Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for predicting angiotonin II receptor agonist hypotensor medicine function and its use

A technology for angiotensin and drug action, applied in biochemical equipment and methods, microbiological determination/testing, etc., can solve the problems of unseen bradykinin receptor gene association research, unseen kit products, etc.

Inactive Publication Date: 2006-11-08
ANHUI PROV INST OF BIOLOGICAL MEDICINE +1
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no research on the association between the bradykinin receptor gene and the antihypertensive effect of irbesartan, nor has there been a kit product for irbesartan pharmacogenomics research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for predicting angiotonin II receptor agonist hypotensor medicine function and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Determination of the C-58T polymorphism site of the bradykinin B2 receptor gene and prediction of the antihypertensive effect of angiotensin II receptor 1 antagonist antihypertensive drug irbesartan

[0049] (1) Determination of the C-58T polymorphic site genotype of the bradykinin B2 receptor gene:

[0050] (1) Extract the genomic DNA of the host cell:

[0051] (a) Add 30ml of erythrocyte lysate to the whole blood, shake slowly, and let stand at room temperature for 10 minutes. During this period, shake several times to completely lyse the erythrocytes;

[0052](b) Centrifuge at 4°C at 2000 rpm for 10 minutes, remove the supernatant, break up the precipitated leukocytes on a rotary shaker, add 40ul of protease and 50ul of RNase, shake well, add 15ml of leukocyte lysate, Mix well in a 37°C water bath for 20 minutes, take it out, and put it in cold water;

[0053] (c) Add 4ml of cold protein precipitation solution, mix well and place in -20°C refrigerator fo...

Embodiment 2

[0075] Example 2: Determination of the C-58T polymorphism site of the bradykinin B2 receptor gene to predict the target organ impact of angiotensin II receptor 1 antagonist antihypertensive drug irbesartan

[0076] (1) Measure the C-58T polymorphic site genotype of the bradykinin B2 receptor gene (same as embodiment 1)

[0077] (2) Predict the risk of microalbuminuria:

[0078] For patients with essential hypertension, the plasma triglyceride level of genotype -58C / T heterozygous or -58T / T homozygous mutant individuals was significantly higher than that of genotype -58C / C homozygous wild type individuals.

[0079] The above method was verified by research, and the patients with essential hypertension were divided into groups; the bradykinin B2 receptor genotypes were divided into groups (homozygous wild type group, heterozygous type group + homozygous mutant group), and the occurrence of proteinuria was observed. The result is as follows:

[0080] In patients with essential ...

Embodiment 3

[0082] Embodiment 3: kit composition (Taqman method) and application

[0083] 1. Kit components include:

[0084] Solution A: Taqman 2X Universal PCR Master Mix No AmpErase UNG (composition includes: AmpliTaqGold DNA Polymerase, dNTPs with Dutp, Passive Reference, optimized buffer);

[0085] Solution B: forward primer, reverse primer;

[0086] Liquid C: two allele-specific probes with fluorescent reporter groups,

[0087] Probes carrying the VIC fluorescent reporter group correspond to the "C" allele;

[0088] Probes carrying a FAM fluorescent reporter correspond to the "T" allele.

[0089] 2. The steps of using this kit to amplify functional polymorphic sites and their flanking sequences and detect the genotypes of polymorphic sites are as follows:

[0090] Amplify target gene fragments: In a 5ul PCR reaction system containing 10ng of genomic DNA, 2.5ul of liquid A, 0.72uM of liquid B and 0.16uM of liquid C, carry out the PCR reaction according to the following conditions...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a kind of reagent kit including reagent for detecting the polymorphic site genotype of bradykinin receptor gene of nucleic acid template. By means of PCR-RFLP method or Taqman method, the detection is fast, safe, convenient, sensitive and reliable. The detection can predict the hypotensive effect of angiotonin II receptor 1 agonist hypotensor and predict the blood fat state of the primary hypertension patient, so that the medicine may be selected based on the individual difference to raise clinical treating efficiency and safety and lower the toxic side effect risk and treating cost.

Description

technical field [0001] The invention relates to a method for predicting the action of angiotensin II receptor antagonist antihypertensive drugs and its application. By measuring the genotype of a specific site in a biological sample, the angiotensin II receptor 1 antagonist antihypertensive drugs are predicted drug effect. Background technique [0002] Hypertension is one of the most common chronic diseases and a major risk factor for stroke, coronary heart disease, diabetes, etc. Therefore, effective control of blood pressure has important clinical and public health significance. There are six types of drugs clinically used to treat hypertension, including: angiotensin II receptor (AT1-R) antagonists, β-blockers, angiotensin converting enzyme inhibitors (ACEI), calcium antagonists , α-adrenoceptor blockers and diuretics. But so far the control rate of hypertension is low, the data of our country and the United States show that the control rate is only 8.1% and 34% respect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 张善春王琳琳张岩洪秀梅毛广运蒋善群李志平刘学文育峰唐根富王滨燕徐希平
Owner ANHUI PROV INST OF BIOLOGICAL MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com