours cultivated land, and endangers people's physical and mental health. How to make organic waste harmless, recycle, reduce, and turn waste into treasure has become a worldwide problem that needs to be solved urgently. Biodegradation is currently one of the effective ways to recycle and utilize organic waste, which is generally recognized. It has broad application prospects in the production of organic fertilizer, feed, sugar, fuel ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F9/04C05F11/00C12N1/14C12N1/20C12N1/22C12S3/04
CPCY02A40/20
Inventor 朴哲杨林章殷士学胡健
Owner YANGZHOU UNIV
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