Genotype detection kit for individual administration and application method thereof

A technology for detecting kits and genotypes, which is applied in the field of genes, can solve the problems of slow detection speed, difficult promotion to clinical application, poor technical stability and poor reliability of results, etc., so as to reduce the number of times, reduce the incidence of adverse drug reactions, alleviate Effects of Pain and Financial Burden

Inactive Publication Date: 2005-11-02
周宏灏 +1
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Problems solved by technology

Most of the genotyping technologies used in laboratories at home and abroad are sequencing, fluorescent PCR, gene chips, etc. These detection methods have disadvantages such as high price, slow detection speed, poor technical stability and result reliability, and complicated operation, so they are not easy to popularize to clinical application

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  • Genotype detection kit for individual administration and application method thereof

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Embodiment Construction

[0013] The present invention will be further described in detail below by taking the genotype detection kit for individualized drug treatment of ulcer disease as an example.

[0014] Peptic ulcer is a common disease, mostly occurs in the stomach or duodenal bulb, collectively referred to as "ulcer disease". Ulcer disease is more common in young adults, or people with a tense pace of life and irregular diet. About 80% of the patients are under the age of 40, and this age is in the energetic stage. Therefore, the prevention and treatment of ulcer disease is very important. People and society are important. According to a large number of statistics, ulcer disease is the most common disease causing upper gastrointestinal bleeding. The prevalence of ulcer disease in the Chinese population is 5-10% (the prevalence ratio of male to female is 3.6:1). The incidence of ulcer disease is on the rise. It is conservatively estimated that the number of patients with ulcer disease in my co...

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Abstract

The present invention belongs to gene technique domain, relates to genotype detection reagent box for individuation using drug and its application method. It is characterized in of the reagent box that comprises: (1) DNA abstract reagent; (2) PCR reagent, wherein, comprises forward primer, reverse primer, both of which can make amplification production to be gene fragment contained drug metabolism and corresponding effect gene mutation site; (3) incision enzyme reagent, comprises inside excision enzyme and incision enzyme buffer liquid; (4) electrophoresis reagent, comprises agarose, bromoethane and buffer liquid with sample; (5) standard substance, comprises wild type plasmid DNA and mutant plasmid DNA. The method of the invention is characterized in that: get peripheral blood of sufferer 1~2ml, abstract DNA by DNA abstract reagent, take PCR amplification by PCR reagent, take limited digestion to inside excision enzyme by incision enzyme reagent, use electrophoresis reagent to prepare agarose gel; take electrophoresis analysis for gel, decide gene type and raise advice for individuation using drug.

Description

technical field [0001] The invention belongs to the field of gene technology, and relates to an individualized drug genotype detection kit and an application method thereof. Background technique [0002] Individual differences in drug efficacy and adverse reactions are common phenomena in the current drug treatment process. Advances in pharmacogenetics and pharmacogenomics have shown that genetic variation in drug-metabolizing enzymes, transporters, and receptors (targets of drug action) is the main cause of individual differences in drug response. If the gene mutation of cytochrome oxidase CYP2D6 occurs, under the same dosage conditions, the blood concentration of the β-receptor blocker mediated by it is 2-3 times higher in the mutant homozygous than in the wild-type homozygous, such as If the dose is not adjusted according to the genotype, severe toxic side effects may occur in patients with homozygous mutants; on the contrary, if the functional mutation of the β receptor...

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Application Information

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IPC IPC(8): G01N27/447
Inventor 周宏灏赵震宇
Owner 周宏灏
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