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Preparing method of medical nerve transplant containing silk element

A technology of nerve transplantation and silk, applied in the field of medical nerve grafts, can solve the problems of difficult matching of tissue structure and size, long-term denervation of the transplanted donor area, limited nerve sources, etc., and achieve the effect of good biocompatibility

Active Publication Date: 2008-04-23
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In autologous nerve transplantation, it has not been widely used clinically because of the limited source of nerves for transplantation, difficulty in matching tissue structure and size, and long-term denervation of the donor site.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of silk fibroin fiber (prepared according to conventional methods or commercially available finished silk fibroin fiber) or the following method: the silk in weak alkali solution (0.1-10% sodium carbonate or 0.1-10% potassium carbonate) Heat treatment at 50-100°C, and then wash the treated fibers with distilled water to obtain silk fibroin fibers, a part of which is used in step (2), and a part of which is used as a fiber support in step (3).

[0021] (2) Preparation of silk fibroin catheter: Dissolve the above-mentioned silk fibroin fibers in a three-component solution of calcium chloride, ethanol and water at 25-80°C (for example, 25°C, 50°C, 80°C). In the elementary system (molar ratio is calcium chloride: ethanol: water=1: 2: 8), dissolving time is 0.5~6 hours (example 0.5,3,6 hours) the mixed solution of dissolving is packed into cellulose film bag, Dialyze against distilled water. Then inject the dialyzed silk fibroin solution into the mould, dry ...

Embodiment 2

[0024] A preparation method of a medical nerve graft containing a silk fibroin catheter, the preparation method comprising the following steps:

[0025] (1) Preparation of silk fibroin conduits: the same method as in Example 1 was used to obtain silk fibroin conduits.

[0026] (2) Directly use the silk fibroin catheter for nerve regeneration, or fill it with other substances for nerve regeneration.

[0027] Or embed the prepared silk fibroin catheter into a fibrous scaffold made of other materials (such as polyglycolic acid (PGA), polylactic acid (PLA), chitosan, chitin) for nerve regeneration. The method for making fiber scaffolds from polyglycolic acid (PGA), polylactic acid (PLA) and chitosan is the same as the content of Chinese patent CN1126515C. The method for preparing the fiber support from chitin is: dissolving chitosan in a weak acid solution, the weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), and the concentration is 2 to 15% (example 2%, ...

Embodiment 3

[0029] A preparation method of a medical nerve graft containing a silk fibroin catheter, the preparation method comprising the following steps:

[0030] (1), preparation of silk fibroin-containing catheter: the silk fibroin fiber obtained by the same method as in Example 1 was dissolved in calcium chloride at 25-80°C (eg 25°C, 50°C, 80°C) In the ternary system of , ethanol and water (molar ratio is calcium chloride: ethanol: water=1: 2: 8), dissolving time is 0.5~6 hours (example 0.5, 2, 6 hours) the mixed solution of dissolving into a cellulose film bag and dialyzed with distilled water to obtain a silk fibroin protein solution.

[0031] Dissolve chitosan with weak acid, weak acid is acetic acid (or phosphoric acid, citric acid, lactic acid), concentration is 1~15% (example 1%, 8%, 15%), then chitosan solution is dissolved in silk fibroin The solutions are mixed in a certain weight ratio (for example: 1:2, 1:1, 2:1). Then inject the above-mentioned mixed solution into the m...

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PUM

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Abstract

The medical nerve graft is formed from catheter or catheter and fibre scalfold, and is characterized by that at least one of catheter and fibre scalfold contains silk bambycin protein component. Its preparation method includes the following steps: making natural silk into silk bambycin protein fibre; preparing said silk bambycin protein fibre into catheter containing silk bambycin protein, and combining the catheter and fibre scalfold.

Description

Technical field: [0001] The invention relates to a medical nerve graft for bridging defective nerves. Background technique: [0002] Clinically, peripheral nerve defects caused by trauma (or surgery) are relatively common, but when long-distance nerve defects cannot be compensated for by end-to-end sutures, grafts have to be used to bridge and repair them. Although it has been more than 100 years to find and develop a more suitable graft, except for the autologous nerve which has become the first choice for bridging the nerve defect, it has not yet found a more ideal, recognized and widely used graft in humans. Clinically widely used nerve graft substitutes. As for autologous nerve transplantation, it has not been widely used clinically because of the limited source of nerves for transplantation, difficulty in matching tissue structure and size, and long-term denervation of the transplanted donor site. [0003] With the emergence and development of tissue engineering, it p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/02A61L27/22
CPCA61L31/005A61L31/047A61L31/129A61L27/227A61K38/012A61L27/48A61L2430/32A61L27/3604A61F2/06A61K35/64A61L27/40A61L27/22
Inventor 顾晓松杨宇民丁斐张沛云刘炎刘梅陈雪梅王晓冬
Owner NANTONG UNIVERSITY
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