Composition and method for improving stability and extending shelf life of probiotic bacteria and food products thereof
a technology of probiotic bacteria and food products, applied in the field of probiotics and food products, can solve the problems of lack of extended shelf life, and achieve the effect of increasing resistance and prolonging shelf li
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Preparation and Testing of an Exemplary Formulation
[0126]Preparation of an exemplary, illustrative formulation, described herein as Formula I, was performed as described below. Tests performed on Formula I are also described below.
[0127]For the preparation of Formula I, trehalose dihydrate 160 g, Maltodextrin DE15 314 g, L-Cystein-HCl Monohydrate 6 g and the bacteria BB12 (Bifidobacteria-BB12) 120 g were loaded into Innojet ventilus machine to receive a dry blend. Lauric acid 270 g was melted at 50° C. using a heating plate while stirring. Then hot melt of lauric acid was sprayed onto the above dry blend under an inert atmosphere using nitrogen. The temperatures of pump head, liquid, and spray pressure were set at 60° C. Core particles were therefore formed, based on a hot melt granulation process.
[0128]Next, the various layers were coated over the core particles as follows. For the first (innermost) layer, lauric acid 315 g was melted at 50° C. using a heating plate while stirring....
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