The application relates to a
protein sample processing method, comprising the following steps: mixing a
protein sample with an alkylating agent to seal free
cysteine, obtaining a sealed sample; centrifuging the sealed sample through an
ultrafiltration membrane, obtaining a membrane sample; the pore size of the
ultrafiltration membrane ranges from 10 kDa to 30 kDa; performing first reduction on the membrane sample, obtaining a first reduced sample; adding a
biotin labeling agent to the first reduced sample, obtaining a labeled sample; enzymatically digesting the labeled sample, obtaining an enzymatically digested sample; adding
avidin microbead
agarose to the enzymatically digested sample to adsorb
biotin-labeled proteins, obtaining an enriched sample; performing reduction and alkylization on the enriched sample, obtaining a processed sample.