Method for extracting trypsase in intestinal alimentary canal of chicken

The technology of trypsin and extraction method is applied in the field of extraction of trypsin from the digestive tract of chicken intestine, which can solve the problems of long production time, single raw material and high manufacturing cost, and achieve the effects of improving activity and high enzyme yield.

Inactive Publication Date: 2005-11-16
福建圣农控股集团有限公司 +1
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  • Description
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AI Technical Summary

Problems solved by technology

In the past, trypsin and chymotrypsin (chymotrypsin, chymotrypsin) were extracted from the pancreas of mammals, such as pigs and cattle. Now the production of trypsin is mainly based on pig pancreas, and the main process step is acid solution extraction. , graded salting separation, dialysis chromatography purification, the main problems are single raw material, long production time and high manufacturing cost

Method used

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Embodiment Construction

[0006] Below in conjunction with embodiment its processing step is specifically described:

[0007] (1) Wash and crush the chicken intestines to make a slurry. During this process, the temperature should be controlled at 10-15°C;

[0008] (2) Acid solution extraction: select an acid extraction solution with a pH value of 2.5-6.0 away from the PI value of trypsin, and the acid extraction agent can be Na 2 HPO 4 -NaH 2 PO 4 buffer, citric acid-sodium citrate buffer, citric acid-sodium hydroxide-hydrochloric acid buffer or acetic acid acidified solution, the extraction time is 4-6 hours, and the extraction temperature is 10-15°C.

[0009] (3) Salt solution: add solid (NH 4 ) 2 SO 4 , when the saturation is 0.3-0.5sat, the protease can be dissolved in the normal salt solution to the maximum extent, and the impurity protein is discarded at the same time;

[0010] (4) Isoelectric point salting out: use 5mol / L NaOH solution to adjust the pH value, close to the PI value of try...

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PUM

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Abstract

A process for extracting trypsin from the chicken intestine includes cleaning chicken intestine, pulveizing, extracting in acid solution, salting in, isoelectric salting out, activating zymogen, back extracting, desalting for purifying, crystallizing and drying.

Description

technical field [0001] The invention relates to a method for extracting chicken intestinal digestive tract trypsin. Background technique [0002] Trypsin is a proteolytic enzyme and endopeptidase present in the pancreas of animals. In the past, trypsin and chymotrypsin (chymotrypsin, chymotrypsin) were extracted from the pancreas of mammals, such as pigs and cattle. Now the production of trypsin is mainly based on pig pancreas, and the main process step is acid solution extraction. , grading, salting-out separation, and dialysis-chromatography purification, the existing problems are mainly single raw material, long production time and high manufacturing cost. Contents of the invention [0003] The purpose of the present invention is to provide a method for extracting trypsin from the intestinal alimentary canal of chickens, and promote the development of trypsin extraction from mammals and fish to birds. The invention provides a new resource for obtaining trypsin by taki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/64
Inventor 叶永在
Owner 福建圣农控股集团有限公司
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