Medicinal composition using anterior pituitary adrenal cortical extract as main component, and its preparation method and use

A technology of the adrenal cortex and the anterior pituitary gland, applied in the field of medicine, can solve the problems of human immunosuppression, strengthening the ulcer-induced effect of glucocorticoids, and the negative feedback effect of the adrenal cortex

Inactive Publication Date: 2005-10-26
蔡海德
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the dose of glucocorticoids is large, it is easy to cause human immunosuppression, proteolysis and negative feedback to the adrenal cortex; aspirin and other non-steroidal anti-inflammatory drugs will strengthen the ulcer-causing effect of glucocorticoids
Although the existing pituitary anterior lobe adrenal cortex extract injection does not have the above-mentioned side effects, patients with painful injections will refuse to use it

Method used

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  • Medicinal composition using anterior pituitary adrenal cortical extract as main component, and its preparation method and use
  • Medicinal composition using anterior pituitary adrenal cortical extract as main component, and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Preparation of adrenocorticotropic hormone (ACTH):

[0092] (1) Raw material handling

[0093] Take out the qualified porcine pituitary gland and porcine adrenal cortex, quickly remove the connective tissue (fascia), quickly put it into analytical pure acetone, soak and dehydrate for 3 times, about 24 hours each time, until the pituitary gland and porcine adrenal cortex harden. Separate the adenohypophysis and neurohypophysis in the pituitary gland and store them in acetone for later use. This method uses the pituitary gland, takes out the pituitary gland and the adrenal cortex (the adrenal cortex accounts for 1 / 4-1 / 2 of the total weight of the two), puts it in room temperature for vacuum drying, grinds it into powder, and passes through an 80-mesh sieve to obtain the pituitary gland dry powder , the moisture content should be less than 5%.

[0094] (2) Extraction:

[0095] Add 1Kg of adenohypophysis dry powder (including 1 / 4-1 / 2 of the weight ratio of the adrenal co...

Embodiment 2

[0097] Preparation of thymosin:

[0098] Soak the frozen calf thymus (weight ratio 2:1) with purified water (deionized water), remove the connective tissue, membrane and residual meat after thawing, and wash it repeatedly with purified water 4 times, shake off the water, and use 10Kg of thymus with Mix 10Kg of water for injection and mince in a meat grinder, grind and homogenate in a colloid mill for 2-3 times at a temperature below 10°C until the particle size is about 1 μm, adjust the pH value of the ground homogenate with 50% citric acid 1.5-2.0, add 800g of pepsin, raise the temperature to 40-50°C, keep it warm for 20-30min, then rise to 75°C for 20min to inactivate the enzyme, then quickly cool down to room temperature, and put it in a 316 stainless steel bucket (with cover) Freeze at -28°C for 24 hours in a sterile room, take it out and slow down at room temperature for 8 hours, re-freeze at -28°C for 24 hours, then cool at room temperature for 8 hours, heat to 60-70°C, ...

Embodiment 3

[0101] Preparation of immune ribonucleic acid (iRNA):

[0102] Select one-year-old sheep, check for infectious diseases, Brucella and cysticercosis, add 2-3mg / ml dead or expired BCG with time-consuming adjuvant (1 part of lanolin, 1 part of liquid paraffin, mixed , autoclaved, stored at 4°C for later use) made into water-in-oil emulsifier, injected into the lymph nodes of sheep, each sheep was injected with 1ml, slaughtered after 12-14 days, and the liver, spleen, heart, lung and kidney were taken for extraction The raw material of iRNA was washed with normal saline, and was twisted into pieces with a meat grinder, and 1-2 times the volume of 0.1mol / L sodium chloride-0.05mol / L trisodium citrate, 0.001% polyvinyl sulfate ( PVS), 0.1% TritonX-100 (PH7), homogenized with a colloid mill 2-3 times, ground into particles of about 1 μm, centrifuged at 0-2°C, 3500 rpm for 20 minutes, and took the upper layer to clear overnight.

[0103] Add an equal volume of 0.2mol / L triethanolamine...

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Abstract

A composite medicine for treating arthritis and viral cold and preventing cancer and injection pain contains corticotropic hormone, thymopeptide and immune ribonucleic acid, which are extracted from anterior pituitary adrenal cortex, and the process for preparing its liquid (or freeze-dried powder) injection are disclosed.

Description

Technical field: [0001] The present invention relates to a kind of medicaments, especially relate to a kind of combination medicaments with pituitary anterior lobe adrenal cortex extract as main component and its preparation method, quality detection method and its preparation treatment rheumatoid arthritis, rheumatoid arthritis, Application in medicines for viral colds, hepatitis and other diseases. Background technique: [0002] Currently, the drugs used to treat rheumatoid arthritis and rheumatoid arthritis are non-steroidal anti-inflammatory drugs such as aspirin, glucocorticoids, and the like. However, when the dose of glucocorticoid is large, it is easy to cause human immunosuppression, proteolysis and negative feedback to the adrenal cortex; non-steroidal anti-inflammatory drugs such as aspirin will strengthen the ulcer-causing effect of glucocorticoid. Although the existing pituitary anterior lobe adrenal cortex extract injection does not have the above-mentioned si...

Claims

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Application Information

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IPC IPC(8): A61K38/35
Inventor 蔡海德
Owner 蔡海德
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