Olive derived cell culture and methods for preparing and using the same

Abstract

The present application describes a large process for the in vitro production of an olive cell culture.The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro and a method of treating metabolic syndrome disorders, such as, high cholesterol level, comprising administering an effective amount of the composition. The cell line callus culture of olive cells manufactured according to the process of the invention includes high level of hydroxytyrosoll, tyrosol, oleoropein and verbascoside.

Description

FIELD OF THE INVENTION[0001]The invention is directed to olive derived cell cultures, a process for the large scale production of such cell cultures, as well as methods of using the same.BACKGROUND OF THE INVENTION[0002]Large scale processes are known in the art and are necessary for the industrial production of various materials. Since large scale processes cannot be performed by the same means as small scale processes, specific processes for the large scale production of materials must be designed, even if small scale processes exist.[0003]Nutraceuticals are sometimes prepared using synthetic processes that provide the desired active ingredients, e.g., polyphenols, which are naturally found in fruit cells. However, the use of synthetic processes does not provide the natural ingredients along with the active ingredients, which sometimes contribute to the efficiency of the formulation.[0004]Other types of nutraceuticals are prepared from the natural plants; however, all known large ...

AI Technical Summary

Benefits of technology

The invention provides a method for reducing the risk factors for cardiovascular disease, treating atherosclerotic plaque, protecting LDL particles from oxidative damage, maintaining normal blood HDL-cholesterol concentrations, decreasing high blood pressure, and maintaining normal haemostatic function. The method includes administering an effective amount of a composition made from olive cells grown in vitro. The olive cells can be derived from various parts of the olive tree such as the cross section, pulp, seed, petiole, or leaf.

Problems solved by technology

Since large scale processes cannot be performed by the same means as small scale processes, specific processes for the large scale production of materials must be designed, even if small scale processes exist.

However, the use of synthetic processes does not provide the natural ingredients along with the active ingredients, which sometimes contribute to the efficiency of the formulation.

However, when plants containing polyphenols, for example, are extracted, the final product may be bitter.

Also, only certain parts of the plant may be successfully extracted since only they contain the desired amounts of the active ingredients.

Small scale processes for the preparation of fruit cells are known in the art; however, large scale processes are more difficult to design since they tend to amplify the production of the primary metabolites, while minimizing the productions of the secondary metabolites.

Since active ingredients, such as polyphenols, are secondary metabolites their production in large-scale processes is complex.

However, it has been shown that the therapeutic effect of fruit extracts is dependent on species, location, year (annual climate), processing etc. and therefore reliance on natural fruits as a source of these regulatory compounds does not lead to a homogeneous or consistent supply of material.

Furthermore, fruits are often contaminated by residual fungicides, pathogens, pesticides and pollutants.

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