Process for preparing collagen active polypeptide from avian animal tissue
A technology of active peptides and animal tissues, applied in the direction of connective tissue peptides, animal/human proteins, specific peptides, etc., can solve the problems of long reaction time, strict control conditions, loss of activity, etc., achieve high amino acid content, simple and rapid process , Increase the effect of water solubility
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example 1
[0032] Take 10000 g of duck feet (duck toes, duck webs and duck foot stems), clean them, soak in 5.0% acetic acid solution for 400 hours, wash with pH 6.0, and soak in deionized water for 24 hours. Then, it was extracted in hot water at 72°C for 8 hours, filtered, and the obtained filtrate was concentrated under reduced pressure at 50°C and 0.099MPa, so that the collagen content in the concentrated solution was 5% (w / w), and added to the concentrated solution Adjust the pH to 3.5 with citric acid. Hydrolyze at 90°C for 3 hours, adjust the pH value to 7.0 with sodium carbonate, let it stand for 2 hours, then centrifuge at 8000rpm for 20min, discard the precipitate, and obtain an aqueous collagen polypeptide solution. Add organic solvent mixture (acetone and methanol 1:1, v / v) to it, and the ratio of collagen polypeptide aqueous solution is 4:1 (v / v), centrifuge 20min under 8000rpm, discard supernatant to obtain precipitation, will precipitate in Dry at 65° C. for 30 hours to o...
example 2
[0034] Take 2000 g of duck foot stems, clean them, soak them in 3.0% citric acid solution for 480 hours, wash with water at a pH of 5.0, and soak them in deionized water for 16 hours. Then, it was extracted in hot water at 85°C for 4 hours, filtered, and the obtained filtrate was concentrated under reduced pressure at 55°C and 0.099MPa, so that the collagen content in the concentrated solution was 15% (w / w), and added to the concentrated solution Sulfuric acid was used to adjust the pH to 4.0. Hydrolyze at 68°C for 7 hours, adjust the pH value to 7.5 with potassium hydroxide, let it stand for 8 hours, then centrifuge at 8000rpm for 15min, discard the precipitate, and obtain collagen polypeptide aqueous solution. Add organic solvent mixture (acetone and ethanol 2: 1, v / v) to it, and the ratio of collagen polypeptide aqueous solution is 4.5: 1 (v / v), centrifuge 15min under 8000rpm, discard supernatant to obtain precipitation, will precipitate in Dry at 50° C. for 30 hours to ob...
example 3
[0036]Take 750g of duck feet, clean them, soak in 1.0% sulfuric acid solution for 450 hours, wash with pH value 4.0, and soak in deionized water for 18 hours. Then, it was extracted in hot water at 65°C for 8 hours, filtered, and the obtained filtrate was concentrated under reduced pressure at 65°C and 0.097MPa, so that the collagen content in the concentrated solution was 25% (w / w), and added to the concentrated solution Mixture of acetic acid and hydrochloric acid (acetic acid and concentrated hydrochloric acid 6:1, v / v), adjust the pH to 0.5. Hydrolyze at 65°C for 8 hours, adjust the pH value to 7.0 with sodium bicarbonate, let it stand for 8 hours, then centrifuge at 7000rpm for 18min, discard the precipitate, and obtain an aqueous collagen polypeptide solution. Add organic solvent mixture (ethanol and isopropanol 4:1, v / v) to it, and the ratio of collagen polypeptide aqueous solution is 3:1 (v / v), centrifuge 18min under 7000rpm, discard supernatant to obtain precipitation...
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