Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same
A technology of microbial preparations and pseudomonas, which is applied in the field of environment-affinity microbial preparations, can solve problems such as crop quality degradation, soil nutrient imbalance, and river eutrophication, and achieves reduction in usage, increase in yield, and promotion of plant growth. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Isolation of soil microorganism RRj228
[0034] The isolation method of soil microorganism RRj228 is as follows.
[0035] like figure 1 A rice seed germinated in sterile medium was transferred to 0.6% agarose medium as indicated. A 0.5 g soil sample (from St. Petersburg, Russia) was inoculated 1-2 cm away from the rice root, and the culture medium was incubated at 28°C for 48 hours. After the roots were crushed to form a suspension, the suspension was applied to an LC medium (the medium contained 10 grams of tryptone for bacterial culture, 5 grams of yeast extract, MgSO 4 ·7H 2 O 8 g, 1 M Tris 1 mL and distilled water 1 L) to isolate strains.
Embodiment 2
[0037] Identification of Soil Microorganism Rj228
[0038] A. Physiological and biochemical characteristics
[0039] The physiological and biochemical properties of the RRj228 strain are shown in Table 1 below.
[0040] The RRj228 strain is rod-shaped with a size of 2.0-3.0×0.5-0.7 microns, such as Figure 2a and 2b It has flagella as shown. When the strain was cultured in PDB medium for 24 hours, yellowish reddish colonies formed. The RRj228 strain is a Gram-negative bacterium that undergoes aerobic metabolism and has catalase and oxidase activities. When the carbon source added to the medium is arabinose, fructose, galactose, glucose, mannitol, sucrose, glycerol or xylose, the strain will produce acid, and when the carbon source added to the medium is lactose, dextrin, ramnose , sorbitol, maltose or galactitol, the strain will produce alkali. Additionally, if image 3 As shown, the RRj228 strain of the present invention is characterized by being present outside the ...
experiment Embodiment 1
[0049] Plant growth promoting effect of Pseudomonas RRj228
[0050] In order to examine the plant growth promoting effect of Pseudomonas RRj228, experiments were carried out on maize seedlings. The root end 3 mm of corn was immersed in the culture suspension of this strain for 1.5 hours, and the roots of other corn were immersed in the sterile culture solution as a control. The corn seedlings were placed on wet filter paper in a plastic box, and then the root end at the starting line was covered with filter paper, and cultured at 28°C for 24 hours. The length of newly elongated roots was measured and repeated 10 times, and the maize growth-promoting effect of the RRj228 strain is shown in Table 2 below.
[0051] [Table 2]
[0052] Growth-promoting effect of RRj228 strain on maize
[0053]
[0054] As shown in Table 2, the stock solution (4.5 × 10 9 c.f.u / ml) was diluted 1:50 and inoculated, the growth promotion level of Pseudomonas RRj228 of the present invention was re...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More