Method for extracting polyhydroxyalkanoates from active sludge

A technology for polyhydroxyalkanoates and activated sludge, which is applied in the field of extracting polyhydroxyalkanoates and can solve problems such as ultrasonic-assisted extraction of PHA

Inactive Publication Date: 2006-07-19
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the extraction of PHA, there is no report on the use of ultrasonic-assisted extraction of PHA

Method used

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  • Method for extracting polyhydroxyalkanoates from active sludge
  • Method for extracting polyhydroxyalkanoates from active sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Experimental Drugs:

[0013] Activated sludge is taken from Tianjin TEDA Municipal Wastewater Treatment Plant

[0014] Chloroform Analytical Pure Tianjin North Chemical Glass Purchase and Sales Center

[0015] Methanol Analytical Pure Tianjin Fuyu Fine Chemical Co., Ltd.

[0016] Antifomin Analytical Pure Tianjin Chemical Reagent No. 3 Factory

[0017] Glucose, ammonium chloride, potassium dihydrogen phosphate, ZnSO 4 ·7H 2 O, NiCl 2 ·H 2 O, CuSO 4 ·5H 2 O Sudan black, saffron, xylene (all are analytically pure)

[0018] 1. Test steps

[0019] 1. Activated sludge taken from Tianjin Taida Municipal Sewage Treatment Plant, using glucose, ammonia nitrogen and sodium phosphate according to the culture medium prepared under the condition of C: N: P = 100: 1: 1 (weight ratio) to cultivate activated sludge , using aerobic and anoxic alternate domestication sludge, the cycle is 4 hours, the time ratio of aerobic and anoxic state is 1:1.

[0020] 2. Take 3.0L of activ...

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Abstract

The related extraction method for polyhydroxy fatty acid ester from active sludge comprises: centrifuging the sludge to dry the solid and obtain dried active sludge with 5-15% water content; grinding the product into fine grain of 40-10item; adding chloroform by weight ratio with the sludge as 1:25-50; treating with supersonic wave, water-bath oscillating, centrifuging, condensing, and adding methanol of 20-40 times; and centrifuging to separate solid and put into petri dish for drying. This invention benefits to residual sludge treatment and comprehensive utilization, and cuts the extraction time for higher efficiency.

Description

technical field [0001] The invention relates to the field of excess activated sludge treatment and comprehensive utilization, in particular to a method for extracting polyhydroxyalkanoate from activated sludge. Background technique [0002] A large amount of activated sludge is produced in the process of sewage treatment, which has become a huge burden on the current sewage treatment plant. It not only costs a lot of money to process, but also occupies land for the final treatment of sludge, which is easy to cause secondary pollution to the environment. Polyhydroxyalkanoate (PHA) is a substance that stores energy and carbon sources synthesized by bacteria in cells when nutrients are unbalanced. The general formula of polyhydroxyalkanoate is as follows: [0003] [0004] where m=1,2,3. Usually m=1, that is, β-hydroxy fatty acid. R is a side chain, which may be saturated or unsaturated, straight chain or an alkyl group containing side chains and substituents ranging from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/02C02F11/02
Inventor 李克勋
Owner NANKAI UNIV
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