Immunisation against chlamydia trachomatis

Inactive Publication Date: 2005-05-19
CHIRON CORP
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Benefits of technology

[0114] The nucleic acid molecules of the subject invention may be included into an expression cassette for expression of the protein(s) of interest. Usually, there will be only one expression cassette, although two or more are feasible. The recombinant expression cassette will contain in addition to the heterologous protein encoding sequence the following elements, a promoter region, plant 5′ untranslated sequences, initiation codon depending upon whether or not the structural gene comes equipped with one, and a transcription and translation termination sequence. Unique restriction enzyme sites at the 5′ and 3′ ends of the cassette allow for easy insertion into a pre-existing vector.
[0115] A heterologous coding sequence may be for any protein relating to the present invention. The sequence encoding the protein of interest will encode a signal peptide which allows proces

Problems solved by technology

Failure to clear the infection results in persistent immune stimulation and, rather than helping the host, this results in chronic infection with severe consequences

Method used

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  • Immunisation against chlamydia trachomatis
  • Immunisation against chlamydia trachomatis
  • Immunisation against chlamydia trachomatis

Examples

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example 1

[0236] CT242 (SEQ ID 57 and SEQ ID 58) was expressed in E.coli. The recombinant product was purified both as a GST-fusion protein (FIG. 1A; lanes 4 and 5, chromatography fractions 1 and 2, expected molecular weight 42.4 kDa) and as a His-tagged fusion protein (FIG. 1B; lanes 2-4, chromatography fractions 1, 2 and 3, expected molecular weight 16.4 kDa).

[0237] The recombinant protein was used to immunise mice, whose sera were used in a Western blot (FIG. 1C: His-tagged: lanes 12 and 13; GST-fusion: lanes 20 and 21). Lane 12 shows membrane strips stained with pre-immune sera for His-tagged CT242 whilst lane 13 shows membrane strips stained with immune sera for His-tagged CT242. Lane 20 shows membrane strips stained with preimmune sera for GST-fusion CT242 whilst lane 21 shows membrane strips stained with immune sera for GST-fusion CT242.

[0238] These experiments show that CT242 is a surface-exposed and immunoaccessible protein, and that it is a useful immunogen. These properties are n...

example 2

[0239] CT045 (SEQ ID 71 and SEQ ID 72) was expressed in E.coli. The recombinant product was purified as a His-tagged fusion protein (FIG. 2A; lanes 4-6, chromatography fractions 1, 2 and 3, expected molecular weight 55.8 kDa). The recombinant protein was used to immunise mice, whose sera were used in a Western blot (FIG. 2B, lanes 8 and 9) and for FACS analysis (FIG. 2C, K-S value 16.81).

[0240] These experiments show that CT045 is a surface-exposed and immunoaccessible protein, and that it is a useful immunogen. These properties are not evident from the sequence alone.

example 3

[0241] CT381 (SEQ ID 105 and SEQ ID 106) was expressed in E.coli. The recombinant product was purified both as a GST-fusion protein (FIG. 3A; lanes 2 and 3, chromatography fractions 1 and 2, expected molecular weight 52.7 kDa) and as a His-tagged fusion protein (FIG. 3A; lanes 7-9, chromatography fractions 1, 2 and 3, expected molecular weight 26.7 kDa). The recombinant protein was used to immunise mice, whose sera were used in a Western blot (FIG. 3B: His-tagged: lanes 6 and 7; GST-fusion: lanes 16 and 17) and for FACS analysis (FIG. 3C: GST-tagged, K-S value 35.98; FIG. 3D: His-tagged, K-S value 32.54).

[0242] These experiments show that CT381 is a surface-exposed and immunoaccessible protein, and that it is a useful immunogen. These properties are not evident from the sequence alone.

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Abstract

The published genomic sequence of Chlamydia trachomatis reveals over 1000 putative encoded proteins but does not itself indicate which of these might be useful antigens for immunisation and vaccination or for diagnosis. This difficulty is addressed by the invention, which provides a number of C.trachomatis protein sequences suitable for vaccine production and development and/or for diagnosis purposes.

Description

[0001] All documents cited herein are incorporated by reference in their entirety. TECHNICAL FIELD [0002] This invention is in the field of immunisation against chlamydial infection, in particular against infection by Chlamydia trachomatis. BACKGROUND ART [0003] Chlamydiae are obligate intracellular parasites of eukaryotic cells which are responsible for endemic sexually transmitted infections and various other disease syndromes. They occupy an exclusive eubacterial phylogenic branch, having no close relationship to any other known organisms—they are classified in their own order (Chlamydiales) which contains a single family (Chlamydiaceae) which in turn contains a single genus (Chlamydia, also referred to as Chlamydophila). A particular characteristic of the Chlamydiae is their unique life cycle, in which the bacterium alternates between two morphologically distinct forms: an extracellular infective form (elementary bodies, EB) and an intracellular non-infective form (reticulate bo...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K39/118A61K48/00A61P31/04C07K14/295C12N15/09G01N33/53G01N33/569
CPCA61K39/118A61P15/00A61P31/04A61P31/10A61K39/00
Inventor GRANDI, GUIDORATTI, GIULIO
Owner CHIRON CORP
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