Method for prodcuing D-ribose by inter mass fermentation

A technology of fed-batch fermentation and ribose, which is applied in the field of fed-batch fermentation to produce D-ribose, which can solve the problems of low D-ribose output and excessive residual glucose, and achieve short fermentation and cultivation time, simple ingredients and prolong production time Effect

Inactive Publication Date: 2006-08-09
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low D-ribose yield and excess gluc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ①Slope culture: Put the transketolase-deficient Bacillus subtilis HGD2004 into the slant medium. The formula of the slant medium is: yeast extract, 7.0g / L; peptone, 15.0g / L; sorbitol, 5.0g / L; sodium chloride, 2.0g / L; dipotassium hydrogen phosphate, 2.0 g / L; potassium dihydrogen phosphate, 1.0 g / L; agar, 26.0 g / L. The slope was placed in an incubator for 24 hours, the culture temperature was 36°C, and the initial pH of the slope medium was 7.0.

[0026] ②Seed liquid culture: then transfer the cultured strains to the seed liquid medium, and use the rotary constant temperature and speed-adjustable shake flask cabinet for cultivation; control the speed of 240r / min, the culture temperature of 36℃, the initial pH value of 7.0, and the culture of 12 hour. The formula of the seed liquid culture medium is: yeast extract, 5.0 g / L; dipotassium hydrogen phosphate, 3.0 g / L; potassium dihydrogen phosphate, 1.0 g / L; glucose, 20.0 g / L.

[0027] ③Fermentation culture: Finally, the cultured...

Embodiment 2

[0031] In the step of fermentation and cultivation, the pH value is controlled to 7.0, and the other reaction steps are the same as in Example 1. The fermentation culture time was 72 hours, the D-ribose yield was 63.61g / L, and the remaining glucose concentration was 11.5g / L.

Embodiment 3

[0033] In the step of fermentation and cultivation, the pH value is controlled to 6.5, and the other reaction steps are the same as in Example 1. The fermentation culture time is 64 hours, the D-ribose output is 70.35g / L, and the remaining glucose concentration is 6.5g / L.

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Abstract

This invention relates to a method for producing D-ribose by supplementing substances characterizing in inducing, selecting and cultivating the type of transketolase defective Bacillus subtilis, cultivating them in the slope substrate, the seed liquid substrate and the fermentation substrate, in which, during the fermentation period, a feeding way of intermission degression and a way of quantitative feeding are applied to feed grape sugar supplementary solution and (NH4)2SO4 to get the D-ribose finally.

Description

Technical field [0001] The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for producing D-ribose by fed-batch fermentation. Background technique [0002] D-ribose is a five-carbon sugar with important physiological functions and has a wide range of uses in food and medicine. In the food industry, D-ribose can be used to produce nucleotide flavor enhancers, such as 5 / -inosinic acid, 5 / -guanylic acid, etc.; in medicine, D-ribose has a very wide range of applications. The most widely used is the semi-synthetic method to produce riboflavin. D-ribose itself also has a certain medical effect, such as the treatment of physical weakness caused by cardiac insufficiency and the decline of organ function caused by organ transplantation; due to the special molecular structure of D-ribose, It can be used to synthesize some prodrugs with special spatial structure. [0003] There are currently three production methods for D-ribose: ext...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/125
Inventor 吴兆亮刘虎胡滨郑辉杰
Owner HEBEI UNIV OF TECH
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