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Aspergillus niger lipase and its preparation method

A technology of Aspergillus niger and lipase, applied in the field of lipase and its preparation, can solve the problems of narrow pH value range and instability of Aspergillus niger lipase, and achieve the effects of wide applicable pH value range, wide application and simple process

Active Publication Date: 2008-02-13
ANHUI LEVEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Aspergillus niger lipase obtained by these two methods has a narrow optimum pH range and is unstable under alkaline conditions

Method used

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  • Aspergillus niger lipase and its preparation method
  • Aspergillus niger lipase and its preparation method
  • Aspergillus niger lipase and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Strain: Aspergillus niger strain S-7749

[0023] Seed preparation of strains: the strain S-7749 was grown at 27°C by expanding the potato slant seeds into eggplant bottles for cultivation, and then expanding into K-flasks for cultivation, and the cultivation time for each step was 100 hours.

[0024] Fermentation:

[0025] 1) Primary seed tank fermentation

[0026] Medium (%):

[0027] Soya bean cake flour 2.6

[0028] Cornstarch 0.5

[0029] NaNo 3 0.5

[0030] Sodium citrate 0.08

[0031] MGSO 4 0.04

[0032] Inoculation method: spore inoculation

[0033] Training conditions:

[0034] Temperature: 26°C

[0035] Ventilation volume: 1:1V / V

[0036] pH: 6.3-6.4

[0037] Culture period: 17 hours

[0038] 2) Secondary seed tank fermentation

[0039] Medium (%): fermented in the same level of seed tank

[0040] Inoculation method: pressure difference transplantation method

[0041] Culture conditions

[0042] Temperature: 26°C

[0043] Venti...

example 2

[0067] Similar to Example 1, the difference is that the temperature in the seed preparation process of the bacteria is 26°C, and the cultivation time in the eggplant bottle and the K bottle is 120 hours; the fermentation temperature in the primary seed tank is 27°C, and the cultivation period is 18 hours; the fermentation temperature in the secondary seed tank was 27°C, and the culture cycle was 10 hours; the fermentation temperature in the fermenter was 25°C, and the fermentation cycle was 44 hours; After 18 hours of fermentation, it increased to 1:1.4 V / V, and after 35 hours of fermentation, it increased to 1:1.6 V / V.

[0068] The enzyme activity of the liquid after the fermentation in the fermenter was measured was 3216U / ml.

example 3

[0070] Similar to Example 1, the difference is that the temperature in the seed preparation process of the bacteria is 25°C, and the cultivation time in the eggplant bottle and the K bottle is 80 hours; the fermentation temperature in the primary seed tank is 25°C, and the cultivation period is 16 hours; the fermentation temperature in the secondary seed tank was 25°C, and the culture cycle was 8 hours; the fermentation temperature in the fermenter was 25°C, and the fermentation cycle was 42 hours; After 15 hours of fermentation, it increased to 1:1.4 V / V, and after 30 hours of fermentation, it increased to 1:1.6 V / V. .

[0071] The enzyme activity of the liquid after the fermentation in the fermenter was measured was 3016U / ml.

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Abstract

The invention relates to an Aspergillus niger lipase and its preparing process, which consists of separating and screening wild strains S-8341 of Aspergillus niger in nature, obtaining fine production strains S-7749 through industrial microorganism mutagenesis breeding, subjecting the strains to enlargement culture and low temperature high oxygen fermentation to obtain aspergillus niger lipase whose N-terminal amino acid sequence is ATADAAAFPDLHRAAKLSSA.

Description

field of invention [0001] The present invention relates to lipase and its preparation method, especially microbial lipase and its preparation method. Background technique [0002] In recent years, the research on microbial lipase has increased significantly, because microbial lipase has been widely used in food industry, flour industry, washing industry, pharmaceutical industry, textile industry, feed industry, biochemical industry and environmental protection industry. The space is quite wide. At present, as many as 65 genera of microbial lipase-producing bacteria have been reported at home and abroad, including: bacteria, 28 genera; filamentous fungi, 23 genera; yeast, 10 genera; actinomycetes, 4 genera. Filamentous fungi secrete enzymes extracellularly and are excellent lipase-producing strains. At present, the strains with the strongest enzyme-producing ability are mainly concentrated in Rhizopas, Aspergillus, Penicillium, Mucor, Phycomyces and other genera. It has be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/20C12R1/685
Inventor 吴松刚施巧琴王剑英张清辉赵燕玉
Owner ANHUI LEVEKING BIOTECH CO LTD
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