Reagent stripe for detecting alcohol content in saliva and box kit

A reagent strip and content technology, applied in the field of reagent strips and kits for detecting ethanol content in saliva, can solve the problems of unstable reagent strip activity, high price, short storage time, etc., to avoid infection and cross-sensitivity, and to be easy to use and save, the effect of fast response

Inactive Publication Date: 2007-01-31
上海赫赫信息科技有限公司
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AI Technical Summary

Problems solved by technology

For example, GM/MS and other instruments detect ethanol components. Although these methods can be accurately quantified, they have the disadvantages of expensive equipment, high detection costs, special training, and inability to quickly detect at the scene of the crime.
Breath alcohol analyzers can be used on-site, but they are also very expensive, unhygienic, and prone to cross-infecti

Method used

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  • Reagent stripe for detecting alcohol content in saliva and box kit
  • Reagent stripe for detecting alcohol content in saliva and box kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Preparation of detection reagent strips

[0032] ① Preparation of alcohol oxidase and horseradish peroxidase solution

[0033] Take 10 mg of alginate, 500 units of ethanol oxidase, 2500 units of horseradish peroxide, and 200 mg of gelatin dissolved in 10 ml of 1M Tris-malonic acid buffer at pH 7.2, and then add 10 ml of double distilled water.

[0034] ② Preparation of TMB solution

[0035] Weigh 400mg of TMB hydrochloride and dissolve it in 20ml of double-distilled water, decolorize with 0.1ml of 10mg / ml tryptophan hydrochloride, then add 20ml of xylene, and neutralize the hydrochloric acid with NaOH. After mixing, TMB was dissolved in xylene, and the solution was separated after standing, and the aqueous solution was discarded. A TMB / xylene solution was obtained.

[0036] ③ Immobilization of enzyme and substrate

[0037] Immerse a 0.5 cm wide filter paper in the above enzyme mixture, take out the filter paper and dry it for 20 minutes under hot air a...

Embodiment 2

[0041] Embodiment 2: On-site detection results of reagent strips

[0042] 1. Comparison of the blood detection data between the saliva ethanol detection reagent strip and the gas chromatography headspace method:

[0043] Distributed reagent strips were promoted and applied in 4 traffic police detachments in Shanghai, and on-site tests were carried out. The results of all tests were compared with gas chromatography headspace method (GC / MS) detection. The results showed that the detection results of the saliva ethanol detection reagent strips were basically consistent with those detected by the gas chromatography headspace method in blood. The accuracy rate is 98.0%.

[0044] 2. Sample collection method:

[0045] 1) Put the test strip directly into the mouth, wait for 10 seconds, take it out, and observe the color change; or

[0046] 2) Spit the saliva into the test vessel.

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Abstract

The invention discloses an agent tape and kit for testing alcohol content in spit. The content of alcohol in spit would be semi-quantification tested according to the color of agent tape after ethanol oxidase and peroxidase oxygenating N, N, N', N'-tetramethyl benzidine.

Description

technical field [0001] The invention relates to a reagent strip and a kit for detecting ethanol, in particular to a reagent strip and a kit for detecting ethanol content in saliva. Background technique [0002] In recent years, criminal cases caused by drinking and traffic accident cases caused by drunk driving have been on the rise. At present, many countries have formulated the relationship between blood ethanol concentration and traffic accident responsibility determination, and established a variety of methods that can detect blood ethanol content. For example, GM / MS and other instruments detect ethanol components. Although these methods can accurately quantify, they have the disadvantages of expensive equipment, high detection costs, special training, and inability to quickly detect at the scene of the crime. Breath ethanol analyzers can be used on-site, but they are also very expensive, unhygienic, and prone to cross-infection diseases. [0003] Therefore, a lot of d...

Claims

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Application Information

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IPC IPC(8): G01N33/98G01N33/52G01N21/78
Inventor 吴献斌胡小龙王学生黄建东张艳顾琳周华山
Owner 上海赫赫信息科技有限公司
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