Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method

A technology for sunflower oil and protein, applied in the fields of biochemical equipment and methods, enzymes, fat oil/fat production, etc., to achieve the effects of simple equipment, high quality and less pollution

Inactive Publication Date: 2007-04-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no other reports on the direct recovery of sunflower protein from the hydrolyzate

Method used

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  • Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method
  • Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method
  • Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. Add the 0.05mol / L citric acid buffer solution of 750mlpH4.8 to the sunflower seed 150g after pulverizing, stir the complex cellulase that adds 2.5% (w / w shelled sunflower seed dry basis) after being made into slurry, at 50 Enzyme hydrolysis at ℃ for 7h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the free oil yield, which can reach 87%.

[0033] Take 200ml of the filtered hydrolyzate, concentrate it in vacuum until the solid content is about 13%, add 600ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum Th...

Embodiment 2

[0035] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. The sunflower seed 150g after pulverizing is added the 0.05mol / L citrate buffer solution of 750mlph4.8, stirs the compound cellulase that adds 2% (w / w shelled sunflower seed dry basis) after being stirred into slurry, in Enzymatic hydrolysis at 50°C for 5h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the yield of free oil, which can reach 80%.

[0036] Take 200ml of the filtered hydrolyzate, concentrate in vacuum until the solid content is about 9%, add 200ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum ...

Embodiment 3

[0038] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. The sunflower seed 150g after pulverizing is added the 0.05mol / L citrate buffer solution of 900mlpH4.8, after being stirred into slurry, add the complex cellulase of 1% (w / w shelling sunflower seed dry basis), in Enzymatic hydrolysis at 50°C for 5h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the free oil yield, which can reach 78%.

[0039] Take 200ml of the filtered hydrolyzate, concentrate in vacuum until the solid content is about 15%, add 370ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum The prote...

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Abstract

The method relates to the method to extract the sunflower oil and recover the protein by water-enzyme method. The producing procedure comprises the following steps: dry-pulverizing the uncoated sunflower seed, adding citrate buffer to the powder and decomposing the powder by compound cellulose; centrifugalizing to get free oil, emulsion, digest and tablet; high speed centrifugalizing to get some free oil; filtering, vacuum concentrating, alcohol precipitating, centrifugalizing and freeze-drying to recycle the protein. The special treatment in the invention has substantially increased the free oil yield and the quality of the free oil is better than the refined oil. The water-extracting and alcohol-precipitating method to get the protein is simple and efficient; besides, the method has maintained the sunflower protein's nutritive value and functional value very well. Compared with the traditional squeezing and water-extracting technologies, the invention has many advantages such as simple producing equipments, safe operation, less energy expenditure and less pollution.

Description

technical field [0001] The invention discloses a method for extracting sunflower oil and recovering protein by an aqueous enzymatic method, which belongs to the technical field of oil extraction technology by an aqueous enzymatic method. Background technique [0002] Sunflower kernels contain more than 50% oil, which is rich in essential fatty acids, the content of linoleic acid is as high as 66%, and the content of oleic acid is about 15%. The high content of linoleic acid and oleic acid makes sunflower oil more capable of dissolving cholesterol than other vegetable oils. Therefore, long-term consumption of sunflower oil can reduce the cholesterol content in the human body, which is beneficial to the prevention and treatment of cardiovascular diseases and hyperlipidemia , to enhance human health and prolong life, so sunflower oil has become the crown of edible oil with its unique nutritional and health effects. The refined sunflower oil is clear light yellow or bluish yell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C12S3/14C12S3/18C12N9/42
Inventor 许时婴王璋杨瑞金冷玉娴袁搏
Owner JIANGNAN UNIV
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