Method for embedding, dehydrating and storing kelp filament

Abstract

The invention discloses a preservation method for sea-tangle filament by embedding and dehydrating, which in detail is an ultra-low temperature storage method applying the sea-tangle filament as freezing material, comprising the following steps: embedding the material, pre-culturing in the sucrose solution, drying and dewatering, pre-freezing, storing in the liquid nitrogen, resuscitating, fixing; the invention overcomes the shortages in the existing filament subculture and storing method and in the two-step ultra-low temperature storing technique, providing a reliable, simple and effective seed storing method used for the sea tangle(L.japonica) with the greatest economic value, which solves the problem of pollution, confounding, and variation in the sea tangle seed storing, the nonuse of deicing fluid avoiding the toxic influence to the seed material and simplifying the step of removing the deicing fluid after the resuscitating; the simple equipment is favorable for the popularity and application of the technique and the survival rate is increased.

Description

1. Technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a cryopreservation technology of macroeconomic algae-kelp germplasm. 2. Background technology [0002] Kelp (Laminaria japonica Aresch) is an important economic algae in China. The life history of kelp is divided into two distinct generational stages: the sporophyte generation and the gametophyte generation. Separation culture can form asexual reproduction lines. The traditional germplasm preservation technology is to subculture and preserve the clones. The traditional preservation technology has obvious deficiencies: (1) liquid culture is the only preservation method, and the method is single, which limits the scale and efficiency of preservation; (2) liquid culture Preservation generally needs to update the culture medium within half a month, the workload is heavy, the operation is cumbersome, and frequent operations also increase the chance of germplasm ...

AI Technical Summary

Problems solved by technology

The traditional germplasm preservation technology is to subculture and preserve the clones. The traditional preservation technology has obvious deficiencies: (1) liquid culture is the only preservation method, and the method is single, which limits the scale and efficiency of preservation; (2) liquid culture Preservation generally needs to update the culture medium within half a month, the workload is heavy, the operation is cumbersome, and frequent operations also increase the chance of germplasm contamination and mixing; (3) the kelp species cannot be aseptically treated before the spores are collected, and the entire preservation process is difficult. Under the condition of bacteria, the pollution of microorganisms, especially algae, cannot be completely eliminated; (4) Abnormal cell morphology and parthenogenesis occur from time to time during culture, and the culture factors need to be further optimized

In ultra-low temperature cryopreservation, effective cryopreservation procedures vary greatly depending on the species. The establishment of the cryopreservation program mainly depends on empirical experimental results. No program is suitable for other species or most species. One of the main difficulties of technology