74 results about "Process protein" patented technology

Compositions and methods for improving expression and / or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

Compositions and methods for improving expression and / or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.

A melt-processed protein composition formed from a protein, plasticizer, and an electrophilic reagent is provided. The electrophilic reagent, for instance, may be selected to undergo a nucleophilic addition reaction with free sulfhydryl and / or thiyl radicals to help minimize the formation of disulfide crosslinking bonds that could otherwise lead to protein aggregation during melt processing. To enhance the degree to which the electrophilic reagent can limit crosslinking, a plasticizer is also employed that helps to mediate the adsorption of the electrophilic reagent into the internal structure of the protein, where it can be more stably retained. Furthermore, the temperature and shear rate employed during melt blending may also be selected to be relatively low to help limit polypeptide dissociation, thereby minimizing the impact of aggregation and embrittlement.

The use of gellan gum in combination with carboxymethyl cellulose (cellulose gum) in acidified protein beverages is described. Acifidied protein beverages comprising a combination of cellulose gum and gellan gum and methods to prepare these beverages are also described.

The invention provides human protein complexes with endonuclease activity. In particular, the invention provides human protein complexes with tRNA splicing endonuclease activity and / or 3′ end pre-mRNA endonuclease activity. The invention also provides a splice variant of human Sen2, namely human Sen2deltaEx8, and human protein complexes comprising human Sen2deltaEx8. The human Sen2deltaEx8 complexes have pre-tRNA cleavage activity and / or 3′ end pre-mRNA endonuclease activity. The invention also provides human protein complexes with pre-ribosomal RNA cleavage activity. The invention also provides antibodies that immunospecifically bind to a complex described herein or a component thereof, and methods of diagnosing, preventing, treating, managing or ameliorating a disorder utilizing such antibodies. The present invention also provides methods utilizing the complexes described herein, inter alia, in screening, diagnosis, and therapy. The invention further provides methods of preparing and purifying the complexes. The present invention further provides methods of identifying a compound that modulates the expression of a component of a complex described herein, the formation of a complex described herein or the activity of a complex described herein, and methods of preventing, treating, managing or ameliorating a disorder, such as a proliferative disorder, or a symptom thereof utilizing a compound identified in accordance with the methods.

The invention provides human protein complexes with endonuclease activity. In particular, the invention provides human protein complexes with tRNA splicing endonuclease activity and / or 3' end pre-mRNA endonuclease activity. The invention also provides a splice variant of human Sen2, namely human Sen2deltaEx8, and human protein complexes comprising human Sen2deltaEx8. The human Sen2deltaEx8 complexes have pre-tRNA cleavage activity and / or 3' end pre-mRNA endonuclease activity. The invention also provides human protein complexes with pre-ribosomal RNA cleavage activity. The invention also provides antibodies that immunospecifically bind to a complex described herein or a component thereof, and methods of diagnosing, preventing, treating, managing or ameliorating a disorder utilizing such antibodies. The present invention also provides methods utilizing the complexes described herein, inter alia, in screening, diagnosis, and therapy. The invention further provides methods of preparing and purifying the complexes. The present invention further provides methods of identifying a compound that modulates the expression of a component of a complex described herein, the formation of a complex described herein or the activity of a complex described herein, and methods of preventing, treating, managing or ameliorating a disorder, such as a proliferative disorder, or a symptom thereof utilizing a compound identified in accordance with the methods.

A novel protein allergen Der p VII of Dennatophagoides pteronyssinus is described. A cDNA clone encoding Der p VII was isolated from a lambda gt11 library of D. pteronyssinus cDNA. The nucleic acid sequence of der p VII encodes a 198 residue mature processed protein having a predicted molecular weight of 22,177 daltons. Der p VII protein may be used as the active ingredient in therapeutic composition for the treatment of sensitivity to house dust mites. The protein may also be used in methods of diagnosing such sensitivity.

The invention provides a method for preparing biological adhesive by using perna viridis to adhere protein, relates to a biological adhesive. The invention provides a method for preparing a waterproof biological adhesive with better biological compatibility and low cost that extracts adhesive protein from perna viridis having wide raw material and low cost. The method includes steps as follows: cutting foot from live perna viridis, then mincing and homogenating, processing protein acidification, acetone protein precipitation, purification, finally, obtaining the waterproof biological adhesive. The adhesive protein waterproof biological adhesive can be obtained rapidly from perna viridis most abundant in China southeast seaside. The perna viridis biological adhesive has characteristics of high production, low cost and better adhesive effect. The prepared adhesive can adhere on surface of glass, metallic titanium and plastic, adheres cell in short time, adheres plastic or mouse thighbone, and has no cell toxicity. The biological adhesive as biological medical material biological adhesive has wide application prospect in clinical use.

The invention provides broiler concentrated feed, which is prepared by processing protein feed, trace element premix, vitamin premix, mineral premix and feed toxin adsorbent. The feed comprises the following components in percentage by weight: 45.7 percent of 46% puffed bean pulp, 15.00 percent of cottonseed meal, 12.00 percent of rapeseed meal, 6.00 percent of hydrolyzed feather meal, 5.20 percent of chili meal, 3.33 percent of calcium hydrophosphate, 4.55 percent of mountain flour, 0.84 percent of edible salt, 3.00 percent of specific core feed for broiler concentrated feed, 3.00 percent of soybean oil, 0.35 percent of 50 percent choline, 0.5 percent of anhydrous sodium sulphate, 0.05 percent of phytase type 5000, and 0.03 percent of antioxidant. Due to the content of amino acid and sedimentation of natural pigment of natural chili meal raw material, the content of various amino acids is completely in accordance with animal maintenance and production requirement, the feed conversion is large, and nutrient output is reduced to minimum, so that digestion and absorption can be facilitated, the palatability is good, and the cost is low.

The invention discloses a technology for processing protein feed by insects The technology comprises the following steps of: culturing zophobas morio progenitive eggs in a microbiological feed; sterilizing pure insects at a high temperature, and milling into slurry; adding neutral protease in the slurry, sealing, hydrolyzing for 46h-50h, controlling the temperature at 48 DEG C to 52 DEG C; performing high-temperature spray drying to the hydrolyzed slurry, in order to form pure insect slurry protein powders; mixing the pure insect slurry protein powders with the crushed microbiological feed, stirring uniformly, and preparing biological feed by bug peptide protein, so as to obtain the product. With the adoption of the technique, the bug peptide protein for preparing biological feed contains complete nutrients, is easy to digest and absorb, and is a high-quality animal protein capable of completely replacing fish powders; the great effects of enhancing body immunity and disease resistance, reducing disease, and reducing medicine cost are provided due to the rich antibacterial peptide and bacterial protein; with the adoption of the technique, the productivity of livestock is enhanced, and the meat quality and taste are improved.

A novel high-protein, reduced carbohydrate food material technology, and high-protein, reduced carbohydrate food products made therefrom, in which the food products meet high organoleptic, stability, and taste / texture standards. This novel material technology possesses numerous controllable functional characteristics, including high to low adhesion, high to low volume expansion, high to low tensile strength, and high to low break elongation, all of which are critical to both processing needs as well as final food product specifications. The material technology allows for the processing of proteinaceous foods on common process equipment, the foods including but not limited to chips, snacks, crackers, wafers, bars, flat breads, cookies, biscuits, breads, bagels, cakes, waffles, pancakes, french fries, pasta, pizza dough, breakfast cereals, muffins, doughnuts, pastries, and meat analogs. The material is an edible dough that possesses the material characteristics necessary for numerous industrial food processes, including direct reduction sheeting, lamination sheeting, extrusion, die cutting, and rotary molding, followed by on or more of baking, drying, microwaving, boiling, steaming, frying, seasoning, and enrobing.

The invention discloses a process capable of removing heavy metals and used for processing protein powder from rice. The process comprises the following steps: (1) weighing 100 g of rice with excessive cadmium and adding 0.8 L of a sodium hydroxide solution with a mass concentration of 0.3 wt%; (2) adding 0.8 L of the sodium hydroxide solution with a mass concentration of 0.3 wt% into the rice treated in the step (1) carrying out crushing and wet grinding via a bead mill to obtain rice milk, and allowing the particle size of the rice milk obtained after grinding to be no more than 100 [mu]m; (3) conveying the rice milk obtained in the step (2) to a centrifuge and carrying out centrifugation at a speed of 2000 rpm for 9 min so as to separate rice starch and a rice protein solution; (4) adding 1 L of clear water into the rice starch obtained in the step (3), carrying out rinsing twice, then adding 350 ml of a citric acid solution with a pH value of 4.0 and carrying out stirring with a stirrer at a temperature of 35 DEG C and a speed of 1000 rpm for 4 h; (5) adding the rice starch and rice protein treated in the step (4) into an autoclave; and (6) drying the sterilized rice starch and rice protein in a baking oven with a temperature of 80 DEG C.

The invention discloses 214 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites / proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor / Scaffold proteins, Cytoskeleton proteins, GTP Signaling proteins, Kinases, Metabolism proteins, Phosphatases / Phospho-diesterases / Proteases, Receptor proteins, RNA Processing proteins, Transcription proteins, Translation proteins, Transporter proteins, and Ubitquitin proteins, as well as other protein types.

The invention discloses a method for processing protein powder through enzymolysis of fish soluble. Enzymolysis comprises chymotrypsin enzymolysis and flavourzyme enzymolysis. Infrared rays are adopted for temperature control in the enzymolysis process, a protease activating agent is added while chymotrypsin is added for enzymolysis, dialysis is performed after enzymolysis, a filtered solution is taken, fermented to remove a fishy smell and subjected to spray drying, and the protein powder is obtained. The method has the benefits as follows: chymotrypsin and flavourzyme are adopted for separate enzymolysis and are matched scientifically, the enzymolysis efficiency is high, and the molecular weight of the obtained protein is small; the infrared rays are adopted for temperature control, the enzymolysis time can be greatly reduced, the production cycle of the product is shortened, and the labor cost and the time cost are reduced. Fermentation is adopted to remove the fishy smell, the fishy smell can be removed, and the protein powder can have a special flavor; the protease activating agent is used for enzymolysis and can improve the activity of protease, the consumption of the protease is reduced, the enzymolysis time is shortened, and the production cost of the product is reduced.

The invention discloses 214 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites / proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor / Scaffold proteins, Cytoskeleton proteins, GTP Signaling proteins, Kinases, Metabolism proteins, Phosphatases / Phospho-diesterases / Proteases, Receptor proteins, RNA Processing proteins, Transcription proteins, Translation proteins, Transporter proteins, and Ubitquitin proteins, as well as other protein types.

The invention belongs to a production technology of dairy products, in particular to a method for applying an ultrafiltration technology to the proportion standardization of protein and fat in cheese processing. The invention uses an ultrafiltration membrane to concentrate whole milk or skim milk, remove part of whey and water, reduce the adding amount of the skim milk and improve the yield of the cheese. The concentrated milk protein content is 4 percent to 6 percent after ultrafiltration processing, and the concentrated milk protein is used for adjusting a proportion of the protein to the fat in raw milk. The method has the operation temperature of 4-10 DEG C and the ultrafiltration membrane operating pressure of 0.05 -0.08 MPa and improves the operating efficiency of the ultrafiltration membrane.

A melt-processed protein composition formed from a protein, plasticizer, and an electrophilic reagent is provided. The electrophilic reagent, for instance, may be selected to undergo a nucleophilic addition reaction with free sulfhydryl and / or thiyl radicals to help minimize the formation of disulfide crosslinking bonds that could otherwise lead to protein aggregation during melt processing. To enhance the degree to which the electrophilic reagent can limit crosslinking, a plasticizer is also employed that helps to mediate the adsorption of the electrophilic reagent into the internal structure of the protein, where it can be more stably retained. Furthermore, the temperature and shear rate employed during melt blending may also be selected to be relatively low to help limit polypeptide dissociation, thereby minimizing the impact of aggregation and embrittlement.

The invention relates to a novel pulp preparation device for producing rice protein (peptide) powder. At present, pulp preparation devices in the prior art are simple in structure and single in functions, and most of the devices are only provided with a pulp preparation function. When rice contains rice particles and shells with large volume, the rice particles and shells with large volume are difficult to dissolve in water, so that processed protein (peptide) powder is not pure. The invention relates to the novel pulp preparation device for producing rice protein (peptide) powder. The top ofa machine body is provided with a feeding bin; two sides inside the machine body are provided with material guiding blocks; the material guiding blocks are connected to the machine body; a feeding port is arranged between the two guiding blocks; a fixed grinding disc is arranged on the lower surface of each material guiding block; a movable grinding disc is arranged at the lower end of each fixedgrinding disc; and each movable grinding disc is movably connected to a supporting bearing. The device effectively combines a grinding process, a screening process and a pulp preparation process in the process of producing the protein (peptide) powder, so that the pulp preparation device is more comprehensive in functions, and labor cost and time cost in the turnover process between different processes are saved.

A method of determining and evaluating quality of peanut raw material suitable for processing protein. The method includes the following step: determining fruit shape score, total protein content, leucine content, arginine content, conarachin I content and the mass percentage of the subunit with molecular weight of 23.5 kDa to total protein in the peanut sample to be tested; putting the determined values into formula (1) to obtain the protein powder quality of the peanut sample. The disclosure reduces the analysis step. The disclosure establishes the model of evaluating raw material quality for peanut protein processing, and the peanut protein powder quality can be determined by 6 peanut quality characteristics. The determination of indexes in the model can be predicted by the near infrared analyzer. Through the near infrared analysis of peanut kernel, the indexes in the model can be simultaneously predicted without any damage to the peanut kernel.

The invention aims at providing a method for decoloration, debitterization and deodorization of fish protein liquid. Decoloration, debitterization and deodorization processing is performed on the protein liquid, protein loss can be reduced as much as possible, and nutrient ingredients of the protein liquid are reserved to the maximum extent. The method includes the following steps that resin I is soaked in an ethanol solution to be activated and then is washed with water till no ethanol flavor exists, and the washed resin I is packed for use; resin II is soaked in an HCl solution to be activated and then is washed with water till pH is 5-6, and the washed resin II is packed for use; the fish protein liquid flows through the resin I and resin II to perform adsorption after residues are filtered out. Permeated liquid is collected and is the fish protein liquid where decoloration, debitterization and deodorization are performed. The method has the advantages that operation is easy, the application range is wide and the method is efficient and economic, the processed protein liquid can achieve the targets of decoloration, debitterization and deodorization at a time, and the quality of the protein liquid is improved greatly.

The invention relates to a method for pre-processing protein foaming liquid by irradiation, comprising: 1) centrifugally separating protein liquid extracted from residual activated sludge so as to remove impurity therein, storing in a sealing device bottle; 2) evenly irradiating the sealing device bottle filled with protein foaming liquid by ray (60) Co, regulating the amount of irradiation agent in the range of 1KGy to 10KGy, and irradiating for 30min to 2h at room temperature; 3) spraying and drying the protein foaming liquid that is irradiated within 2h, obtaining the protein foaming agent. The irradiation technology is applied to the aspect of deodorizing protein, and can deodorize by sterilizing, and meanwhile, the effect of deodorizing is obvious by modifying volatile smelly material. The protein foaming agent prepared by the method is long in shelf life as long as 5 years; and the protein foaming agent can be biodegraded without environmental hidden trouble, thereby being capable of being used as high-quality foaming raw materials for preparing green and environment-friendly protein foam fire extinguishing agent and foam concrete.

The invention discloses a process for processing protein powder from corns, relating to the technical field of separation extraction and deep processing of natural protein. The process procedure comprises the steps of crushing and pulping of a raw material, enzymolysis, separation, protein powder drying and protein powder crushing; in the crushing and pulping process of raw materials, a certain amount of corns is soaked with warm water of 40 to 60 DEG C and is then crushed and pulped to 100 meshes under the ultrasonic assistance; in the enzymolysis process, pH value and enzymolysis temperature are adjusted, corn pulp is hydrated by an enzymatic process, the enzymolysis temperature is 40 to 60 DEG C, and pH value is weakly alkaline being 7.0 to 7.5. According to the method for extracting the protein powder from the corns, the corns are crushed and pulped under the ultrasonic assistance, so that the yield of the protein powder is increased. In the meantime, the process disclosed by the invention is relatively short in processing steps, simple and feasible, better in stability and repeatability, and free of strongly alkaline or toxic soaking agents, and thus being safe and free of pollution.

The invention relates to a protein extraction process, and in particular, relates to a method for processing proteins in potato starch production wastewater. The method can perform extraction treatment on cleaning wastewater, sieving washing water, potato washing water and other various waste yellow serofluid, the waste yellow serofluid extraction utilization rate is increased significantly, an acid is added in the yellow serofluid so as to make the pH value decreased, and then a compound agent a is added to make colloidal-state proteins in the yellow serofluid to form flocs to be precipitated; after static precipitation is carried out for 1 h, a compound agent b is added, and then static precipitation is carried out for 1 h, so that the separation effect can be improved, and protein precipitation is ensured. The extraction rate of separation and extraction of the proteins in the wastewater reaches 90% or more. Through detection, the waste yellow serofluid treated by the method has the chemical oxygen demand COD of 0.027% that of waste yellow serofluid with the same volume, and has the biochemical oxygen demand BOD5 of 0.041% that of the waste yellow serofluid with the same volume, so as to fully reach national second-class water discharge standards.

The invention relates to methods and devices used for digesting small amounts of protein in tiny cut gel pieces and for extracting the peptides resulting from the digestion in preparation for analysis by mass spectrometry. The invention involves digesting proteins using enzymes within the gel pieces in vessels which have permeable but lyophobic bases in such a manner that they scarcely touch the walls of the vessel, and then rapidly removing the digested proteins from the gel pieces almost completely by gentle centrifuging. It is then advantageous to bond the peptides reversibly to suitable surfaces as quickly as possible. For this purpose, the bases of the vessels may contain structures for bonding the peptides which are suitable for washing and subsequently eluting the peptides. A number of vessels can be combined together to form plates which, for example, can have the size of microtiter plates.

The invention discloses a technological process for processing protein powder by a low-temperature crushing machine and a thermostatic waterbath leaching method. A preparation method comprises the following specific steps of (1) selecting selenium-enriched peanuts, removing peanut shells, drying the peanuts without the shells, removing peanut coats so as to obtain peanut kernels; (2) pre-crushing the peanut kernels so as to obtain peanut powder; (3) performing ultra-high pressure auxiliary leaching on the peanut powder, and performing degreasing; (4) performing secondary thermostatic waterbath leaching on primary leaching cake meal obtained in the step (3); (5) performing solvent desolventizing on the secondary leaching cake meal obtained in the step (4) so as to obtain degreased selenium-enriched peanut protein powder; (6) performing further protein extraction on the degreased selenium-enriched peanut protein powder obtained in the step (5) so as to obtain a solution; (7) performing vacuum sucking filtration on the solution obtained in the step (6), and collecting a filtrate; and (8) drying precipitation obtained in the step (7), and performing superfine crushing so as to obtain the selenium-enriched peanut isolated protein powder.

The invention discloses a quick construction method of CAR-T (chimeric antigen receptor T-cells) toxicity indictor cells, which comprises the steps of first, constructing a lentiviral plasmid having asequence shown as SEQ NO: 1; second, performing large-scale extraction on the lentiviral plasmid, and performing virus packaging to prepare lentivirus; third, infecting the lentivirus with common cells X; fourth, adding one or more biotin-processed proteins A into universal cells U, and tightly combining with avidin of membranes of the universal cells U to obtain CAR-T toxicity indicator cells. The quick construction method of CAR-T toxicity indictor cells has the advantages that by adding the different biotin-processed proteins A or their combination, the cells U with Luciferase fragments can be converted quickly into various indicator cells suitable for CAR-T toxicity detection, the complex preparation steps are omitted greatly, preparation time is saved, and the cost is reduced.

The invention discloses a method for carrying out enzymatic hydrolysis. The method comprises the following steps: adding water into protein, evenly stirring, transferring the mixture to an electrophoresis tank, carrying out electrophoresis under a voltage of 80 to 120 V, collecting the protein solution after electrophoresis, dissolving the protein by an enzymatic hydrolysis buffer solution containing trifluoroethanol, subjecting the protein to reduction and alkylation treatments, adjusting the pH value of processed protein to 7.5-8.5, then adding trypsase accounting for 90 to 98% of the protein solution, and carrying out enzymatic hydrolysis to obtain polypeptide; wherein the work electrode in the electrophoresis tank is modified by oxidation-reduction enzyme and Zn-Al layered double hydroxides. The provided method can obtain peptides suitable for liquid chromatogram-mass spectrum (LS-MS) analysis, and provides stable and reliable samples for proteomics analysis.

The invention relates to the field of biotechnology and provides a method for producing recombinant proteins from the orb-weaving spider silk in yeast cells. This involves the construction of an expression vector which comprises a DNA sequence encoding a recombinant protein of the orb-weaving spider silk fused with a sequence encoding an ubiquitin-like protein occupying an N-terminal position with respect to the spider silk recombinant protein within the fused protein. The expression of a hybrid gene makes it possible to increase tens of times the production of recombinant spider silk protein, wherein the recombinant protein accumulates in the yeast cells in a water-insoluble fraction in the form of a processed protein free of a hybrid component.The invention also relates to fused proteins comprising sequences of recombinant proteins of the orb-weaving spider silk and of ubiquitin-like proteins, to recombinant DNAs encoding the fused proteins, to host yeast cells and to expression vectors suitable for carrying out the method, and also to producer strains of recombinant proteins of the orb-weaving spider silk.

The invention discloses a method accelerating protein out-phase nucleation. Hydrofluoric acid solution whose volume ratio is 70%, 60%, 50% and 40% is used to treat cover glass surface to obtain four kinds of porous surface substrate material with different features in order; protein solution performs nucleation and grows on the cover glass of the porous surface treated with the above step; the processed protein solution is put in a temperature controller with temperature of 20 DEG C for 2 days; the protein solution is taken out and observed under a microscope to calculate numbers of growing conditions of protein crystal on a porous surface substrate. Due to the adoption of porous surface as nucleation substrate, the success ratio of the protein crystal screening is improved to 46-76% from 2% of background technology.