Authenticating gram positive bacteria species and method for testing drug resistant gene and dedicating kit

Abstract

This invention discloses a method of authenticating species of gram positive bacterium and examining drug resistant gene, special reagent kit. This kit contains species special gene of amplified gram positive bacterium and antithetical couplet of primer gene and gene probe of gram positive bacterium and anti-drug. This method of authentication of species and examination of drug-resistant gene consists of these steps: 1. amplify the species gene and anti-drug gene by the primer in the box. 2. Hybridize said two gene probes. This invention of kit has high integrated degree, high sensitivity, and broad application, and high specific activity, stable and reliable detecting signal.

Description

technical field

[0001] The invention relates to a Gram-positive bacterial species identification method and a drug resistance gene detection method and a special kit thereof. Background technique

[0002] 1. Significance of bacterial identification and drug resistance detection

[0003] Bacteria are the pathogens that cause most infectious diseases. The pathogenic bacteria that cause clinical bacterial infections include aerobic bacteria and anaerobic bacteria, which are divided into two categories: Gram-positive bacteria and Gram-negative bacteria. Bacteria and bacilli. Aerobic Gram-positive cocci and Gram-negative bacilli are the most common pathogenic bacteria in clinical practice. Gram-positive bacteria and Gram-negative bacteria have significant differences in their mechanism of action and drug resistance due to the difference in their cell wall and other structures. Clinically, there are great differences in the clinical use of these two types of bacteria.

[0004] ...

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