Process for extracting sunflower seed oil and recovering protein by aqueous enzymatic method
A technology for sunflower oil and protein, applied in the fields of biochemical equipment and methods, enzymes, fat oil/fat production, etc., to achieve the effects of simple equipment, high quality and less pollution
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Embodiment 1
[0032] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. Add the 0.05mol / L citric acid buffer solution of 750mlpH4.8 to the sunflower seed 150g after pulverizing, stir the complex cellulase that adds 2.5% (w / w shelled sunflower seed dry basis) after being made into slurry, at 50 Enzyme hydrolysis at ℃ for 7h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the free oil yield, which can reach 87%.
[0033] Take 200ml of the filtered hydrolyzate, concentrate it in vacuum until the solid content is about 13%, add 600ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum Th...
Embodiment 2
[0035] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. The sunflower seed 150g after pulverizing is added the 0.05mol / L citrate buffer solution of 750mlph4.8, stirs the compound cellulase that adds 2% (w / w shelled sunflower seed dry basis) after being stirred into slurry, in Enzymatic hydrolysis at 50°C for 5h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the yield of free oil, which can reach 80%.
[0036] Take 200ml of the filtered hydrolyzate, concentrate in vacuum until the solid content is about 9%, add 200ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum ...
Embodiment 3
[0038] Shelled sunflower seeds are dry crushed with a traditional Chinese medicine grinder. The sunflower seed 150g after pulverizing is added the 0.05mol / L citrate buffer solution of 900mlpH4.8, after being stirred into slurry, add the complex cellulase of 1% (w / w shelling sunflower seed dry basis), in Enzymatic hydrolysis at 50°C for 5h. Centrifuge at 3000rpm to obtain free oil, emulsion layer, hydrolyzate and precipitate after centrifugation. Carefully take out the emulsion layer and carry out secondary centrifugation with a centrifugal force of 8000g to separate the oil in the emulsion layer. Calculate the free oil yield, which can reach 78%.
[0039] Take 200ml of the filtered hydrolyzate, concentrate in vacuum until the solid content is about 15%, add 370ml of absolute ethanol at about -20°C to precipitate the protein, let it stand for a certain period of time and then centrifuge at 3000rpm, redissolve the precipitate with deionized water, and spin in vacuum The prote...
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