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Glucoside active site LWD-b in Liuwei Dihuang decoction, its preparation and medical usage

A technology of Liuwei Dihuang Decoction and active parts, which can be applied in medical formulas, diseases, anti-tumor drugs, etc., and can solve problems such as inconvenient medication, difficult drug dosage forms, and unclear active ingredients

Active Publication Date: 2005-09-07
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in the actual application of this prescription, no matter it is used as decoction, pill, capsule or other dosage forms, there are obvious deficiencies, which are mainly manifested in the following aspects: one is that the dosage is large, and it is inconvenient to use it, and it is difficult to make it into a modern convenient drug. The second is that the active ingredients are not clear, and the active ingredients are not used as quality control indicators, so it is difficult to ensure the stability and controllability of product quality; the third is that when various reasons lead to large changes in the content of active ingredients in medicinal materials, there is no effective means to ensure the stability of the content of active ingredients in the product, so that the effectiveness of clinical medication cannot be guaranteed

Method used

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  • Glucoside active site LWD-b in Liuwei Dihuang decoction, its preparation and medical usage
  • Glucoside active site LWD-b in Liuwei Dihuang decoction, its preparation and medical usage
  • Glucoside active site LWD-b in Liuwei Dihuang decoction, its preparation and medical usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Preparation of the glycoside active fraction LWD-b of Liuwei Dihuang Tang extract

[0063] The single herbs of Liuwei Dihuang Decoction are prepared according to the common ratio, such as Rehmannia glutinosa: Cornus officinalis: Chinese yam: Alisma: Alisma: Cortex paeonol: Poria cocos (8:4:4:3:3:3) or after adding or subtracting some of the herbs, 1500 grams of crude drug is added, boiled twice in boiling water with six times the weight of crude drug, each time for two hours, filtered with gauze (six layers) and absorbent cotton, concentrated under reduced pressure until the ratio of crude drug (weight) to extract (volume) is 1:1 .

[0064] Add ethanol to the extract with stirring to a final ethanol concentration of 30% (v / v), stir, and place overnight. Centrifuge (2500 rpm, 25min). The precipitate was washed 4 times with 30% ethanol, centrifuged, and the precipitate was discarded. The supernatants were combined and concentrated to 1000 ml, and the final e...

Embodiment 2

[0066] Embodiment 2: The biological activity experiment of the extract LWD-b of Liuwei Dihuang Decoction of embodiment 1

[0067] 1. The effect of LWD-b on immune function

[0068] 1. The effect of oral LWD-b on the proliferation response of splenocytes in normal mice

[0069] Female Balb / c mice were given LWD-b by intragastric administration, once a day, for 7 consecutive days. On the 8th day, the mice were executed by decapitation, the spleen was aseptically taken, and the spleen cell suspension was prepared, and the cell concentration was adjusted to 5×10 with complete RPMI-1640 culture medium. 6 Cells / ml, take 100μl Con A (final concentration is 0.5ug / ml) and 100μl cell suspension and add them into 96-well plate in turn, and replace Con A with 1640 culture medium in the control group, and place at 37°C, 5% CO 2 Incubate in an incubator for 56 hours, add 20 μl to each well 3 H-TdR (10μCi / ml), continue to culture for 72 hours, collect cells on glass fiber filter paper wit...

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Abstract

A Liuwei Dihuang decoction (Glucoside active site LWD-b) extract includes, by weight of extract, 12.5-15.3 weight % total glycosides (morroniside, loganin, paeoniflorin), wherein the Liuwei Dihuang decoction is prepared by rehmannia, dogberry, Chinese potato, rhizoma alismatis, cortex moutan and tuckahoe with a weight proportion of 8:4:4:3:3:3.

Description

technical field [0001] The present invention relates to the extract of Liuwei Dihuang Decoction (glycoside active part LWD-b), its preparation method, and its use in the treatment or auxiliary treatment of various diseases accompanied by kidney yin deficiency syndrome and immune dysfunction, including tumors, tumor chemistry or Side effects of radiation therapy, leukopenia, senile immune dysfunction, infection, etc.; autoimmune diseases such as systemic lupus erythematosus, nephrotic syndrome, nephritis, bronchitis and bronchial asthma, prostatitis, aplastic anemia, thrombocytopenia Immune dysfunction in patients with purpura, myasthenia gravis, and senile diseases; and diseases caused by reproductive and endocrine system dysfunction or disorders, including climacteric syndrome, ovarian dysfunction, infertility, habitual abortion, plant Nervous disorders, chloasma, gynecomastia, senile sexual decline, menopausal osteoporosis, senile osteoporosis, etc. technical background ...

Claims

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Application Information

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IPC IPC(8): A61P11/00A61P13/08A61P13/12A61P15/10A61P15/12A61P15/14A61P17/00A61P19/10A61P31/00A61P35/00A61P37/04
Inventor 赵毅民乔善义张永祥周文霞程军平赵修南董剑英孙磊杨胜马渊齐春会任凤霞熊善丽郭继芬
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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