2 results about "Sugar binding" patented technology

This invention discloses a highly specific and sensitive immunochromatographic test strip, comprising a first conjugation pad, a second conjugation pad, a nitrocellulose membrane coated with C and T lines, an absorption pad, and a base plate. The first conjugation pad is immobilized with antibody A conjugated to oxidized dextran, representing the antigen to be detected. The second conjugation pad is immobilized with ConA-conjugated colloidal gold and anti-DNP antibody conjugated to colloidal gold. The T line is coated with antibody B, representing the antigen to be detected. The C line is coated with DNP. The invention also discloses the preparation method of the test strip, the detection kit, and the detection method. This invention introduces the dextran and ConA system. ConA is a tetrameric globulin, with each subunit containing one sugar-binding site, and each ConA having four sugar-binding sites, thereby enhancing the detection signal, amplifying the signal, and improving detection sensitivity. It is suitable for the detection of various product types and has broad application prospects.

The application belongs to the technical field of nanometer hole single molecule detection and biochemistry, and provides a nanometer hole based on zebra fish protein and application thereof in single molecule detection. The zebra fish protein is wild type JAC5 protein, and the nanometer hole based on the wild type JAC5 protein is applied to single molecule detection. Compared with other nanometer hole proteins, the wild type JAC5 protein has significant advantages in cloning expression and purification process, and is suitable for detecting nucleic acid, protein and pathogen, and has wide application scenarios. Compared with the wild type JAC5 protein, the mutant zebra fish protein after amino acid mutation of the wild type JAC5 protein can normally express, enhance structural stability and improve detection performance by eliminating the steric hindrance of the sugar binding region to enhance the affinity, or by introducing positive charges to enhance the electrostatic interaction between the transmembrane region and the phospholipid bilayer membrane.