Method for extracting superoxide dismutase from blood of cattle, sheep

A technology of superoxide and dismutase, which is applied in the field of superoxide dismutase extraction, can solve the problems of reduced product yield, long production cycle, complex process, etc., and achieve short production cycle, low cost and simple production process Effect

Inactive Publication Date: 2007-02-21
云南呈贡光明亚南经贸有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the process of this method is more complicated and the production cycle is long; due to the use of expensive column chromatography system, column packing and high-speed centrifuge, the cost is high, and after column chromatography, the yield of the product is greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 of the present invention: the method for extracting superoxide dismutase from bovine blood

[0015] (1), add 20g of sodium citrate anticoagulant to 10kg of fresh bovine blood, place it at a low temperature of 6°C for 80 minutes, wait for the solution to be layered, and remove most of the supernatant to obtain 8500ml of precipitation;

[0016] (2), the above-mentioned precipitate is rapidly heated from room temperature to 50° C., and after being incubated for 15 minutes, it is rapidly lowered to room temperature, and the precipitate is removed after centrifugation at 4000 rpm for 15 minutes to obtain 5000 ml of supernatant;

[0017] (3), the supernatant obtained in step (2) is subjected to 3-level temperature gradient treatment: the 5000ml supernatant obtained in step (2) is rapidly warmed up to 40° C. from room temperature, and then rapidly lowered to room temperature after being kept for 20 minutes. Centrifuge at 4000 rpm for 10 minutes to remove the preci...

Embodiment 2

[0020] Embodiment 2 of the present invention: the method for extracting superoxide dismutase from bovine blood

[0021] (1), add 30g of sodium citrate anticoagulant to 10kg of fresh bovine blood, place it at a low temperature of 4°C for 30 minutes, wait for the solution to be layered, and remove most of the supernatant to obtain 8500ml of precipitation;

[0022] (2), the above-mentioned precipitate is rapidly heated from room temperature to 40° C., and after being incubated for 10 minutes, it is rapidly lowered to room temperature, and the precipitate is removed after centrifugation at 4000 rpm for 30 minutes to obtain 5100 ml of supernatant;

[0023] (3) The supernatant obtained in step (2) is subjected to a 4-level temperature gradient treatment: the 5100ml supernatant obtained in step (2) is rapidly warmed up from room temperature to 40° C., kept for 10 minutes and then rapidly lowered to room temperature, 4000 Centrifuge at rpm for 10 minutes to remove the precipitate to o...

Embodiment 3

[0026] Embodiment 3 of the present invention: the method for extracting superoxide dismutase from bovine blood

[0027] (1), add 50g of sodium citrate anticoagulant to 10kg of fresh bovine blood, place it at a low temperature of 8°C for 100 minutes, wait for the solution to be layered, and remove most of the supernatant to obtain 8500ml of precipitation;

[0028] (2), the above-mentioned precipitate is rapidly heated from room temperature to 50° C., and after being incubated for 30 minutes, it is rapidly lowered to room temperature, and the precipitate is removed after centrifugation at 6000 rpm for 10 minutes to obtain 5300 ml of supernatant;

[0029](3) The supernatant obtained in step (2) is subjected to 6-level temperature gradient treatment: the 5300ml supernatant obtained in step (2) is rapidly heated from room temperature to 50°C, and then rapidly cooled to room temperature after being kept for 10 minutes, 4000 After centrifuging for 30 minutes per minute, the precipita...

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Abstract

This invention discloses a method for extracting superoxide dismutase from cow and sheep blood. The method comprises: subjecting fresh cow and sheep blood to anti-coagulation, precipitation, 3-6 stages of temperature gradient treatment, salting out, ultrafiltration, concentration and freeze-drying to obtain two groups of superoxide dismutase products with slightly different specific activity and purity. Compared with present techniques, the method has such advantages as simple process, short production period, high yield, and high product specific activity, and stable product quality, low cost and high profit.

Description

Technical field: [0001] The invention relates to a method for extracting superoxide dismutase (SOD), in particular to a method for extracting superoxide dismutase from blood of cattle and sheep. Background technique: [0002] Superoxide dismutase (Superoxide dismutase, abbreviated as SOD) is a metalloenzyme that can catalyze the dismutation reaction of superoxide anion free radicals. Function, widely used in medicine, food, cosmetics and other industries. At present, the method for extracting superoxide dismutase from cattle and sheep blood is usually to get fresh cattle and sheep blood to collect red blood cells by centrifugation, stir vigorously to make it hemolyze, add ammonium sulfate after hemolysis and carry out salting out, and collect 50% to 90% of the precipitation, use a high-speed centrifuge to remove most of the impurity proteins, and then use organic solvents such as chloroform, ethanol, acetone to precipitate, further remove the impurity proteins, go through s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 谭亚兰李蒙
Owner 云南呈贡光明亚南经贸有限责任公司
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