High throughput screening for novel bioactivities

a bioactivity and high throughput technology, applied in the field of identification of new bioactivities, can solve the problems of difficult chemical duplicate, low enantioselectivity, and hampered synthesis of polymers,
US20050040550A1Inactive Publication Date: 2005-02-24DIVERSA CORP
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
DIVERSA CORP
Publication Date
2005-02-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nuclei acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
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Description

[0001] This application is a continuation of U.S. patent application Ser. No. 09 / 848,095, filed May 3, 2001, which is a divisional of U.S. patent application Ser. No. 09 / 636,778, filed Aug. 11, 2000, which is a continuation of U.S. patent application Ser. No. 09 / 098,206, filed Jun. 16, 1998, continuation-in-part of U.S. application Ser. No. 08 / 876,276, filed Jun. 16, 1997, now abandoned. Each of the aforementioned applications are explicitly incorporated herein by reference in their entirety.FIELD OF THE INVENTION

[0002] The present invention relates generally to the identification of new bioactive molecules and particularly to methods for recovering such molecules by co-encapsulation and fluorescence activated cell sorting (FACS). BACKGROUND OF THE INVENTION

[0003] There is a critical need in the chemical industry for efficient catalysts for the practical synthesis of optically pure materials; enzymes can provide the optimal solution. All classes of molecules and compounds that are...

Claims

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