Luminous microsphere and its production method and water dispersion system

A technology of fluorescent microspheres and microspheres, which is applied in the direction of chemical instruments and methods, luminescent materials, etc., and can solve problems such as complex processes, complex preparation processes, and harsh preparation conditions

Active Publication Date: 2005-11-02
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the TOP / TOPO method has many advantages in the preparation of fluorescent nanoparticles, there are still many problems. The main reason is that the preparation process of the TOP / TOPO method is relatively complicated and the preparation conditions are harsh.
In addition, the obtained fluorescent nanoparticles are only soluble in organic solvents, which limits the use of the above-mentioned fluorescent particles to a certain extent.
If you want to convert the oil-soluble nanoparticles prepared by the TOP / TOPO method into water-soluble nanoparticles, a very complicated process is required
As far as the preparation of microspheres is concerned, although the swelling method of polystyrene microspheres invented by Nie is simple and feasible, the obtained microspheres are not biocompatible.

Method used

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  • Luminous microsphere and its production method and water dispersion system
  • Luminous microsphere and its production method and water dispersion system
  • Luminous microsphere and its production method and water dispersion system

Examples

Experimental program
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preparation example Construction

[0021] The invention provides a method for preparing fluorescent microspheres, comprising:

[0022] Composite thermosensitive polymer microspheres with water-soluble inorganic fluorescent nanoparticles modified with functional groups on the surface;

[0023] subjecting the resulting complex to an induced phase transition;

[0024] The complexes induced by the phase transition were separated to obtain fluorescent microspheres.

[0025] Heat-sensitive polymers suitable for use in the present invention include, but are not limited to, N-isopropylacrylamide-based polymeric materials; preferably homopolymers of N-isopropylacrylamide and N-isopropylacrylamide and other free Copolymers of free radical monomers, wherein said other free radical monomers include but not limited to acrylamide, acrylic acid, methacrylic acid, N, N'-methylenebisacrylamide or two or more of them mixture.

[0026] The heat-sensitive polymer microspheres suitable for the present invention are preferably hy...

Embodiment

[0050] In the following embodiments of the present invention, three representative fluorescent nanoparticles are used, and the wavelengths of the central positions of the fluorescence emission peaks are 535 nm, 560 nm, and 610 nm (respectively denoted as CdTe535, CdTe560, and CdTe610). The pNIPAM-CdTe composite fluorescent hydrogel microspheres obtained by using CdTe535, CdTe560, and CdTe610 particles are respectively marked as pNIPAM-CdTe535, pNIPAM-CdTe560, and pNIPAM-CdTe610. The composite fluorescent hydrogel microsphere obtained by simultaneously compounding CdTe535 and CdTe610 microparticles into pNIPAM microspheres is expressed as pNIPAM-CdTe535-CdTe610.

[0051] The following examples are used to further illustrate the specific preparation process of fluorescent microspheres, and do not limit the conditions of the preparation process of fluorescent microspheres in the present invention.

[0052] Example of pNIPAM microsphere preparation:

preparation Embodiment 1

[0054] Preparation of N-isopropylacrylamide homopolymer (pNIPAM) microspheres. Weigh 1.53g of N-isopropylacrylamide (NIPAM) monomer and dissolve it in 125ml of secondary deionized water, pass through high-purity nitrogen to remove oxygen for 30 minutes, heat the solution to 70°C to form solution I; weigh 0.076g of persulfuric acid Potassium (KPS) was dissolved in 25ml of secondary deionized water, and high-purity nitrogen was introduced for 30 minutes to form solution II; solution II was added to solution I, and under nitrogen protection, the mixed solution was stirred at 70°C for 4.5 hours to obtain pNIPAM ball.

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Abstract

The invention provides a method for produing fluorescent microsphere, which contains: mixing thermo-sensitive polymer microspheres with water-soluble inorganic fluorescent nanometer particles with function group on the surface; evoking phase change of the composite; separating the phase-changed composite to prepare fluorescent microsphere. And it also provides the fluorescent microsphere and its water disperse system prepared by the method. The invention is of mild reaction condition, simple preparing process and convenient operation, which is suitable for scaled and commercial production, especially for preparing fluorescent marking microsphere for biomedicine check. The prepared fluorescent microsphere is in the nanometer dimensional range; grain size and appearance is homogeneous; luminous intensity is high; luminous purity is nice, dispersion is steady in water and have fine biocompatibility.

Description

Technical field: [0001] The present invention relates to a method for preparing fluorescent microspheres, fluorescent microspheres and their water dispersion system, more specifically, to a polymer / inorganic nanoparticle composite fluorescent compound that can be stably dispersed in water and has biocompatibility. The preparation method of the microsphere, and the fluorescent microsphere prepared by the method and its water dispersion system. Background technique: [0002] Compared with traditional organic dyes, the fluorescent properties of semiconductor nanoparticles have many advantages, such as: wide excitation spectrum, large Stokes shift of fluorescence compared with absorption, symmetrical fluorescence emission characteristics, high fluorescence stability The unique optical properties of fluorescent semiconductor nanoparticles make them have great application prospects in the field of biomedicine as bioluminescent labeling materials, especially in high-throughput biol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/08
Inventor 高明远巩雁军汪大洋
Owner INST OF CHEM CHINESE ACAD OF SCI
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