High-efficient apple gene conversion

A technology of gene transformation and apples, applied in the field of agricultural biology, can solve the problems of low iron deficiency resistance, low germination rate, and high difficulty, and achieve the effect of solving iron deficiency problems, strong reference, and improving absorption

Inactive Publication Date: 2006-05-31
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, it is not easy to use a gene gun; the use of rootstock is not very ideal, the ability to resist iron deficiency is low, there is a certain repulsion, and it still shows iron deficiency growth
In addition, it is difficult to transfer genes into apple explants, the regeneration of explants is difficult, the germination rate is very low, and the regenerated plants are easily polluted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Embodiments of the present invention are described in further detail below:

[0032] Transformation method of the present invention is made of the following steps:

[0033] 1. Take the shoot tip tissue of apple tree and cultivate it on MS medium

[0034] Get the stem tip tissue of the apple tree, at first adopt the sodium hypochlorite solution of 2.8% chlorine ion concentration to disinfect for 10-15 minutes, then inoculate it on the MS medium for cultivation, and the cultivation time is 1-2 weeks, and the environmental conditions for cultivation are: The temperature is 25-28°C, the light is about 12 hours a day, the light intensity is 2500-5000LUX, and then washed three times with sterile water.

[0035] 2. The cultured shoot tip tissue is re-cultured on the expansion medium

[0036] Inoculate the cultured shoot apex tissue into the expansion medium for 4-6 weeks, and the expansion medium is supplemented with 2-4 mg / L of 6-BA growth hormone and 0.2-0.4 mg / L of MS med...

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PUM

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Abstract

The invention is a high efficiency apple gene transform method and belongs to the field of plant gene engineering. The main technology attribute is to take tissue to put up primary culture on the MS substrate, put up second culture on Multiplication substrate, take agrobacterium with FRO2 gene culture, differentiation culture and relay culture, elongation culture, rooting culture, soil culture. This invention is scientific and rational, its plant has high rebirth and transformation rates; the transgenic apple that grows by this invention has anti-lack iron gene, and can improve the attraction of iron. It solves the lack iron of apple in base, and has referential function in gene transform of other fruit.

Description

[0001] Field [0002] The invention belongs to the field of agricultural biotechnology or the field of plant genetic engineering, in particular to a high-efficiency apple gene transformation method. Background technique [0003] At present, transgenic technology has been widely used in fruit trees. Due to the characteristics of perennial, long childhood, self-incompatibility, high degree of heterozygosity, and multiple asexual reproduction of fruit trees, the use of gene transfer technology to obtain new varieties of transgenic plants has gradually become an important means of fruit tree breeding. The earliest transgenic plants were obtained in 1988 using walnut system as explants and mediated by Agrobacterium. Then researches were carried out on many fruit trees. For example, the ICP gene and CHS gene were successively introduced into the walnut, the homebox gene of rice was transferred into the kiwifruit, and the flowering control gene was transferred into the banana by Sag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H4/00C12N5/10
Inventor 刘艳军杨静慧杨恩芹柳树梧刘玉冬
Owner TIANJIN AGRICULTURE COLLEGE
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