Mononal antibody against avermectin, hybridoma cell line secreting the same and its preparation method

A hybridoma cell line, anti-avermectin single technology, applied in the field of immunology, can solve the application prospect discount of immunodetection method, polyclonal antibody does not have unlimited supply, polyclonal antibody homogeneity, specificity is inferior to monoclonal antibody. Cloning antibodies, etc.
CN1733910AInactive Publication Date: 2006-02-15NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2006-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a novel Avermectin resistant monoclonal antibody, hybridomas cell line excreting it and its preparing process, which comprises synthesizing artificial antigen AVM-BSA, immuning BALB / c mouse for five times, carrying out cell fusion to mouse spleen cells and SP2 / O marrow tumor cells, using PEG1450 as fusion agent to screen positive fused cells, subcloning five times with limited dilution method, obtaining hybridomas cell lines 2F2 specifically secreting AVM antibody, whose micro-organism Deposit number is CGMCC NO.1413, injecting BALB / c mouse's abdominal cavity with the hybridomas cell, gathering ascitic fluid, centrifuging, gathering supernatant fluid, purifying and identifying to obtain the Avermectin resistant monoclonal antibody.
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Description

technical field

[0001] The invention relates to a monoclonal antibody, in particular to an anti-abamectin monoclonal antibody, a hybridoma cell line secreting it and a preparation method, belonging to the field of immunology. Background technique

[0002] Avermectin (AVM) is currently the most widely used anti-endoparasite drug in veterinary clinics, and it is also an agricultural insecticide widely used in agricultural production. Since the first discovery in 1970 by scientists of Merk Sharp and research laboratory in the United States in the screening of soil actinomycete samples from Kitasato Research Institute in Japan, AVMs have been widely used worldwide (Egerton J.E, et al, 1979). As an antiparasitic drug, AVMs has a novel chemical structure and a unique mechanism of action. Due to its specific anthelmintic effect and high safety, it is regarded as the most excellent and most widely used antiparasitic drug for veterinary use with high efficiency and low toxicity. Pa...

Claims

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