Method for improving milk lactoprotein content by use of processing protein feedstuff
A technology of protein feed and content, which is applied in the field of animal nutrition and feed science of animal husbandry, can solve the problems of reducing the rumen pass rate of high-quality protein feed, cannot be popularized and used, and the effect is not ideal, so as to improve the three-dimensional pollution, increase the utilization rate, The effect of improving production performance
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[0037] Example 1 Screening example of protective protein feed method
[0038] The protection method of rumen-passing protein feed is to spray 0.5%-8% polyhydroxy ketone (or aldehyde) (xylose in this example) solution into the raw material (soybean meal in this example), after fully mixing, at a certain 60 Heating at ℃-140℃ for 20-120 minutes to obtain rumen-protected protein feeds with different treatments. Table 1 is a brief list of some processing groups completed by the present group under laboratory conditions.
[0039] Table 1: Brief list of different treatment groups produced by composite machining process
[0040] Note: The above treatment groups are expressed in the form of "number / number / number". For example: the combination 105 / 8 / 120 is the treatment group added with 8% xylose at 105°C and heated for 120min. It is still represented in this way in the following examples.
[0041] The parameters of D-xylose addition 3%, temperature 90°C, and time 90min in Example (...
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[0042] Example 2 Determination of crude protein degradation rate of treated soybean meal by single pepsin enzymolysis method
[0043] Weigh 0.5 g of the protected soybean meal obtained in Example 1 into a 100-ml conical flask, add 30 ml of an enzyme solution containing 120 IU of pepsin with a pH of 1.55, and incubate with a water bath shaker for 24 hours at 40°C and 90 rpt / min. After taking out, add 5ml of 20% SAA (sulfosalicylic acid) to each conical flask to precipitate the undigested protein. After standing for 30min, filter with medium-speed filter paper, and then rinse the residue with preheated 1% SAA. . Transfer the residue together with the filter paper into a weighing bottle, and bake it in an oven at 60°C for 24h to constant weight. Then the protein content was determined by Kjeldahl method and its dry matter disappearance rate and protein degradation rate were calculated, as shown in Table 2.
[0044] Table 2 The protein degradation rate of soybean meal after trea...
Example Embodiment
[0046] Example 3 Determination of crude protein degradation rate of treated soybean meal by pepsin-trypsin two-step enzymolysis method
[0047] Weigh about 0.5 g of the protected soybean meal obtained in Example 1 into a 100-ml conical flask, add 30 ml of an enzyme solution containing 120 IU of pepsin and a pH of 1.55. The pH of the digestion solution was adjusted to 7.8, 5 ml of an enzyme solution containing 160 mg of trypsin with a pH of 7.8 was added, and the culture was continued at 39° C. for 18 h. After taking out, add 5ml of 20% SAA (sulfosalicylic acid) to each conical flask to precipitate the undigested protein. After standing for 30min, filter with medium-speed filter paper, and then use preheated 1% SAA to sanitize the residue. rinse. Transfer the residue together with the filter paper into a weighing bottle, and bake it in an oven at 60°C for 24h to constant weight. Then, the protein content was determined by Kjeldahl method, and the dry matter disappearance rate...
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