Purifying method for soybean oligosaccharide functional factor by biological method
A technology of soybean oligosaccharides and functional factors, applied in the field of food
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Embodiment 1
[0036] (1). Preparation of Arthrobacter fructose transferase:
[0037] (1-1) Incline medium: glucose-0.5%, peptone-0.5%, yeast extract-0.5%, (NH 4 ) 2 HPO 4 -0.1%, agar-1.5g, pH value 6.8. Sterilize at 121°C for 20 minutes, place on an inclined plane to cool, incubate at 28°C for 30 hours after inoculation, and set aside.
[0038] (1-2) Erlenmeyer flask liquid medium and liquid expansion medium:
[0039] Sucrose-20%, peptone-0.5%, yeast extract-0.5%, (NH 4 ) 2 HPO 4 -0.1%, pH 7.0.
[0040] Then sterilize at a high temperature of 121°C, cool to 32°C, inoculate strains, carry out ventilated culture at a temperature of 28°C-32°C for 16 hours, and then transfer to a fermenter for fermentation after maturing.
[0041] (1-3) Arthrobacter fermentation culture:
[0042] Sucrose 20% pH 7.0. Then sterilize at a high temperature of 121°C, cool to 32°C, inoculate strains, and carry out ventilated culture at 32°C for 1 day.
[0043] After the cultivation, centrifuge at 3500-4000 ...
Embodiment 2
[0052] (1). Preparation of Arthrobacter fructose transferase:
[0053] (1-1) Incline medium: glucose-0.5%, peptone-0.5%, yeast extract-0.5%, (NH 4 ) 2 HPO 4 -0.1%, agar-1.5g, pH value 6.8. Sterilize at 121°C for 20 minutes, place on an inclined plane to cool, and incubate at 28°C-32°C for 16-30 hours after inoculation, and set aside.
[0054] (1-2) Erlenmeyer flask liquid medium and liquid expansion medium:
[0055] Sucrose-20%, peptone-0.5%, yeast extract-0.5%, (NH 4 ) 2 HPO 4 -0.1%, pH 7.2.
[0056] Then sterilize at a high temperature of 121°C, cool to 28°C, insert strains, carry out ventilated culture at a temperature of 28°C-32°C for 12-16 hours, and transfer to a fermenter for fermentation after maturing.
[0057] (1-3) Arthrobacter fermentation culture:
[0058] Sucrose 20% pH 7.0. Then sterilize at a high temperature of 121°C, cool to 28°C, inoculate strains, and carry out ventilated culture at 28°C for 4 days.
[0059] After the cultivation, centrifuge at 3500...
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