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Hydroxy alkanoic acid polymer and its producing method

A technology of hydroxyalkanoic acid and production method, which can be applied to bacteria, fermentation and other directions, can solve the problems of PHA increasing the difficulty of product extraction, high price, and not using recombinant bacteria.

Inactive Publication Date: 2005-08-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, genetically recombinant bacteria are generally not used as production strains in the production process of food and medical products to avoid biosafety problems
(4) In the relevant research on hydroxyalkanoic acid polymers similar to the present invention, the main raw materials used are olive oil and oleic acid, soybean oil and lauric acid, sodium gluconate and lauric acid, etc., and no downstream extraction has been reported. and processing
Generally speaking, using oil as a carbon source to ferment and produce PHA will increase the difficulty of product extraction and often affect the quality of PHA
However, using sodium gluconate as a fermentation raw material will increase production costs and affect its application range due to its high price.

Method used

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  • Hydroxy alkanoic acid polymer and its producing method
  • Hydroxy alkanoic acid polymer and its producing method
  • Hydroxy alkanoic acid polymer and its producing method

Examples

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example 1

[0072] Example 1 Fermentative production of P (HBHO) synthesized with glucose as carbon source

[0073] (1) Inclined plane and shake flask seed culture

[0074] Inoculate the preserved S. fredii strain on the slant medium, activate it twice in an incubator at 30°C, transfer it to a shake flask containing a shake flask seed medium, and activate it twice again, and wait for the next stage of shaking Flask cultured for 14 hours, when the bacterial cell concentration reached 1.53g stem cells / L (OD 600 When the value is 3.0), the cell growth is still in the logarithmic growth phase, which is used as the seed liquid of the seed tank for subsequent use.

[0075] (2) Seed tank seed culture

[0076] Take a 10L self-control tank as the seed tank, use fermented seed culture medium, the filling coefficient is 0.7, insert the seed solution, the inoculum amount is 8%, cultivate at 30°C, adjust and control the pH to 7.0-7.2 through 8mol NaOH solution, and keep the solution When the oxygen...

Embodiment 2

[0084] Example 2 Fermentative production of P(HBHO) synthesized by using starch hydrolyzate as carbon source

[0085] (1) Inclined plane and shake flask seed culture

[0086] Inoculate the preserved Sfredii strain on the slant medium, activate it twice in the incubator at 28°C, transfer it to the shake flask containing the shake flask seed medium, and activate it twice again, and cultivate it in the next stage of shake flask 16h, when the bacterial cell concentration reached 2.3g stem cells / L (OD 600 When the value is 4.5), the cell growth is still in the logarithmic growth phase, and it is used as the seed liquid of the seed tank for subsequent use.

[0087] (2) Seed tank seed culture

[0088] A 10L self-control tank is used as the seed tank, a fermented seed medium is used, the filling coefficient is 0.7, the seed solution is inserted, the inoculation amount is 8%, and the culture is carried out at 28°C, and the pH is adjusted and controlled by 8 mol of NaOH solution to ma...

Embodiment 3

[0094] Embodiment 3, use waste molasses as the fermentative production of carbon source synthesis P (HBHO)

[0095] (1) Inclined plane and shake flask seed culture

[0096] Inoculate the preserved S. fredii strain on the slant medium, activate it twice in an incubator at 32°C, transfer it to a shake flask containing a shake flask seed medium, and activate it twice again, and wait for the next stage of shaking Flask cultured for 18h, when the bacterial cell concentration reached 2.85g stem cells / L (OD 600 When the value is 5.5), the cell growth is still in the logarithmic growth phase, and it is used as the seed liquid of the seed tank for subsequent use.

[0097] (2) Seed tank seed culture

[0098] Use a 10L self-control tank as the seed tank, use a fermented seed medium with a loading coefficient of 0.7, insert the seed liquid, and inoculate the amount of 10%, cultivate at 32°C, adjust and control the pH to 7.0-7.2 through 8mol NaOH solution, and maintain the solution. Whe...

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Abstract

The present invention discloses one kind of hydroxy alkanoic acid polymer, that is, 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer (PHBHO). The 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer (PHBHO) is prepared with Sinorhizobium fredii as production strain, carbohydrate as carbon source and ammonium salt as nitrogen source, and through high density fermentation and metabolism regulation with lauric acid in optimal culture medium and under optimal culture condition. Corresponding fermentation management method, separation and purification method and quality detection method are established. The present invention can obtain PHBHO product up to 14.4-22.1 g / L, of molecular weight of 112000-185000 Da, and endotoxin content 4-6 EU / g, and the novel nanometer level material may be used in biomedicine and medical tissue engineering.

Description

technical field [0001] The invention relates to a polymer compound obtained by forming a carboxylate bond on a polymer main chain, and specifically belongs to a hydroxyalkanoic acid polymer (hereinafter referred to as PHA) and a production method thereof. Background technique [0002] PHA is a kind of high molecular polymer with simple structure biosynthesized, and it is the main carbon source and energy storage material of prokaryotes. Under the condition of unbalanced metabolism, prokaryotes will use the remaining nutrients to synthesize PHA and distribute it discretely in the cells in the form of particles, and its content can reach up to 90% of the dry weight of the cells. Decomposition and utilization. It is precisely because of this biological characteristic that endows this kind of material with biorenewability and biodegradability. [0003] Studies have shown that PHA has very similar physical and chemical properties to general-purpose plastics, such as polypropyle...

Claims

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Application Information

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IPC IPC(8): C08G63/06C12N1/20C12P7/42
Inventor 赵良启冯涛肖婧凡王海宾
Owner SHANXI UNIV
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