Preparation method of Tetrandrine and Fangchino-kine

The technology of tetrandrine B and tetrandrine A is applied in the field of extracting effective parts of plants, which can solve the problems of complex process and high production cost, and achieve the effects of simple public art process, low production cost and cost reduction.

Inactive Publication Date: 2007-01-31
XIAN HAO TIAN BIO ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention overcomes the problems of com

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for extracting and separating tetrandrine and tetrandrine, comprising the following steps successively:

[0029] (1) Pulverization: Get 600kg of raw materials and pulverize them through a 16mm sieve to obtain 595kg of pulverized material. The content of raw materials is: 0.95% of tetrandrine and 0.495% of tetrandrine.

[0030] (2) Extraction: Take 500kg of pulverized material, put it into the extraction tank, and gradually add 40kg of lime powder along with the raw materials, then add 2000kg of methanol with a concentration of 70%, heat reflux extraction for 3 times, 1.5h / time, and combine the extract 5520kg , the extract was distilled under reduced pressure and concentrated to 260kg, placed at room temperature, filtered after 12 hours to obtain 40kg of precipitate, and dried to obtain 18kg of crude product.

[0031] Detected by high-pressure liquid phase (HPLC): the extract contains 5.24kg of tetrandrine and 2.73kg of tetrandrine, and the calculated extractio...

Embodiment 2

[0040] (1) Pulverization: Get 600kg of raw materials and pulverize them through a 16mm sieve to obtain 590kg of pulverized material. The content of the raw materials is: 0.95% of tetrandrine and 0.495% of tetrandrine.

[0041] (2) Extraction: Take 500kg of pulverized material, put it into the extraction tank, and gradually add 40kg of lime powder with the raw materials, then add 2000kg of ethanol with a concentration of 70%, heat reflux extraction for 3 times, 1.5h / time, and combine the extract 5650kg , the extract was concentrated to 265kg by distillation under reduced pressure, placed at room temperature, filtered to obtain 44kg of precipitate after 12 hours, and dried to obtain 20kg of crude product.

[0042] Detected by high-pressure liquid phase (HPLC): contain 5.42kg of tetrandrine and 2.97kg of tetrandrine in the extract, and the calculated extraction rate is 95%; B element 2.60kg, calculated precipitation effective component recovery rate is respectively 91% and 87.5%...

Embodiment 3

[0051] (1) Pulverization: Get 600kg of raw materials and pulverize them through a 16mm sieve to obtain 590kg of pulverized material. The content of raw materials is: 0.95% of tetrandrine and 0.495% of tetrandrine.

[0052] (2) Extraction: Take 500kg of crushed material, put it into the extraction tank, and gradually add 50kg of lime powder with the raw materials, then add 1500kg of methanol with a concentration of 80%, heat reflux extraction for 3 times, 2h / time, and combine the extraction solution with 4000kg for extraction The extract was distilled under reduced pressure and concentrated to 270kg, placed at room temperature, filtered after 12 hours to obtain 41kg of precipitate, and dried to obtain 18.5kg of crude product.

[0053] Detected by high pressure liquid phase (HPLC): the extract contains 5.30kg of tetrandrine and 2.70kg of tetrandrine, and the calculated extraction rate is 92.1%; the dried crude product contains 4.89kg of tetrandrine, and B element 2.40kg, calcul...

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Abstract

The present invention belongs to the field of plant effective component extraction technology. In particular, it relates to a preparation method of hanfangchin A and hanfangchin B. Said method includes the following steps: (1), preparing material: pulverizing or slicing raw material; (2), extraction: adding quicklime into raw material, added amount is 6-10% of raw material weight, then using polar organic solvent to make thermal reflux extraction of extract, recovering solvent, making concentrate undergo the processes of standing still, crystallization and filtration so as to obtain precipitate; (3), crystallization: using acetone to dissolve precipitate with thermal reflux, concentrating the dissolved solution to proper extent, standing still and crystallizing; (4), crude product preparation: using cold benzene to dissolve crystal to obtain insoluble substance and benzene dissolved solution, said insoluble substance is hanfangchin B crude product, the concentrated paste to benzene dissolved solution is hanfangchin A crude product.

Description

Technical field: [0001] The invention relates to the technical field of extracting effective parts of plants, in particular to a preparation method of tetrandrine and tetrandrine. Background technique: [0002] Fangji powder, a traditional Chinese medicine, has been used in China for thousands of years. Fenfangji (also known as Fangji) is the dried root slice of Stephania tetrandra S. Moore in the family Fangjiaceae. Alkaline (tetrandrine), tetrandrine base (tetrandrine, fangehinoline), tetrandrine, in addition to flavonoid glycosides, phenols, organic acids, volatile oils, etc. Studies have shown that tetrandrine and tetrandrine have analgesic, anti-inflammatory, blood pressure-lowering, anti-tumor, and striated muscle relaxation effects. In recent years, studies have found that tetrandrine also has anti-arrhythmic effects. At present, the method for extracting tetrandrine and fangehinoline from Tetendrex root mostly adopts the following process: after alcohol extraction, ...

Claims

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Application Information

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IPC IPC(8): C07D491/153C07D491/16A61K36/59
Inventor 胡洪来
Owner XIAN HAO TIAN BIO ENG TECH
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