24 results about "Sugar binding" patented technology

The invention relates to the technical field of biosensors, in particular to a noninvasive saliva glucose sensor based on an Au-CeO2 / CNT ternary interface compound and a preparation method of the noninvasive saliva glucose sensor. An Au-CeO2 / CNT ternary interface compound is loaded on the surface of the working electrode; the ternary interface compound comprises oxidized multi-walled carbon nanotubes, cerium oxide nanoparticles loaded on the surfaces of the oxidized multi-walled carbon nanotubes, and gold nanoparticles deposited on the interfaces of the oxidized multi-walled carbon nanotubes and the cerium oxide nanoparticles. A ternary interface compound is loaded on the surface of a working electrode, precious metal gold on the compound serves as a catalytic active site to adsorb glucose, active hydroxyl is generated in combination with rich oxygen vacancies of CeO2, H atoms on glucose-CHO are removed, the catalytic oxidation reaction rate of glucose is increased, and the catalytic oxidation efficiency of glucose is improved. The electron transfer efficiency of the compound is improved by compounding and oxidizing the multi-walled carbon nanotube carrier.

Methods are provided for attaching a mannose-binding C-type lectin receptor targeting moiety to a polymeric carbohydrate scaffold using an amide bond, which can be found between a leash, such as an amine-terminated leash, and the mannose-binding C-type lectin receptor targeting moiety. The disclosed compounds and compositions utilizing this amide bond provide highly stable compounds that significantly reduce loss of the mannose-binding C-type lectin receptor targeting moiety from the polymeric carbohydrate scaffold.

To observe binding with a psyllium seed coat and sugar.SOLUTION: The method for measurement includes: a first selection step of selecting an absorption band that allows a psyllium seed coat to be distinguished from other raw materials; a second selection step of selecting an absorption band that allows sugar to be distinguished from other raw materials; a first measurement step of measuring an absorption spectrum of the composition of the psyllium seed coat in a wider range of the wavenumber, the spectrum including the absorption bands selected in the first and second selection steps; a first data processing step of extracting the absorbance in the absorption bands selected in the first selection step and in the second selection step on the basis of the absorption spectrum obtained in the first measurement step; a second measurement step of determining the detection lower limit of psyllium by using the absorption band selected in the second selection step; a third measurement step of determining the detection lower limit of sugar by using the absorption band selected in the first selection step; a second data processing step of digitizing the distribution of the psyllium seed coat and the sugar by using the detection lower limits determined in the second and third measurement steps from data obtained in the first data processing step; and a calculation step of calculating the ratio of binding of the psyllium seed coat and the sugar from the distribution digitization data.SELECTED DRAWING: None

The invention discloses a yeast immunochromatography rapid detection test strip and a preparation method thereof, and relates to the technical field of immunodetection.The test strip comprises a functionalized sample pad, a combination pad, a reaction film and a water absorption pad which are in lap joint in sequence; the sample pad is pretreated by a treating fluid containing beta-1, 3-glucanase, casein and TritonX-100, so that in-situ wall breaking and interference resistance can be realized; the reaction film detection line is coated with mannose binding lectin and a saccharomycetes specific monoclonal antibody, the binding pad fixes different epitope detection antibodies labeled by colloidal gold, the preparation method comprises the steps of functionalizing the sample pad, coating the reaction film, preparing the binding pad and assembling the test strip, and the matched kit comprises the test strip and a phosphate buffer diluent with a specific pH value. The method can realize extraction-free one-step rapid detection of saccharomycetes, has both specificity and stability, and is suitable for multi-scene screening.

Provided are a novel fluorescent protein, a composition for identifying polarization of macrophages, and a method for identifying polarization of macrophages.　A fluorescent protein is chemically modified with a sugar that binds to a C-type lectin receptor, the fluorescent protein being characterized in that: the sugar that binds to the C-type lectin receptor can bind to C-type lectin receptors expressed in macrophages; and in the fluorescent protein chemically modified with the sugar, the fluorescent protein and the sugar that binds to the C-type lectin receptor are bound directly or via a linker.

Glucose-responsive insulin conjugates that contain one or more linear oligomer sugar cluster are provided. Such insulin conjugates that may display a pharmacokinetic (PK) and / or pharmacodynamic (PD) profile that is responsive to the systemic concentrations of a saccharide such as glucose or alpha-methylmannose, even when administered to a subject in need thereof in the absence of an exogenous multivalent saccharide-binding molecule.

This invention discloses a highly specific and sensitive immunochromatographic test strip, comprising a first conjugation pad, a second conjugation pad, a nitrocellulose membrane coated with C and T lines, an absorption pad, and a base plate. The first conjugation pad is immobilized with antibody A conjugated to oxidized dextran, representing the antigen to be detected. The second conjugation pad is immobilized with ConA-conjugated colloidal gold and anti-DNP antibody conjugated to colloidal gold. The T line is coated with antibody B, representing the antigen to be detected. The C line is coated with DNP. The invention also discloses the preparation method of the test strip, the detection kit, and the detection method. This invention introduces the dextran and ConA system. ConA is a tetrameric globulin, with each subunit containing one sugar-binding site, and each ConA having four sugar-binding sites, thereby enhancing the detection signal, amplifying the signal, and improving detection sensitivity. It is suitable for the detection of various product types and has broad application prospects.

The application discloses a Helicobacter pylori lipopolysaccharide related oligosaccharide antigen and a preparation method and application thereof, and relates to the technical field of organic synthesis.The application discloses a structural formula of the Helicobacter pylori lipopolysaccharide related oligosaccharide antigen, and also discloses a preparation method and application of the oligosaccharide antigen.The oligosaccharide antigen has a clear structure, high purity and uniform components, has no endotoxin toxicity of natural lipopolysaccharide, and is suitable for immunological research and development of a sugar binding vaccine.

A method for separating extracellular vesicles comprises: bringing a sample containing extracellular vesicles into contact with a carrier comprising a ligand for capturing extracellular vesicles; and bringing the carrier after being brought into contact with the sample into contact with a buffer containing a saccharide, wherein the saccharide is at least one saccharide selected from the group consisting of glucose, glucosamine, N-acetylglucosamine, and oligosaccharides and polysaccharides derived therefrom, and the ligand is capable of binding to the saccharide.

The invention belongs to the technical field of cosmetics, and particularly relates to a polygonatum odoratum sugar-binding protein and a preparation method and application thereof. The invention provides the preparation method of the polygonatum odoratum sugar-binding protein, the polygonatum odoratum sugar-binding protein with a specific structure can be obtained by utilizing the preparation method, and the obtained polygonatum odoratum sugar-binding protein has the activity of agglutinating sheep red blood cells, can inhibit the activity of hyaluronidase and elastase, can control inflammatory reaction and anaphylactic reaction, and can be used for preparing the polygonatum odoratum sugar-binding protein. The skin barrier repair is promoted. Results of the embodiment show that the skin cream prepared from the polygonatum odoratum sugar binding protein has a protective effect on cells damaged by ultraviolet rays, and the polygonatum odoratum sugar binding protein can protect skin cells, improve the tolerance of skin to ultraviolet rays and relieve skin aging caused by light damage, so that the anti-aging effect is achieved.

The invention belongs to the technical field of nanopore single molecule detection and biochemistry crossing, and provides a nanopore based on zebra fish protein and application of the nanopore in single molecule detection. The zebra fish protein is wild type JAC5 protein, the nanopore based on the wild type JAC5 protein is applied to single molecule detection, compared with other nanopore protein and wild type JAC5 protein cloning expression and purification processes, the nanopore based on the wild type JAC5 protein has remarkable advantages, the applicable detection range covers nucleic acid, protein, pathogen and the like, and the application scene is wide. Compared with a wild type JAC5 protein and a mutant type zebrafish protein after amino acid mutation of the wild type JAC5 protein, the mutant type zebrafish protein can be normally expressed and enhance structural stability by eliminating steric hindrance of a sugar binding region to enhance affinity or introducing positive charges to enhance electrostatic interaction between a transmembrane region and a phospholipid bilayer membrane; and the detection performance is improved.

The invention provides a novel streptococcus group A oligosaccharide antigen and glycobinding protein vaccine, which is characterized in that oligosaccharide antigen containing 30-70% of N-acetyl glucamine multi-rhamnose core skeleton structure is accurately prepared through chemical synthesis, and is efficiently coupled with KLH (the loading capacity is about 215 oligosaccharide / KLH molecules); safe, efficient and broad-spectrum immune protection is realized; safety: the GlcNAc side chain modification design avoids autoimmune risks (such as acute rheumatic fever heat); high efficiency: the animal experiment antibody titer reaches 1: 12, 800; the broad spectrum is as follows: the highly conservative universal GAS oligosaccharide antigen can universally cover various serotypes, and the key synthetic route (Scheme 2, 3, 16 and 20, the yield is 22%-96%) and the loading capacity verification (HPAEC) support the technical feasibility.

The present invention first discloses a method for preparing a lectin-modified nano-selenium composite material, including the steps of mixing AANL with sodium selenite for the first incubation, mixing with ascorbic acid for the second incubation, coating, centrifuging and discarding the supernatant, resuspending, washing, etc. The present invention also discloses the application of the composite material in sugar binding, especially cell agglutination and / or in anti-oxidation and / or in the preparation of drugs for inhibiting lung cancer cell proliferation. The method of the present invention has low cost, environmental protection, high efficiency, simple preparation method, simple required equipment, and is easy to promote. The composite material provided has good dispersibility, is more stable, has good biocompatibility and solubility, and has significant application effect. It can be better used in the field of glycobiology as a sugar tool; it has better biological activity, strong antioxidant activity and ability to inhibit lung cancer cell proliferation, and has good application prospects in the field of biomedicine.

This disclosure provides a composition containing a conjugate with a modified insulin molecule. The conjugate has an insulin molecule, which can be insulin or an insulin analog, glucagon, GLP-1, GLP-2 or a GLP-1 agonist. The conjugate also contains one or more polymers. Each of the one or more polymers is covalently linked to the insulin molecule. Additionally, each of the one or more polymers is covalently linked to between 0 to 50 copies of a decoy ligand, and to between 0 to 50 copies of a glucose-binding agent, such that the combined total number of glucose-binding agents and decoy ligands covalently linked to each of the one or more polymers is at least 1. The conjugate can reversibly bind to soluble glucose and in which the extent of its glucose-binding controls the extent to which the modified insulin is able to bind to and activate the insulin receptor. Methods of making the conjugate, as well as use of the conjugate in treatment, are also provided.

A tyrosine residue modification method has been developed, which includes: mixing a protein with a glycosylation agent having a sugar conjugated to 1-methyl-4-aryl-urazole (MAUra); and adding laccase to the mixture to modify a tyrosine residue with the MAUra compound. This method prevents protein aggregation and can provide stable protein preparations. The tyrosine modification method using laccase and MAUra is a mild method and enables easy glycosylation without any alteration of an amino acid sequence for sugar conjugation.

The present invention relates to a novel immunogenic composition containing a conjugated Streptococcus pneumoniae capsular sugar antigen (sugar conjugate), a kit containing the immunogenic composition, and the use of the same. The immunogenic composition of the present invention typically contains at least one sugar conjugate derived from a Streptococcus pneumoniae serotype not found in PREVNAR®, SYNFLORIX®, and / or PREVNAR13®. The present invention also relates to the vaccination of human subjects, particularly infants and the elderly, against pneumococcal infection using the novel immunogenic composition.

The invention discloses a method for identifying glycosyltransferase or glycosidase specific enzyme participating in DNA / RNA glycosylation, which is characterized in that oxidation labeling is carried out on glycosylation modification of DNA / RNA molecules, and solid phase coupling and enzyme digestion strategies are combined, so that enzyme or sugar binding protein specifically bound with glycosylation DNA / RNA can be efficiently and accurately captured, and the specific enzyme of glycosyltransferase or glycosidase can be identified. The interference caused by non-specific binding is obviously reduced. The method is not only suitable for various biological samples including tissue slices, blood and cell samples, but also compatible with subsequent mass spectrometric analysis, and high-throughput identification and function annotation of targeted proteins are realized. The technology provides a brand new tool for analyzing the action mechanism of DNA / RNA sugar modification in epigenetic regulation and control, and has important value for promoting the research of related disease mechanisms and the development of targeted therapy strategies.

Provided are compositions that include compositions of galactosyl-conjugated lipid, nanoparticles (LNPs) encapsulating one or more active agents. In some embodiments, the galactosyl-conjugated LNPs have a lipid component having D-Lin-MC3-DMA, ALC-0315 and SM-102, cholesterol, DSPC and DOPE, and DMG-2000-PEG. In some embodiments, the GaIN Ac-conjugated LNP has one or more galactosyl moieties bioconjugated to cholesterol present with a lipid component of the GaINAc-conjugated LNP. Also provided are methods for treating and / or preventing diseases, disorders, and / or conditions associated with undesirable gene expression and methods for targeting active agents to hepatocytes using the presently disclosed GaIN Ac-conjugated LNPs.

The application belongs to the technical field of nanometer hole single molecule detection and biochemistry, and provides a nanometer hole based on zebra fish protein and application thereof in single molecule detection. The zebra fish protein is wild type JAC5 protein, and the nanometer hole based on the wild type JAC5 protein is applied to single molecule detection. Compared with other nanometer hole proteins, the wild type JAC5 protein has significant advantages in cloning expression and purification process, and is suitable for detecting nucleic acid, protein and pathogen, and has wide application scenarios. Compared with the wild type JAC5 protein, the mutant zebra fish protein after amino acid mutation of the wild type JAC5 protein can normally express, enhance structural stability and improve detection performance by eliminating the steric hindrance of the sugar binding region to enhance the affinity, or by introducing positive charges to enhance the electrostatic interaction between the transmembrane region and the phospholipid bilayer membrane.

The invention is suitable for the technical field of food processing, and provides a low-GI meal replacement bar and a preparation method thereof. The low-GI meal replacement bar is prepared from the following raw materials: oat flour, resistant starch, resistant dextrin, psyllium husk powder, low-methoxyl pectin, calcium hydrogen phosphate, fructose, L-glutamine, a mucus penetrating nano compound, a natto extract-chickpea peptide compound, soybean protein isolate, crushed nuts, sodium bicarbonate, citric acid, water, table salt and sucralose. An intelligent physical barrier is constructed through a pH response dual gel sugar control network, mucus penetrates through the nano-composite to achieve targeted nutrition delivery, instant-long-term dual sugar control is achieved through the natto extract-chickpea peptide composite, a flavor precursor directional release technology is combined, and the taste of the natto extract-chickpea peptide composite is improved. The effects that the glycemic index is remarkably reduced, the satiety is lasting, the flavor is natural and no bad aftertaste exists are achieved, a low-temperature mixing and directional curing technology is adopted in the preparation method, and the activity of functional components is effectively protected.

Disclosed is a) a conjugated peptide being conjugated with at least one reducing sugar and a degree of conjugation of at least 10 percent, preferably being in solid form such as powders; b) a method for preparing non-bitter conjugated protein hydrolysates comprising hydrolyzing a protein by at least one enzyme, mixing the resulting protein hydrolysate and at least one reducing sugar, heating the mixture to perform a Maillard reaction and drying the composition; c) a method for masking the bitter taste of a peptide comprising mixing a peptide and a reducing sugar, heating the mixture to perform a Maillard reaction and drying to obtain a powder; and d) the use of a conjugated peptide for food products, dietary supplements, pharmaceutical products and animal feed products.

The present invention provides conjugates comprising a sugar such as mannitol and one or more L-DOPA and / or DOPA decarboxylse inhibitors including, inter alia, L-DOPA, carbidopa, benserazide, or a combination thereof, wherein the sugar is conjugated to the carboxyl group of the L-DOPA and / or DOPA decarboxylse inhibitor / s) via a hydroxyl group of the sugar. The present invention further provides related pharmaceutical compositions and methods of producing the conjugates, as well as methods of use for treating medical disorders responsive to dopamininergic stimulation such as movement disorders including, inter alia, Parkinson's Disease.

The present invention discloses an immunochromatographic test strip for signal amplification, which comprises a first conjugate pad, a second conjugate pad, a nitrocellulose membrane coated with a C line and a T line, a sample absorbing pad and a bottom plate; wherein the first conjugate pad is fixed with antibody A against the antigen to be detected conjugated with oxidized dextran; the second conjugate pad is fixed with ConA-conjugated quantum dot fluorescent microspheres and anti-DNP antibody-conjugated quantum dot fluorescent microspheres; the T line is coated with antibody B against the antigen to be detected; and the C line is coated with DNP. The preparation method of the test strip, a detection kit and a detection method are also disclosed. The present invention introduces a dextran and ConA system. ConA is a tetrameric globulin, and each subunit contains 1 sugar-binding site, and each ConA has 4 sugar-binding sites, thereby enhancing the detection signal, realizing signal amplification and improving the detection sensitivity. It is suitable for the detection of various product types and has broad application prospects.

The invention discloses pacific oyster galactose / rhamnose binding lectin CGL-1 recombinant protein as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. In a diabetic rat model, the CGL-1 recombinant protein can significantly promote the healing of MRSA infectious wounds, and the healing rate reaches 92.44%-94.51%. Histological analysis shows that inflammatory cell infiltration of a wound surface of a CGL-1 treatment group is reduced, collagen deposition is increased, neovascularization is good, and a good healing effect is shown. In addition, the bacterial quantity of a wound surface of a CGL-1 treatment group is obviously lower than that of a model group, and the bacterial quantity is respectively reduced by 25.3%-88.55% and 68.45%-95.83% on the fifth day and the thirteenth day. The results show that the CGL-1 lectin protein has the potential of serving as a novel non-antibiotic therapeutic agent, can be used for treating diabetes infected wounds, and provides a new thought for application of marine biological resources in the field of wound healing.