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Process for preparing high active powder composite green storage inoculating agent and its carrier adsorption agent

A technology of inoculant and powder, which is applied in the field of preparation of highly active powder compound silage inoculant and its carrier adsorption, can solve the problems of large loss of nutrients and easy corruption, and achieve the effects of promoting fermentation, improving quality, and expanding feed resources

Inactive Publication Date: 2005-08-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional silage technology loses a lot of nutrients during the silage process, and it is easy to rot after opening the cellar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of high activity powder compound silage inoculant

[0029] The fermentation strain uses Streptococcus lactis CVCC590 as the initial fermentation strain, and Lactobacillus plantarum AS1.557 as the subsequent fermentation strain.

[0030] After seed cultivation, the seed fermentation broth is inoculated into an industrial production fermentor. The medium composition is: 0.5-5% of yeast, 3-5% of rice glucose syrup, and mineral composition (content in 1 liter of medium) K 2 HPO 4 1-5g, diammonium citrate 1-6g, sodium acetate 2-8g, MgSO 4 ·7H 2 O 0.3-1g, MnSO 4 ·4H 2 O 0.1-1g.

[0031] Culture condition

[0032] Seed culture: Using simultaneous inoculation and asynchronous fermentation technology, the Streptococcus lactis and Lactobacillus plantarum are simultaneously connected to a 10 liter seed tank for cultivation. The seed culture medium is used. The inoculum of each strain is 4% of the inoculum and cultivated at 40℃ Incubate for 18 hours;

[0033] Industrial pro...

Embodiment 2

[0040] Preparation of high activity powder compound silage inoculant

[0041]The fermentation strain uses Streptococcus lactis CVCC590 as the initial fermentation strain, and Lactobacillus lactis AS1.9 as the subsequent fermentation strain.

[0042] After seed cultivation, the seed fermentation broth is inoculated into an industrial production fermentor. The medium composition is: yeast 0.5-5%, 3-8% corn glucose syrup, the remaining mass fraction is divided into water, and the mineral composition ( Content in 1 liter of medium) K 2 HPO 4 1-5g, diammonium citrate 1-6g, sodium acetate 2-8g, MgSO 4 ·7H 2 O 0.3-1g, MnSO 4 ·4H 2 O 0.1-1g.

[0043] Culture condition

[0044] Seed culture: Using simultaneous inoculation and asynchronous fermentation technology, the Streptococcus lactis and Lactobacillus are simultaneously connected to a 10 liter seed tank for cultivation. The seed culture medium is used. The inoculum of each strain is 8% of the inoculum, 35°C incubator Cultivate for 18 h...

Embodiment 3

[0052] Using the above-mentioned medium in this specification, inoculate Streptococcus lactis and Lactobacillus into the seed medium, prepare seeds in a 10-liter fermenter, and then connect to a 150-liter fermenter for fermentation. The fermentation broth is subjected to continuous high-speed centrifugation to obtain bacterial sludge, and then the protective agent, cellulase and carrier adsorbent are added according to the method of this specification, and the product is prepared into powder through a 18-25 mesh sieve. Pack and seal with aluminum foil and store at 0-4℃.

[0053] The silage inoculant produced by the method is directly and evenly sprinkled into the silage corn stalks at an added amount of 0.5 kg / ton.

[0054] Table 1 Chemical composition analysis of 60-day silage corn (g / kg dry matter)

[0055] Item Control group Test group

[0056] Dry matter 215.5 220.7

[0057] Soluble carbohydrates 40.19 34.00

[0058] Neutral detergent fiber 667.8 630.7

[0059] Acid deterge...

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PUM

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Abstract

The present invention belongs to the field of silage fermenting promoter technology. The present invention includes simultaneous inoculation of and asynchronous with of lactostreptococcus as initial fermenting bacteria and lactobacillus as subsequent fermenting bacteria, normal temperature carrier adsorption post-processing technology, optimizing the recipes of seed culture medium activating factor, industrial fermenting culture medium and protecting agent, and re-compounding fermented lactic acid bacteria and cellulase. The present invention has the features of mild production condition, low cost, high product activity, etc. The product may fit different silage materials and different silage conditions and can promote the fermentation of lactic acid bacteria and the homotypic fermentation, control negative fermentation, reduce loss of nutrients during fermentation and make full use of resource.

Description

Technical field [0001] The invention relates to a high-activity powder composite silage inoculant and a preparation method for carrier adsorption thereof, belonging to the technical field of silage additives in the feed industry, and more specifically classified as silage fermentation promoters. technical background [0002] The situation of 400 kilograms of grain per capita per year in our country will not change for a long time, that is, our country cannot use more grain as feed grain. It is estimated that by 2000, the annual feed gap will reach 40 million tons. The use of crop straws, pastures, aquatic feed and other raw materials to prepare silage, develop feed resources, improve feed utilization, and develop food-saving grass-eating livestock more and more quickly and increase meat production have become my country's basic national policies. [0003] my country is a large agricultural country. It produces more than 500 million tons of various green roughage every year, of wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K30/18C12N1/20
Inventor 乐国伟李永富施用晖朱建津沈国强
Owner JIANGNAN UNIV
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