The invention relates to a method for extracting
RNA (Ribonucleic Acid) of eremophyte Reaumuria songarica Maxim. The method comprises the following steps of: scattering PVPP (Crosslinked
Polyvinyl Pyrrolidone) to leaves of Reaumuria songarica Maxim,
grinding, centrifuging an obtained solution, taking supernate, adding
chloroform and isoamylol which are equal in volume, and centrifuging; taking supernate again, adding
phenol,
chloroform and isoamylol which are equal in volume, and centrifuging; taking supernate, adding
chloroform and isoamylol which are equal in volume, and centrifuging again; taking supernate, adding precooled absolute ethyl
alcohol and LiC1, mixing uniformly, and performing ice bath; centrifuging again, pouring supernate, adding precooled absolute
alcohol and
sodium acetate, uniformly mixing, placing in a refrigerator, and centrifuging; and abandoning supernate, rinsing twice with 75% ethyl
alcohol, centrifuging, collecting precipitates, airing to obtain a pure
RNA sample, and dissolving the
RNA sample in DEPC (Diethylpyrocarbonate) water. According to the invention, by means of effectively removing
polyphenol,
polysaccharide,
protein,
ion substances and other
secondary metabolite, the pure RNA sample is obtained; and in addition, the yield is high and less time is consumed. Compared with the prior art, the method disclosed by the invention is simple to operate, and RNA is not degraded by RNase (
Ribonuclease) and is good in integrity.