Soya sauce containing organic selenium from aspergillus oryzae and preparation method thereof
The technology of Aspergillus oryzae and organic selenium is applied to the field of soy sauce containing organic selenium derived from Aspergillus oryzae and the production field thereof. The effect of simplifying the preparation process and preventing diseases
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Embodiment 1
[0030]Weigh 7 parts of soybeans, 2 parts of broken rice, and 2 parts of rice bran, crush them and mix them evenly. Take 100 kg of mixed koji, steam and cool them, use them as koji, soak them for later use. 2.19g of sodium selenite was dissolved with 100ml of physiological saline, and the insoluble components were removed by filtration to obtain a 10g / L selenium mother liquor. Take 0.15L of selenium mother liquor, dilute it with water soaked in koji, add it to 100kg of koji, mix and stir evenly. Make koji for 24 hours at 25°C. Crush the koji, transfer it to a fermentation tank, stir continuously, and ferment and cultivate at 37°C for 70 hours to make moromi. Add 30% brine to the soy mash until the NaCl concentration in the soy mash is 3%, and stir at 50°C for 24 hours to autolyze the Aspergillus oryzae and release nutrients such as organic selenium, protein, amino acid, and nucleic acid biosynthesized in the cell. Add physiological saline or distilled water, and drip to obtai...
Embodiment 2
[0032] Weigh 6 parts of soybeans, 2 parts of starch, and 3 parts of bran, grind them and mix them evenly. Take 100 kg of mixed koji, steam and cool them, use them as koji, soak them for later use. 0.219g of sodium selenite was dissolved with 1000ml of physiological saline, and the insoluble components were removed by filtration. Take 0.3L of selenium mother liquor, dilute it with water soaked in koji, add it to 100kg of koji, mix and stir evenly. Make koji for 28 hours at 23°C. Crush the koji, transfer it to a fermentation tank, stir continuously, and ferment and cultivate at 35°C for 65 hours to make moromi. Add 10% brine to the soy mash until the NaCl concentration in the soy mash is 1.5%, and stir at 20°C for 40 hours to autolyse the Aspergillus oryzae and release nutrients such as organic selenium, protein, amino acid, and nucleic acid biosynthesized in the cell. Add physiological saline or distilled water, and drip to obtain the first oil and the second oil, so that the...
Embodiment 3
[0034] Weigh 5 parts of soybeans, 1 part of starch, and 2 parts of bran, grind them and mix them evenly, take 100 kg of mixed koji, steam and cool them, use them as koji, soak them for later use. 21.88 g of sodium selenite was dissolved in 100 ml of physiological saline, and the insoluble components were removed by filtration. Take 0.03L of selenium mother liquor, dilute it with water soaked in koji, add it to 100kg of koji, mix and stir evenly. Make koji at 25°C for 2 hours. Crush the koji, transfer it to a fermentation tank, stir continuously, ferment and cultivate at 38°C for 40 hours, and make moromi. Add 10% brine to the soy mash until the NaCl concentration in the soy mash is 5%, and stir at 80°C for 5 hours to autolyze the Aspergillus oryzae cells and release nutrients such as organic selenium, protein, amino acid, and nucleic acid biosynthesized in the cells. Add physiological saline or distilled water, and drip to obtain the first oil and the second oil, so that the...
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