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Preparation method of high activity complex enzyme preparation used for caragana microphylla degradation

A compound enzyme preparation and high-activity technology, applied in biochemical equipment and methods, enzymes, applications, etc., can solve the problems of low enzyme activity, weak strength, and low utilization rate, and achieve the benefits of enzyme production, easy to master, and low cost. low effect

Inactive Publication Date: 2006-01-25
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The industrial production of compound enzyme preparations in my country is still immature, and the enzyme preparation industry needs to be further developed. Enzyme preparation products still have low enzyme activity, high cost, and low utilization rate, especially in terms of development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Incline cultivation:

[0023] Insert Coriolus versicolor into a test tube of sucrose potato solid medium (potato 20%, sucrose 2%, agar 2%, and the rest is water), put in a 28°C incubator, and cultivate for 10 days;

[0024] (2) Preparation of spore suspension

[0025] Washing the spores of Coriolus versicolor on the slant medium with sterile water to obtain a spore suspension;

[0026] (3) Shake flask expansion culture

[0027] Insert the spore suspension of an inclined test tube in 500 milliliters of triangular flasks with 100 milliliters of sucrose potato nutrient solution (20% of potatoes, 2% of sucrose, 1% of yeast extract, and the rest is water), carry out expanded cultivation, put at 28°C, Rotating speed is 130r / min shaker, culture 8 days, obtain the shake flask spore suspension;

[0028] (4) Enzyme-producing solid fermentation

[0029] Take 100g of the above-mentioned solid medium, after autoclaving, inoculate 4ml of shake flask spore suspension, and then...

Embodiment 2

[0032] The difference from Example 1 is:

[0033] Insert Coriolus versicolor into a test tube of sucrose potato solid medium, place it in a 30°C incubator, and cultivate it for 12 days; add 100 ml of sucrose potato culture medium into the spore suspension, expand the culture, place it at 32°C, Rotating speed is 110r / min shaker, culture 10 days, get shake flask spore suspension; When producing enzyme solid fermentation, take solid culture medium 50g, inoculate 2ml shake flask spore suspension, then adopt dual-temperature cultivation method, ferment for 470 hours, The first 70 hours of constant temperature at 37 ° C, the next 400 hours of constant temperature at 28 ° C, and the ambient humidity is controlled at 50%, so that a high-activity compound enzyme preparation can be prepared. Wherein: the formula of solid medium is: corn steep liquor 15.0%: wheat bran: 23.5%, (NH 4 ) 2 SO 4 : 12.0%, moisture: 49.5%, pH: natural, about 6.4.

[0034] The composite solid enzyme preparat...

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PUM

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Abstract

The invention relates to wood feedstuff, especially production feed that contains lignin and high assay raw fiber. It inoculates the fungus liquid of whiterot fungus to fixed culture medium and takes double temperature cultivating method. Under the ambient humidity 20-50%, natural aeration filling oxygen, compounding enzyme agent would be made. The fixed culture medium is 8-15% corn steep liquor, 20-35% wheat bran, 5-15% (NH4)2SO4, and the other is water. Double temperature cultivating method has two stages: first, 50-80 hours and 32-38 degree centigrade; second, 350-420 hours and 28-32 degree centigrade, totally 400-500 hours. The advantage of the invention is high activity enzyme production, low cost and good effect for feeding livestock.

Description

technical field [0001] The invention relates to a woody feed, especially a production feed containing lignin and high-content crude fiber, in particular to a preparation method of a high-activity compound enzyme preparation for degrading woody feed caragana. Background technique [0002] One of the main limiting factors for the development of animal husbandry in my country is the lack of feed, especially in the arid and semi-arid pastoral areas in western my country. Woody feed is a kind of feed resource, which occupies an important position in animal husbandry feed. Compared with herbal feed, woody feed contains higher nutrients, especially high protein nutrition and calcium content, so it can be used in combination with herbal feed to make up for the lack of protein. In addition, woody feed plays a more obvious role in winter and early spring grass shortage season as livestock feed, which can well alleviate the contradiction of lack of feed during this period. [0003] C...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N1/20A23K1/00A23K10/12
Inventor 李培军许华夏张海荣靖德兵张春桂寇振武丁建国李颖梅
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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