Method for producing fodder protein by solid-solid state fermenting apple residues

A technology for solid-state fermentation and feed protein, applied in biochemical equipment and methods, fermentation, microorganisms, etc., can solve the problems of large amount of feed protein seed medium, high investment and production costs, and complicated liquid culture of strains, etc., to achieve convenient Popularization and application, low investment and production costs, rapid bacterial growth

Inactive Publication Date: 2005-09-28
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overcome the shortcomings of the existing single-solid-state fermentation production of feed protein seed medium, which requires a large amount of medium, complex liquid culture of strains, complex equipment, and high investment and production costs

Method used

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  • Method for producing fodder protein by solid-solid state fermenting apple residues

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Experimental program
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Embodiment 1

[0037] Embodiment 1: the production of feed protein

[0038] Seed medium: 200g potato (potato), 20g sucrose, some water; the production method is: cut the potato into small pieces, add appropriate amount of tap water to boil for 20-30min, filter, take the filtrate and add tap water to 1000mL, add 20g sucrose, dissolve and mix well.

[0039] Cultivation of seed strains: Take 300mL seed culture medium into a 500mL Erlenmeyer flask and sterilize at 121°C for 20 minutes, then insert the slant strains after cooling to room temperature, and cultivate in an air bath shaker at 30-32°C, 120-140rpm 36-54h to obtain mature seed strains.

[0040] The composition of solid-state fermentation medium A is: apple pomace 65%, bran 21%, cottonseed cake 10%, urea 2%, (NH 4 ) 2 SO 4 1%, KH 2 PO 4 1%, add water to adjust the initial water content to 60%.

[0041] The mixed strains and ratios of the inoculation: Candida utilis: Saccharomyces cerevisiae: Trichoderma viride=4:2:3.

[0042] Sol...

Embodiment 2

[0058] Embodiment 2: the production of thalline koji

[0059] The cultivation of seed bacterial classification is identical with embodiment 1.

[0060] Fresh and wet apple pomace was dried at 75°C in a hot-air drying oven and crushed through a 24-mesh sieve to make solid-state fermentation medium A (70% of apple pomace, 20% of bran, 5% of cottonseed cake, 2% of urea, (NH 4 ) 2 SO 4 1%, KH 2 PO 4 2%, add water to adjust the initial water content to 65%. ), then insert the mature mixed strains of seed culture (Candida utilis: Saccharomyces cerevisiae: Trichoderma viride=3.5: 1.5: 3) in the amount of 15mL / 100 substrates, at 32°C, after solid-state fermentation for 54h The solid-state fermented product was transferred to a quick drying box, and dried at 34°C for 30 hours to obtain apple pomace koji.

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Abstract

The solid-solid fermentation process of producing feed protein with apple residue includes the following steps: compounding solid fermentation culture medium A with the crushed apple residue and inoculating mature mixed strain for solid fermentation; fast low temperature drying the fermented product; and inoculating the dried product into solid fermentation culture medium B for the second solid fermentation to produce the apple residue feed protein product. Compared with single solid fermentation process, the solid-solid fermentation process of the present invention needs no large fermenting tank for liquid phase culture, and thus is simple, low in cost, convenient in transportation and less strain death.

Description

technical field [0001] The invention belongs to a method for producing bacterial koji and feed protein by fermenting apple pomace. Background technique [0002] Commonly referred to as feed protein (Single cell protein, SCP), also known as microbial protein or bacterial protein, refers to yeast, fungi, mold, and non-disease bacteria in single-celled organisms. It contains protein, the content is as high as 45%-74%, and various amino acids are reasonably matched, complete in variety, rich in vitamins and trace elements, and free of cholesterol. These advantages are unmatched by general crop feed. [0003] China is the world's largest apple producer, with an annual output of more than 22 million tons, more than one-third of the world's total. Shaanxi Province is also the largest apple production base in my country. At present, the province's total apple planting area reaches 6.02 million mu, with an annual total output of more than 5.5 million tons, ranking first in the count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12P21/00
Inventor 宋纪蓉徐抗震马海霞黄洁
Owner NORTHWEST UNIV(CN)
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