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Protein and sugar binding protein with anti-HIV activity

A protein-binding and active technology, applied in the direction of antiviral agents, the use of vectors to introduce foreign genetic material, peptide sources, etc., can solve the problems of weak physical defense ability and poor motility, and achieve low cytotoxicity and good resistance to human acquired immunity Effect of systemic syndrome virus activity

Inactive Publication Date: 2006-05-31
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Marine invertebrates have weaker physical defenses (e.g. moving, etc.) as most of them are less mobile

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] At 3-5°C, extract marine invertebrate worms (Serpula lacrymans, Serpulavermicularis or Serpula rhizomorphs) with 0.01-0.1M phosphate buffer (pH 6-9) containing 0.9% NaCl for 12 hours; salt extraction The solution was added to a cross-linked ovomucin-mucin (protein mass ratio 3:1) affinity chromatography column, and the loading buffer was 0.01-0.1M phosphate buffer (pH6-9). Then eluted with water to obtain the protein elution peak; the protein elution peak was concentrated and lyophilized; the lyophilized powder was dissolved in 0.01-0.1M phosphate buffer (pH 6-9), containing 0.9% NaCl, and the solution was added to Go to the molecular sieve chromatography Sephadex G-200 (1.8×98cm); detect the protein elution peak, the first protein elution peak has blood coagulation activity, freeze-dry the elution component with blood coagulation activity, and the freeze-dried powder is sugar binding protein.

[0035] The N-terminus of the sugar-binding protein by conventional amino a...

Embodiment 2

[0040] According to the primers designed according to the amino acid sequence of the N-terminal sugar-binding protein, and the gene sequence transcribed according to the designed primers, the crude product of the sugar-binding protein obtained through genetically engineered bacteria; then cross-linked ovomucin affinity chromatography , ion exchange chromatography and molecular sieve chromatography, obtain pure product; Concrete process is as follows:

[0041] According to the N-terminal amino acid sequence: alanine-aspartic acid-threonine-phenylalanine-cysteine-glutamine-methionine-leucine-glycine-serine-arginine- Threonine-Glycine-Tyrosine-Arginine, use 3′RACE method to amplify full-length cDNA:

[0042] 1) According to the N-terminal amino acid sequence, the 5′-terminal nucleotide sequence was deduced according to standard translation guidelines: 5′GCNGAYACNT TYTGYCARAT GYTNGGNWSN MGNACNGGNT AYMGN 3′;

[0043] 2) Use Primer premier 5 to design a sense gene-specific primer (...

Embodiment 3

[0052] At 4°C, extract 150 mg of marine invertebrates (Serpula lacrymans, Serpulavermicularis or Serpula rhizomorphs) with 50 ml of 0.01-0.1M phosphate buffer (pH6-9), containing 0.9% NaCl, for 12 hours; The extract is added to a cross-linked ovomucin-mucin (protein mass ratio 3:1) affinity chromatography column, and the loading buffer is 0.01-0.1M phosphate buffer (pH 6-9). Then eluted with water to obtain the protein elution peak; the protein elution peak was concentrated and freeze-dried; the freeze-dried powder was dissolved in 0.01-0.1M phosphate buffer (pH 6-9), containing 0.9% NaCl, and 1.5 ml of the Add the solution to molecular sieve chromatography Sephadex G-200 (1.8×98cm); detect the protein elution peak, the first protein elution peak has coagulation activity, and the elution component with coagulation activity is lyophilized, and the lyophilized powder is sugar-binding protein. 4 mg of the sugar-binding protein was produced. As detected by SDS polyacrylamide gel...

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Abstract

A protein and sugar combined protein with anti-HIV activity is from Serpulalacrymans, Serpulavermicularis or Serpula rhizomorphs. It has anti-virus of human acquired immune system syndrome activity and amino acid sequence of SEQ ID No.1. It has no-toxic for human T lymphocytes system C8166 cell. It can express the sugar protein in yeast by 15 amino acid sequences at N end. Its structural analog is an anti-AIDS medicinal guiding compound.

Description

technical field [0001] The present invention is a kind of sugar-binding protein from marine invertebrates (Serpula lacrymans, Serpula vermicularis or Serpula rhizomorphs) anti-human acquired immune system syndrome virus activity and preparation thereof, specifically a kind of glycoprotein in human acquired immune system (Lectin) sugar-binding protein with anti-human acquired immune system syndrome virus activity and preparation thereof during acute infection of syndrome virus and during cell fusion during chronic infection. Background technique [0002] In recent years, due to the emergence of many new viruses that seriously threaten people's health, such as SARS coronavirus, human acquired immune syndrome virus (HIV), avian influenza virus (H5N1) and Ebola virus (Ebola virus) and so on. This forces people to search for new compounds that can be used as drugs. Marine organisms, especially marine invertebrates, are gradually becoming the source of new pharmaceutical compound...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61P31/18C12N15/81C12P19/34
Inventor 李伟郑永唐王建华赵小明张付云李曙光白雪芳杜昱光
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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