35 results about "Sugar binding" patented technology

A method for enhancing a binding activity of an antibody composition to Fcγ receptor IIIa, which comprises modifying a complex N-glycoside-linked sugar chain which is bound to the Fc region of an antibody molecule; a method for enhancing an antibody-dependent cell-mediated cytotoxic activity of an antibody composition; a process for producing an antibody composition having an enhanced binding activity to Fcγ receptor IIIa; a method for detecting the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain among total complex N-glycoside-linked sugar chains bound to the Fc region in an antibody composition; an Fc fusion protein composition produced by using a cell resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex N-glycoside-linked sugar chain; and a process for producing the same.

Provided herein are use of polymeric excipients, specifically polyvinyl alcohols, optionally in conjunction with sugars, as cryoprotectants to prevent aggregation of PEG-containing particles. Also provided are PEG-containing particles comprising such polymeric excipients.

The present application belongs to the field of biology, and provides amphioxus C-type lectin BjCTL4 gene and application thereof. The protein encoded by the C-type lectin BjCTL4 gene discovered for the first time in this application, its CTL-like domain has the basic features of traditional CTL, including two or three pairs of conserved disulfide bonds, a motif similar to the characteristic motif WIGL, and with Ca 2+ Similar motifs of related sugar-binding motifs QPD / EPN and WND have been preliminarily verified to be able to extensively bind polysaccharides on the surface of bacteria, and have obvious bacterial binding and bacterial agglutination, which can be developed into natural antibacterial active substances and preparations. Medicines to treat infectious diseases.

The invention belongs to the field of glycochemical biology, and in particular relates to a dithiodimannobiose-gold nanoprobe, a preparation method and its application in the recognition of virus receptor DC-SIGN / R. A dithiobimannobiose-gold nanoprobe is characterized in that: the ligand exchange method is used to modify the dithiobimannobiose ligand on the surface of the gold nanoparticle. Based on click chemistry and ligand exchange, the present invention modifies mannobiose ligands on the surface of gold nanoparticles, constructs a novel dithiodimannobiose-gold nanoprobe, and studies tetrameric lectin DC based on a fluorescence quenching strategy. The sugar binding mechanism of ‑SIGN and DC‑SIGNR lays the foundation for the establishment of a rapid and sensitive method to study the binding affinity and thermodynamics of protein-saccharide multivalent interactions.

A two-chain insulin analogue contains an A chain modified by (i) a monomeric glucose-binding element at or near its N terminus and (ii) a B chain modified by at or near its C terminus by an element that reversibly binds to the monomeric glucose-binding element such that this linkage is displaceable by glucose. The monomeric glucose-binding element may be phenylboronic acid derivative (optionally halogenated). The B chain may be modified by a diol-containing element derived from a monosaccharide, disaccharide or oligosaccharide, a non-saccharide diol-containing moiety or a α-hydroxycarboxylate-containing moiety. The analogue can be manufactured by trypsin-mediated semi-synthesis. Formulations can be at strengths U-10 to U-1000 in soluble solutions at pH 7.0-8.0 with or without zinc ions at a molar ratio of 0.0-3.0 ions per insulin analogue monomer. A patient with diabetes mellitus may be treated with subcutaneous, intraperitoneal, or oral administration of a physiologically effective amount of the insulin analogue.

Sodium cyanoborohydride (NaCNBH 3 ) can reduce the aldehyde group formed by the oxidation of polysaccharide by sodium periodate and the amino group of the protein to form a C=N double bond, which promotes the combination of sugar and protein, thereby being used in the preparation process of polysaccharide-conjugated vaccine; the invention provides a polysaccharide residual NaCNBH in conjugate vaccine 3 Ion Chromatography-Pulsed Amperometric Detection Method, comprising the steps of: using NaCNBH 3 Prepare the reference solution from the standard product; centrifuge the sample to be tested with an ultrafiltration centrifuge tube, collect the filtrate and filter it with a 0.45 μm membrane as the test solution; the test solution and the reference solution NaCNBH 3 Load the sample on the ion chromatographic column respectively, elute after loading the sample, and record the chromatogram; according to the reference substance solution NaCNBH 3 Concentration and its chromatographic peak area, need testing solution NaCNBH 3 Chromatographic peak area, using external standard method to calculate NaCNBH in the sample 3 concentration. The method of the present invention is simple, easy to operate, and high in accuracy, and can accurately and quickly realize the residual NaCNBH in the polysaccharide-conjugated vaccine 3 effective monitoring.

A cancer cell proliferation suppression agent according to the present invention contains, as an active component, an inositol derivative in which inositol is bound to a sugar. In addition, a composition for suppressing proliferation of cancer cells according to the present invention contains the above mentioned cancer cell proliferation suppression agent and a pharmaceutically-acceptable carrier.