Saccharomyce engineering strain for expressing cbh1 gene and its construction method

A technology of genetically engineered strains and yeasts, applied in the field of bioengineering, can solve problems such as increased product cost, poor thermal stability, and short shelf life
CN1757710AInactive Publication Date: 2006-04-12SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2006-04-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

A genetically engineered yeast Pichia pastoris GS-H is prepared through screening the thermophilic Chaetomium CT2 by PCR method to obtain cellobiohydrolase II gene, cloning it, inserting it in the expression carrier of Pichia yeast, introducing it to Pichia yeast, and screening said genetically engineered yeast. It can generate a great deal of active cbhl protein to convert the rejected fiber material.
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Description

(1) Technical field:

[0001] The invention relates to bioengineering, in particular to a Pichia pastoris engineering strain (Pichia pastoris GS-D) expressing cbh1 gene and its construction method. (2) Technical background:

[0002] Cellulose is a cheap renewable resource and the main component of the cell wall of higher plants. Its content reaches 35%-55% of the dry weight of the plant, and it exists widely in nature. Cellulose is hydrolyzed into glucose, and then fermented to produce organic chemical raw materials and fuels such as ethanol, acetone, butanol, as well as feed, food, and medicine. Cellulosic materials are the most promising resources to solve food problems, energy problems and environmental problems faced by human beings. Research and development of cellulose resources has far-reaching significance. Cellulase can cut the molecular chain of cellulose and convert it into glucose. In addition to being fermented and further converted into protein and fat for peop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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