Method for extracting polyhydroxyalkanoates from active sludge
A technology for polyhydroxyalkanoates and activated sludge, which is applied in the field of extracting polyhydroxyalkanoates and can solve problems such as ultrasonic-assisted extraction of PHA
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[0012] Experimental Drugs:
[0013] Activated sludge is taken from Tianjin TEDA Municipal Wastewater Treatment Plant
[0014] Chloroform Analytical Pure Tianjin North Chemical Glass Purchase and Sales Center
[0015] Methanol Analytical Pure Tianjin Fuyu Fine Chemical Co., Ltd.
[0016] Antifomin Analytical Pure Tianjin Chemical Reagent No. 3 Factory
[0017] Glucose, ammonium chloride, potassium dihydrogen phosphate, ZnSO 4 ·7H 2 O, NiCl 2 ·H 2 O, CuSO 4 ·5H 2 O Sudan black, saffron, xylene (all are analytically pure)
[0018] 1. Test steps
[0019] 1. Activated sludge taken from Tianjin Taida Municipal Sewage Treatment Plant, using glucose, ammonia nitrogen and sodium phosphate according to the culture medium prepared under the condition of C: N: P = 100: 1: 1 (weight ratio) to cultivate activated sludge , using aerobic and anoxic alternate domestication sludge, the cycle is 4 hours, the time ratio of aerobic and anoxic state is 1:1.
[0020] 2. Take 3.0L of activ...
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