The invention discloses a preparation method of Liuwei Dihuang pills. The Liuwei Dihuang pills comprise the following medicinal effective components in parts by weight: 160 parts of prepared radix rehmanniae, 80 parts of wine-prepared fructus corni, 60 parts of moutan bark, 80 parts of Chinese yam, 60 parts of poria cocos and 60 parts of rhizoma alismatis. The preparation method of the pills comprises the following steps: (1) preparing medicinal powder: taking the prepared radix rehmanniae, the fructus corni, the moutan bark, the Chinese yam, the poria cocos and the rhizoma alismatis, crushing, sieving and uniformly mixing to obtain the medicinal powder; (2) preparing an adhesive: taking glycerol into a container, adding medicinal carboxymethyl cellulose salt, uniformly stirring, sealing, and heating until the carboxymethyl cellulose salt is completely swelled, so as to prepare the adhesive for later use; and (3) preparing the pills: adding the adhesive accounting for 25-50% of the total amount of the prepared medicine into a paste powder mixture, uniformly mixing, and pelleting to obtain the Liuwei Dihuang pills. According to the invention, the prepared Liuwei Dihuang pills have the beneficial effects that ethanol, refined honey and water are not used as adhesives, the prepared Liuwei Dihuang pills do not need to be subjected to a drying process, the volatile component paeonol in the finished product is low in loss, the content is relatively high, the dispersion time limit is short, and the quality is stable and controllable.