Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Information acquisition method

a technology of information acquisition and information acquisition, applied in the field of information acquisition methods, can solve the problems of low impaired distribution state of a substance, and difficult to achieve both soft ionization and high ionization efficiency of a substance to be analyzed, so as to achieve high mass spectrometry efficiency and improve ionization efficiency

Inactive Publication Date: 2012-07-19
CANON KK
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016]The inventors of the present invention have found that both of soft ionization and high ionization efficiency of a substance to be analyzed can be achieved by the following method, and thus, the present invention has been completed.
[0018]The present invention can simultaneously achieve an improvement in ionization efficiency by protonation of a substance to be analyzed due to attachment of an organic acid, and fragmentation inhibition by attachment of a polyhydric alcohol. The synergistic effect thereof allows highly efficient mass spectrometry and imaging in a wide molecular weight range.

Problems solved by technology

However, even in the above-mentioned method, it is still difficult to achieve both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed.
873 allows effective soft ionization of a substance to be analyzed, but it has a problem in that a distribution state of a substance is impaired in a sample preparation step.
929 each have a problem in that ionization efficiency of a substance to be analyzed is low due to their generally small cationizing abilities on the substance to be analyzed as compared to a proton-donating action with an acid.
However, its main aim is not effective soft ionization, and hence there is a problem in that, when a substance to be analyzed is a high molecular weight substance, fragmentation of an ion occurs, which makes it difficult to assign the substance to be analyzed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0045]In this example, mass spectrometry was carried out using a TOF-SIMS apparatus (TOF-SIMS 5 type manufactured by ION-TOF). A glass substrate with an ITO vapor deposition layer (Sigma-Aldrich, 1×1 inch, #576352) was used as a support for a specimen. A frozen tissue section of murine pancreas (thickness: 5 μm) was mounted onto the substrate and adhered thereto by thawing. After that, the whole was shaken for 5 minutes while distilled water exchange being carried out, followed by a drying treatment in a vacuum desiccator for 30 minutes. To the entire surface of the dried tissue section on the substrate, a 10% (w / w) glycerol (MP: 17.8° C., BP: 290° C.)−0.1% (w / w) perfluorosuberic acid (BP: 205° C.) aqueous solution as an ionization assisting reagent was applied using a micropipetter to such an extent that the specimen became wet. The sample was placed in the TOF-SIMS apparatus and analyzed under the following measurement conditions. The detection position and detection sensitivity o...

example 2

[0057]The same operation as that in Example 1 was carried out except that a 10% (w / w) ethylene glycol−0.1% (w / w) perfluorosuberic acid aqueous solution was used as the ionization assisting reagent. The detection sensitivity of a peptide component at m / z 1,197 was evaluated in order to estimate an effect of the ionization assisting reagent. Table 1 shows the results.

example 3

[0058]The same operation as that in Example 1 was carried out except that a 10% (w / w) diethylene glycol−0.1% (w / w) perfluorosuberic acid aqueous solution was used as the ionization assisting reagent. The detection sensitivity of a peptide component at m / z 1,197 was evaluated in order to estimate an effect of the ionization assisting reagent. Table 1 shows the results.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF2)COOH and having a boiling point of 150° C. or more, and a polyhydric alcohol having a melting point of 20° C. or less and a boiling point of 150° C. or more at normal pressure, the organic acid, which is a component of the ionization assisting reagent, effectively donates a proton to the substance to be analyzed, thereby improving ionization efficiency, and the polyhydric alcohol, which is a component of the ionization assisting reagent, inhibits fragmentation.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to an information acquisition method for acquiring information including a two-dimensional distribution state of a substance to be analyzed by an imaging mass spectrometry method using time-of-flight secondary ion mass spectrometry (hereinafter, referred to as TOF-SIMS), and more particularly, to an information acquisition method including subjecting an organic substance such as a lipid or a protein as a substance to be analyzed to high-sensitivity analysis.[0003]2. Description of the Related Art[0004]In the fields of biochemistry and medicine, there is a demand for acquisition of distribution information of a specific substance that constructs a biological tissue. As an example, in the field of medicine such as pathology, when distribution information of a specific antigen protein at a “cellular level” can be acquired in definitive diagnosis, a type of the disease can be determined and a t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): H01J49/26H01J49/40
CPCH01J49/142Y10T436/24Y10T436/25875Y10T436/25
Inventor SATOH, SHUYAHASHIMOTO, HIROYUKI
Owner CANON KK
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com