Fermented plant extracts, methods of production and uses

a technology of plant extracts and fermented extracts, applied in the field of plant extracts, can solve the problems of affecting the lipooxygenase, and achieve the effects of increasing the amount of active agents and increasing the bioavailability of the components of kefirated tisan

Active Publication Date: 2011-06-02
KEFIPLANT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The patent describes a method for creating a food supplement using a fermented plant extract called kefir. Kefir is made from a combination of yeast and bacteria, which work together to create a stable and effective food supplement. The fermentation process allows the plant extract to release active compounds that can be absorbed by the intestinal lining, improving their biological activity. The patent also describes the use of kefir in a variety of applications, such as improving skin health, treating allergies, and fighting inflammation. The kefir grain used in the fermentation process is a natural biological entity that is safe and effective without needing any additional activators. The patent also discusses the process of kefir grain fermentation and the importance of balancing the substrate and seeding strains for continuous fermentation. Overall, the patent provides a technical solution for creating a food supplement with improved absorption and biological activity using kefir fermentation."

Problems solved by technology

However, at low concentrations, only lipooxygenase is affected.

Method used

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  • Fermented plant extracts, methods of production and uses
  • Fermented plant extracts, methods of production and uses
  • Fermented plant extracts, methods of production and uses

Examples

Experimental program
Comparison scheme
Effect test

example i

Analysis of the Aromatic Profiles

[0094]For the purpose of analyzing the aromatic profiles of the fermented extracts, three types of extracts may be prepared for the purpose of analyzing them by chromatography in gaseous phase combined with mass spectrometry (CPG-SM):

[0095]1. Extracts obtained by hydrodistillation of plants: vegetable starting material, 500 mL water, duration of hydrodistillation: 8 hours. The quantity of vegetable starting material used varies depending on the nature of the plant. Example: thyme: 7.86 g of vegetable matter, essential oil obtained: 0.19 g. Extraction yield: 1.07 g.

[0096]2. SDE extract (Simultaneous Extraction / Distillation) of the type Lickens & Nickerson of plant infusions: extraction protocol: 500 mL of infusion, 30 mL of pentane (flask)+15 mL in the loop, 2 hours of extraction, recovery of the extraction solvent and mild evaporation under nitrogen flow up to 2 mL—keeping samples at −20° C. until analysis.

[0097]3. SPME extracts (Solid Phase Microex...

example ii

CPG-SM Analyses

[0098]In order to analyze the fermented extracts, CPG-SM analyses may also be carried out. The chromatographic conditions must first be optimized for each type of plant infusion and each type of extract. However, in a general manner, the analyses may be carried out on an Agilent column J&W, DB5-MS (5% phenylmethylsiloxane), 30 m long×250 μm internal diameter×1 μm film thickness. The analyses are carried out under a constant flow of helium: 1.4 mL / min (average speed of 30 cm / sec). The temperatures of the injector and of the detector (transfer line) are kept at 290° C. for the analyses of liquid extracts. For the SPME extracts, the temperature of the injector is 250° C. (desorption temperature of the volatile compounds of the PDMS fiber). The mode of injection depends on the type of extract that is analyzed. For the Lickens & Nickerson and hydrodistillation extracts, the injections were carried out in split mode and in splitless mode for the SPME extracts. Programming o...

example iii

Ethanol, P-Cymenem Thymol and Carvacol Analysis

[0099]For some extracts, it is also possible to titrate ethanol, p-cymene, thymol and carvacrol. The possibility of directly titrating p-cymene, thymol and carvacrol in the matrix was explored in the case of thyme and oregano infusions. A FFAP column 25 m long×0.32 mm internal diameter×0.3 μm film thickness was used. In particular, thyme and oregano infusions were analyzed by CPG / FID. 1 μL of each infusion was injected in an injector at 250° C. Temperature programming is the following: 40° C. to 220° C. (5 min) (5° C. / min). The standards of p-cymene and thymol were injected as external standards to obtain calibration curves and as internal standard for identification. Titration of ethanol was also carried out by chromatography in gaseous phase combined with flame ionization (CPG / FID) for some infusions that were fermented from a calibration curve by directly injecting the solutions.

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Abstract

The present application concerns plant extracts which have been fermented with kephir grains, methods of production of these extracts, a powder comprising these extracts and compositions comprising these extracts. Since these extracts have a high content of aglycone active principles, their biological activities are high and their applications are varied.

Description

TECHNICAL FIELD OF THE INVENTION[0001]The present invention relates to plant extracts which have been fermented with kefir grains, to methods of production of these extracts, as well as to the use of these fermented extracts in animal and human health.DESCRIPTION OF PRIOR ART[0002]Aromatic and medicinal plants have been and are presently important in the preventive and curative therapy of human and animal health. About 80% of the active substances used in occidental medicine come from plants.[0003]From the traditional to the modern medicine, the evolution in the search for active principles originating from plants was carried out by phytochemical screening. First, the choice dealt with the whole plant, then certain parts of the plants (flowers, leaves, roots, barks . . . ) and subsequently with plant extracts (aqueous, alcoholic . . . ) and mixtures of active principles, and finally purified active principles. Today, these derivatives are usually obtained by hemisynthesis.[0004]In a...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61K35/20A61K36/00A61K36/889A61K36/82A61K36/28A61K36/53A23K1/18A01P15/00A61P1/16A61P25/06A61P1/00A61P31/00A23L27/00A61K36/06
CPCA61K36/82A61K36/53C12P1/04C12P7/22C12P7/40C12P1/02A61K36/28A23C9/20A23F3/166A23K1/007A23K1/1813A23K1/184A23K1/1893A23L1/3002A61K9/1688A61K35/20A61K36/06A61K2300/00A23K10/12A23K50/10A23K50/30A23K50/60A23L33/105A61P1/00A61P1/16A61P11/14A61P25/06A61P25/22A61P31/00A61P39/00
InventorROLLAN, SERGEHOULE, CHANTALEDESHAYES, CHRISTIAN
OwnerKEFIPLANT