Nanometer NAMI-A particle and its prepn process and application
A NAMI-A, nanoparticle technology, applied in antitumor drugs, drug combinations, powder delivery, etc., can solve the problems of fast metabolism, lack of selectivity, enhanced drug side effects, etc., achieving good biocompatibility and simple preparation method , good anticancer activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0025] Dissolve 150mg of PLGA (molecular weight: 8000) in 4ml of dichloromethane, add 6ml of an aqueous solution containing 20mg of NAMI-A into the PLGA solution, and ultrasonically emulsify for 5min with an ultrasonic cell disruptor. The formed suspension was added to 20 ml of an aqueous solution in which 100 mg of sodium cholate was dissolved, and ultrasonically emulsified for 5 min to obtain a suspension. The above suspension was passed through a microporous filter membrane with a pore size of 0.12 μm, and the filtrate was centrifuged (15000 rpm) for 15 min in a high-speed centrifuge to collect the solid. The collected solid was dispersed with distilled water, and centrifuged once more (15000 rpm) to collect the solid. The collected solid was freeze-dried for 24 hours with a freeze dryer to obtain NAMI-A nanoparticles in the form of white powder. The particle diameter measured by a laser particle size analyzer is 195nm; the particle is a regular spherical shape as observed...
Embodiment 2
[0027] 100 mg of PLGA (molecular weight: 8000) was dissolved in 4 ml of dichloromethane, 6 ml of an aqueous solution dissolved with 10 mg of NAMI-A was added to the PLGA solution, and ultrasonically emulsified for 10 min. The formed suspension was added to 20 ml of an aqueous solution in which 150 mg of sodium cholate was dissolved, and ultrasonically emulsified for 10 min to obtain a suspension. The above suspension was passed through a microporous filter membrane with a pore size of 0.12 μm, and the filtrate was centrifuged (15000 rpm) for 15 min in a high-speed centrifuge to collect the solid. The collected solid was dispersed with distilled water, and centrifuged once more (15000 rpm) to collect the solid. The collected solid was freeze-dried for 24 hours with a freeze dryer to obtain NAMI-A nanoparticles in the form of white powder. The particle diameter measured by a laser particle size analyzer is 133nm; the particle is a regular spherical shape observed by a thermal f...
Embodiment 3
[0029] Dissolve 100mg chitosan in 50ml 1% acetic acid solution and pass through a 0.22μm filter membrane. 10mg NAMI-A is dissolved in the chitosan solution, under stirring at room temperature (200rpm), the above solution is added dropwise into 20ml of the TPP aqueous solution with a concentration of 1.0mg / ml with a No. 7 syringe, so that the mass of chitosan and TPP The ratio was 5:1, and stirred for 10 minutes. Centrifuge at high speed (12000rpm) for 10min to collect the solid. The collected solid was dispersed with distilled water, and centrifuged once more (15000 rpm) to collect the solid. Vacuum-dried at room temperature to obtain white powdery NAMI-A nanoparticles. The particle is observed by the thermal field and the scanning electron microscope as a regular spherical shape, and the particle diameter is about 50nm.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More