Highly effective phosphorus removal bacteria and its produced bacteria formulation
A phosphorus-accumulating bacteria and high-efficiency technology, applied in the field of biological high, can solve the problems of excessive phosphorus concentration in effluent, unstable water quality, difficult to restore, etc., achieve stable phosphorus concentration in effluent, improve and protect the ecological environment, and low production and use costs. Effect
Inactive Publication Date: 2008-07-09
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
In order to understand the microbiology and molecular mechanism of EBPR, many researchers at home and abroad have conducted a lot of research on the microbiology and genetics of the EBPR process. Little is known about basic theories such as the PAOs of EBPR and the key genes for the formation of poly-P
Therefore, the behavior of the EBPR system during operation is unpredictable. On the one hand, the biological phosphorus removal system of the newly built sewage treatment plant starts slowly; on the other hand, in the actual large-scale sewage treatment operation, the water quality will be unexpectedly unstable. and sudden deterioration, resulting in a sharp decline in the phosphorus removal effect of the sewage treatment system, and the concentration of phosphorus in the effluent seriously exceeds the standard, and it is difficult to recover
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[0019] 1. Isolation and identification of strains
[0020] Adopting the principle of large throughput and high flow rate, the high-efficiency phosphorus accumulating bacteria GM6 was isolated from activated sludge by means of plate gradient dilution coating, poly-P and PHB staining and blue-white screening. After physiological and biochemical identification, 16S rDNA sequence analysis and homology comparison, GM6 was initially identified as Pseudomonas putida. When GM6 is cultured in synthetic wastewater, the optimum initial pH is 6.5, and the optimum growth temperature is 27°C. When the initial pH value is greater than 8.0 or less than 5.0, or when the temperature is less than 5°C or greater than 37°C, the growth is slow; The amount of liquid had little effect on the growth of GM6. The main biological characteristics are G-, the bacteria are rod-shaped, the size is about 0.7×2.5μm, facultative anaerobic; indole reaction, oxidase and nitrate reduction reaction are positive; m...
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The bacterium agent in this invention is used to biologically strenghen dephosphor- ization process in urban sewage treatment plant. It has mainly two uses: quickly start the biodephosphorization ability of new sewage treatment plant; resume the strong biodephosphorization ability of exasperate dephosphorization system. It is made from high efficient ployphospher bacterial strain GM6, and it is odor Pseudomonas putida from appraising result. Its main biocharacteristic is G-. Its thalli is rhabditiform with the size of 0.7*2.5mu m, and it is facultative anaero-bic; it is positive in indole reaction, oxidase and sulfate reduction reaction; while negative in methyl red test, V-P reaction, citrate test and hydrogen sulfide reaction; the number of the strain 16S rDNA is DQ133506. The strain can be used to sewage dephosphorization.
Description
1. Technical field [0001] The invention relates to a high-efficiency phosphorus-accumulating bacterium and the bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to remove phosphorus in sewage, and are suitable for biological phosphorus removal in urban sewage treatment plants. 2. Technical Background [0002] Eutrophication of water body is one of the top ten environmental problems in the world. The input of nitrogen and phosphorus, especially phosphorus, is the key factor causing water eutrophication. The discharge of urban domestic sewage is one of the main sources of phosphorus pollution in water bodies. In order to solve the harm caused by phosphorus pollution, it is necessary to effectively treat the sewage before it enters the water body and reduce the phosphorus concentration in the discharged sewage. [0003] In the biological phosphorus removal of wastewater, the EBPR process is the mainstream process, and its s...
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IPC IPC(8): C12N1/20C02F3/34C02F1/58C12R1/40
Inventor 李顺鹏蔡天明管莉菠崔中利
Owner NANJING AGRICULTURAL UNIVERSITY