Method of auxiliary screening cold resistant paddy rice and its special primer

A rice and cold-resistant technology, applied in the field of auxiliary screening of cold-resistant rice, can solve problems such as genetic bottlenecks, and achieve the effects of simple operation, shortening the breeding cycle, and speeding up the breeding speed.

Inactive Publication Date: 2007-01-31
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, the number of alleles in cultivated rice is about 60% of wild rice (Sun C Q, Wang X K, Li Z C, Yoshimura A. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers.Theor Appl Genet, 2001, 102: 157-162), which leads to the genetic bottleneck (genetic bottleneck) problem faced by the current rice variety breeding

Method used

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  • Method of auxiliary screening cold resistant paddy rice and its special primer
  • Method of auxiliary screening cold resistant paddy rice and its special primer
  • Method of auxiliary screening cold resistant paddy rice and its special primer

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1, Discovery of special primers and molecular markers

[0020] First, Jiangxi Dongxiang common wild rice was backcrossed and self-crossed with the ultra-high-yield variety Guichao 2, and a high-generation backcross population (BC 4 f 2 group), and identify the cold tolerance of this group in bud stage. The specific identification method is: soak the rice seeds with 5% sodium hypochlorite for 20 minutes, then rinse with water for 3-4 times, then soak the seeds at 37°C for 1 day, then place the seeds on the filter paper soaked in water in a glass test tube, put the test tube into Germinate in the light culture room (28°C day / 25°C night, 12h light and 12h dark every day, 83% relative humidity), and when the buds grow to about 5mm, select 100 robust and consistent buds and place them in a glass with a diameter of 4cm and a height of 9.5cm in a test tube. Put the test tube in a refrigerator at 4-5°C for 5 days at low temperature, then move the young shoots to the ...

Embodiment 2

[0026] Example 2, the amplification products (molecular markers) of p8-3, p12, p16, p18, p21, p22 and p32 in IL112 and Jiangxi Dongxiang common wild rice are related to the cold tolerance of the population

[0027] First, according to the method of Example 1, the F strain constructed by IL112 and Guichao 2 was backcrossed and self-crossed. 2∶3 The cold tolerance of the budding stage of the population was identified, and the live seedling rate of each strain was counted; at the same time, each F 2 Genomic DNA of single plant, carry out PCR amplification with the amplified product (molecular marker) of primer p8-3, p12, p16, p18, p21, p22 and p32 in IL112 and Jiangxi Dongxiang common wild rice in embodiment 1 as Marker Increase (PCR amplification reaction and electrophoresis condition are the same as embodiment 1) detect the band type of each strain, analyze genotype, analyze the relation of genotype and bud stage cold tolerance with T-Test method, the result finds: p8-3, p12 T...

Embodiment 3

[0030] Example 3, Feasibility verification of screening cold-tolerant rice with primer p21

[0031] Rice varieties (the following rice varieties except IL112 are all from the National Seed Resource Bank): IL112, 746, Yunfeng 7, Xiangjingnuo, Wan 88-1, Shangzhou 10, Miryang 111, Hexi 35, REIMEL, IR66746-76-3-2, 9505-138, Guichao 2, 03A-11, 03A-9, Zhongyou 13, 37760, Shuiyuan 349, Shuiyuan 332, Shuiyuan 287, VISTA, TP34 and SR64446-1.

[0032] At first by the method in embodiment 1, 20 rice varieties are carried out bud stage cold tolerance appraisal, appraisal result is as table 3:

[0033] rice variety

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Abstract

The present invention discloses a method for auxiliary screening cold-resistant rice and its special-purpose primer. Said primer is a pair of primers formed from nucleotide sequence of sequence 1 and nucleotide sequence of sequence 2 in sequence table. Said auxiliary screening method of cold-resistant rice includes the following steps: using genome DNA of rice to be detected as template, and using the above-mentioned a pair of primers to make PCR amplification, if in the amplification product of the detected rice the strip band whose size is 250-750 bp is existed, said detected rice is the cold-resistant rice.

Description

technical field [0001] The invention relates to a method for assisting in screening cold-tolerant rice and special primers thereof. Background technique [0002] Rice is an important food crop, and chilling injury at the bud stage is one of the important limiting factors affecting rice production in the early rice planting areas in the middle and lower reaches of the Yangtze River, the northeast and northwest rice areas, and the one-crop rice areas in the Yunnan-Guizhou Plateau. Chilling damage during the budding stage will lead to slow growth of rice seedlings, reduced tillering, and even severe cases of large areas of stiff seedlings and dead seedlings, which will eventually lead to a significant reduction in rice yield. Therefore, it is urgent to breed cold-resistant varieties of rice in the budding stage. . Common wild rice is the ancestor of cultivated rice in Asia. During the evolution of wild rice into cultivated rice, through natural selection and artificial selecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12P19/34C12N15/11
Inventor 孙传清刘凤霞谭禄宾苏震朱作峰付永彩
Owner CHINA AGRI UNIV
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