Method of preparing high activity immobilization penicillin acylase hybridization carrier

Abstract

the invention discloses a hybridized carrier preparing method of high-activity solidified penicillin acylating enzyme, which comprises the following steps: blending cyclohexane, polyethylene glycol octylphenyl ether and positive hexyl alcohol evenly; adding ammonia; allocating transparent inverse-phase micro-emulsion; stirring for 15 min; adding silicic carbethoxy to stir for 30 min; adding siliconiting agent; setting the bulk rate of silicic carbethoxy and siliconiting agent at 0.5-9.5: 0.5-9.5; hydrolyzing; condensing for 22h; washing through acetone; drying in the vacuum at indoor temperature to obtain the product.

Description

technical field [0001] The invention relates to a method for preparing a catalyst carrier, in particular to a method for preparing a highly active immobilized penicillin acylase hybrid carrier. Background technique [0002] Enzyme is a biological catalyst whose chemical essence is protein. Because of its high catalytic activity, strong selective specificity and mild reaction conditions, enzymes have been widely used in biochemical, pharmaceutical and chemical industries, food industry, light industry and other fields. At present, thousands of enzymes have been discovered. Although water-soluble enzymes have many advantages that other catalysts cannot match, because water-soluble enzymes are produced according to the needs of organisms themselves, they are greatly limited in practical application. The main disadvantages are: [0003] (1) The extraction and purification are complicated and expensive; [0004] (2) After the reaction, recycling and reuse has certain difficult...

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Problems solved by technology

[0003] (1) The extraction and purification are complicated and expensive;

[0004] (2) After the reaction, recycling and reuse has certain difficulties technically;

[0005] (3) Difficulty in product separation and purification, which increases production costs;

[0006] (4) Cannot be used in organic solvents, strong acid, strong alkali or high temperature, the stability is poor, and some enzymes will lose their activity when used under mild conditions

[0009] (2) It is very easy to separate the enzyme from the substrate and product;

CN 1279287A invented a multi-component copolymer porous microbead as a carrier for immobilizing penicillin acylase, but the structure and properties of the polymer itself determine that it has the following weaknesses: control the pore structure of the polymer and the presence of functional groups on the surface The quantity and its distribution are very difficult; some functional groups are in the highly cross-linked network of polymers. Although the situation has been improved after the solvent is swollen, it may still be difficult for the reactant molecules to approach, thus reducing the surface of the carrier. Utilization rate; thermal stability, mechanical stability and solvent resistance of polymer carrier are weak; it is difficult to dispose of polymer carrier after waste

Aiming at the weakness of polymer carriers, people have developed and used some inorganic carriers, such as CN 1320688A, which uses MCM-41 as a carrier for immobilizing penicillin acylase, but the activity of the immobilized enzyme prepared is not high, and it is mainly combined by adsorption. Therefore, the operational stability of the immobilized enzyme is not good.

In order to improve the stability of the immobilized enzyme, the surface of the inorganic carrier is usually modified and functional groups are grafted, but the carrier modified by this method has many disadvantages such as limited number of functional groups and uneven distribution.

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