Genotype detection kit for individual administration and application method thereof

Abstract

The present invention belongs to gene technique domain, relates to genotype detection reagent box for individuation using drug and its application method. It is characterized in of the reagent box that comprises: (1) DNA abstract reagent; (2) PCR reagent, wherein, comprises forward primer, reverse primer, both of which can make amplification production to be gene fragment contained drug metabolism and corresponding effect gene mutation site; (3) incision enzyme reagent, comprises inside excision enzyme and incision enzyme buffer liquid; (4) electrophoresis reagent, comprises agarose, bromoethane and buffer liquid with sample; (5) standard substance, comprises wild type plasmid DNA and mutant plasmid DNA. The method of the invention is characterized in that: get peripheral blood of sufferer 1~2ml, abstract DNA by DNA abstract reagent, take PCR amplification by PCR reagent, take limited digestion to inside excision enzyme by incision enzyme reagent, use electrophoresis reagent to prepare agarose gel; take electrophoresis analysis for gel, decide gene type and raise advice for individuation using drug.

Description

technical field [0001] The invention belongs to the field of gene technology, and relates to an individualized drug genotype detection kit and an application method thereof. Background technique [0002] Individual differences in drug efficacy and adverse reactions are common phenomena in the current drug treatment process. Advances in pharmacogenetics and pharmacogenomics have shown that genetic variation in drug-metabolizing enzymes, transporters, and receptors (targets of drug action) is the main cause of individual differences in drug response. If the gene mutation of cytochrome oxidase CYP2D6 occurs, under the same dosage conditions, the blood concentration of the β-receptor blocker mediated by it is 2-3 times higher in the mutant homozygous than in the wild-type homozygous, such as If the dose is not adjusted according to the genotype, severe toxic side effects may occur in patients with homozygous mutants; on the contrary, if the functional mutation of the β receptor...

AI Technical Summary

Problems solved by technology

Most of the genotyping technologies used in laboratories at home and abroad are sequencing, fluorescent PCR, gene chips, etc. These detection methods have disadvantages such as high price, slow detection speed, poor technical stability and result reliability, and complicated operation, so they are not easy to popularize to clinical application

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