Bacillus subtilis, bacterium agent and application thereof

A technology of Bacillus subtilis and microbial inoculum, which is applied in the field of microbial inoculum, and can solve problems such as easy loss of disease resistance, single disease-resistant varieties, and environmental pollution

Inactive Publication Date: 2007-03-07
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] One of the objectives of the present invention is to provide a kind of subtilis with high efficiency and broad-spectrum bactericidal activity that can be used for biological control

Method used

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  • Bacillus subtilis, bacterium agent and application thereof
  • Bacillus subtilis, bacterium agent and application thereof
  • Bacillus subtilis, bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of Corn-1 microbial inoculum is carried out according to the following steps:

[0053] (1) Strain activation: Activate the strain Corn-1 stored at -40°C on the LB plate medium (30°C), pick a single colony and multiply it on the LB slant medium for later use;

[0054] (2) Preparation of seed liquid: make LB liquid culture medium according to the conventional method, put 200 mL of LB culture liquid in a 500 mL Erlenmeyer flask, and sterilize with high-pressure damp heat. After the temperature drops to room temperature, insert step (1) into each bottle. An inoculation loop of the above-mentioned activated Corn-1 strain was carried out on a shaker for shaking culture at a rotation speed of 170 rpm, and cultured at 30°C for 22 hours, and the obtained can be used as a seed solution;

[0055] (3) Preparation of sucrose bean cake medium: in a 500L fermenter, add 10.5kg of sucrose, 7kg of bean cake powder, 0.35kg of NaCl, CaCO 3 0.35kg, KH 2 PO 4 0.07kg and ...

Embodiment 2

[0059] The experiment was carried out in the greenhouse of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Curvularia spp. Liao 9605, No. 1 race of maize leaf spot pathogen and 2010-1 maize leaf spot pathogen were selected as pathogenic bacteria respectively. The pathogenic bacteria are all dominant races of corn leaf spot in the Huang-Huai-Hai area, provided by the Comprehensive Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences; the susceptible variety 9831 was selected for corn small spot and Curvularia leaf spot, and the corn big spot The susceptible variety 8112 was used in the experiment, all provided by the Comprehensive Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Seedlings were raised in pots, 10 seedlings per pot, and each treatment was repeated four times. The bacillus subtilis Corn-1 microbial agent of embod...

Embodiment 3

[0063] The experiment was carried out in the experimental field of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, with two rows per plot, four repetitions, and random block arrangement. Adopt the bacillus subtilis Corn-1 bacterium agent (diluted with water containing thalline 10) prepared by pathogenic bacteria and embodiment 1 using knapsack type manual sprayer 8 cfu / mL); set up control agent (45% dysonium SL, commercially available, produced by Hebei Shuangji Huagong Co., Ltd., 1500 times water dilution liquid spray treatment) and blank control (clear water spray); processing method is the same as embodiment 2 , Watered enough after inoculation of pathogenic bacteria. After the blank control is fully onset, investigate the disease grade (same as Example 2), and calculate the disease index and preventive effect. The test results showed (see Table 3) that the disease indexes of the three corn leaf spot diseases after the bacterial agent...

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Abstract

The invention discloses a Bacillius subtilis Corn-1, which is reserved in the Chinese microbe bacterial committee center with reserving number at CGMCC No.1778.

Description

technical field [0001] The invention relates to a bacillus subtilis, and also relates to a microbial agent produced by using the bacillus subtilis, a preparation method and an application. Background technique [0002] Corn leaf spot diseases include corn leaf spot, corn leaf spot and corn small spot. [0003] Corn Curvularia leaf spot, also known as pseudo eye spot and black mold, is a dangerous corn disease caused by Curvularia lunata. The disease mainly damages the leaves and leaf sheaths, and the diseased spots are densely distributed on the leaves of severely diseased leaves, causing the leaves to scorch. In the mid-1980s, the disease was discovered in Xinxiang, Henan Province, my country. The disease develops and spreads rapidly, and has commonly occurred in northern corn producing areas such as Liaoning, Beijing, Hebei, Henan, Shandong, Jilin, Tianjin, etc. Another important disease of maize poses a great threat to corn production. Such as 1994, Hebei Province, Lo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12R1/125
Inventor 马平李社增孔令晓鹿秀云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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