Information acquisition method

a technology of information acquisition and information acquisition, applied in the field of information acquisition methods, can solve the problems of low impaired distribution state of a substance, and difficult to achieve both soft ionization and high ionization efficiency of a substance to be analyzed, so as to achieve high mass spectrometry efficiency and improve ionization efficiency

Inactive Publication Date: 2012-07-19
CANON KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018]The present invention can simultaneously achieve an improvement in ionization efficiency by protonation of a substance to be analyzed due to attachment of an organic acid, and

Problems solved by technology

However, even in the above-mentioned method, it is still difficult to achieve both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed.
873 allows effective soft ionization of a substance to be analyzed, but it has a problem in that a distribution state of a substance is impaired in a sample preparation step.
929 each have a pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0045]In this example, mass spectrometry was carried out using a TOF-SIMS apparatus (TOF-SIMS 5 type manufactured by ION-TOF). A glass substrate with an ITO vapor deposition layer (Sigma-Aldrich, 1×1 inch, #576352) was used as a support for a specimen. A frozen tissue section of murine pancreas (thickness: 5 μm) was mounted onto the substrate and adhered thereto by thawing. After that, the whole was shaken for 5 minutes while distilled water exchange being carried out, followed by a drying treatment in a vacuum desiccator for 30 minutes. To the entire surface of the dried tissue section on the substrate, a 10% (w / w) glycerol (MP: 17.8° C., BP: 290° C.)−0.1% (w / w) perfluorosuberic acid (BP: 205° C.) aqueous solution as an ionization assisting reagent was applied using a micropipetter to such an extent that the specimen became wet. The sample was placed in the TOF-SIMS apparatus and analyzed under the following measurement conditions. The detection position and detection sensitivity o...

example 2

[0057]The same operation as that in Example 1 was carried out except that a 10% (w / w) ethylene glycol−0.1% (w / w) perfluorosuberic acid aqueous solution was used as the ionization assisting reagent. The detection sensitivity of a peptide component at m / z 1,197 was evaluated in order to estimate an effect of the ionization assisting reagent. Table 1 shows the results.

example 3

[0058]The same operation as that in Example 1 was carried out except that a 10% (w / w) diethylene glycol−0.1% (w / w) perfluorosuberic acid aqueous solution was used as the ionization assisting reagent. The detection sensitivity of a peptide component at m / z 1,197 was evaluated in order to estimate an effect of the ionization assisting reagent. Table 1 shows the results.

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PUM

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Abstract

Provided is a method that achieves both of soft ionization and high ionization efficiency of a substance to be analyzed at each measurement site without impairing a two-dimensional distribution state of the substance to be analyzed in desorption ionization mass spectrometry of a substance to be measured. By applying, to a substance to be analyzed, an ionization assisting reagent including an organic acid including a functional group represented by —(CF2)COOH and having a boiling point of 150° C. or more, and a polyhydric alcohol having a melting point of 20° C. or less and a boiling point of 150° C. or more at normal pressure, the organic acid, which is a component of the ionization assisting reagent, effectively donates a proton to the substance to be analyzed, thereby improving ionization efficiency, and the polyhydric alcohol, which is a component of the ionization assisting reagent, inhibits fragmentation.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to an information acquisition method for acquiring information including a two-dimensional distribution state of a substance to be analyzed by an imaging mass spectrometry method using time-of-flight secondary ion mass spectrometry (hereinafter, referred to as TOF-SIMS), and more particularly, to an information acquisition method including subjecting an organic substance such as a lipid or a protein as a substance to be analyzed to high-sensitivity analysis.[0003]2. Description of the Related Art[0004]In the fields of biochemistry and medicine, there is a demand for acquisition of distribution information of a specific substance that constructs a biological tissue. As an example, in the field of medicine such as pathology, when distribution information of a specific antigen protein at a “cellular level” can be acquired in definitive diagnosis, a type of the disease can be determined and a t...

Claims

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Application Information

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IPC IPC(8): H01J49/26H01J49/40
CPCH01J49/142Y10T436/24Y10T436/25875Y10T436/25
Inventor SATOH, SHUYAHASHIMOTO, HIROYUKI
Owner CANON KK
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