Tomato deeply processing method
A potato and carbon source technology, applied in the direction of biochemical equipment and methods, applications, microorganisms, etc., can solve the problems of single potato processing methods and low benefits, and achieve the effects of being difficult to imitate, efficient production process, and reducing production costs
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Embodiment 1
[0031] (1) Strain preparation
[0032] Preparation of polyunsaturated oil strains: put the refrigerator-preserved slant strains into the PDA (potato solid medium) slant for activation, cultivate for 7 days, take mycelia and insert them into a 250ml shake flask filled with 100ml PDY (potato liquid) medium Perform liquid culture. The rotation speed is 120-150r / min, after culturing at 25-28°C for 2 days, take the inoculum size (volume fraction v / v) 5-10%, transfer once under the same liquid culture conditions to obtain liquid shake flask species, and then according to the reactor conditions Proceed to seed preparation.
[0033] Preparation of edible and medicinal fungal strains: put the strains stored in the refrigerator on a PDA slope for activation, and cultivate for 7 to 10 days. After the mycelium is full of the slope, take the slope block and put it into a 250ml shaker flask filled with 100ml of PDY medium Perform liquid culture. The rotation speed is 120-150r / min, after ...
Embodiment 2
[0038] Crush the potatoes and add them to water at a ratio of 200g / L, cook them for 20 minutes, filter them with double-layer gauze, add water to adjust the filtrate to the original water volume, and add edible sugar at 50g / L to the filtrate to form a fermentation medium. Add the polyunsaturated oleobacteria seed liquid that embodiment 1 prepares by 5% (v / v), add (NH 4 ) 2 SO 4 0.8g / L, add MnSO in the middle stage of fermentation 4 2.5g / L, add MgSO at the end of fermentation 4 2g / L and other accelerators, fermented for 7 days at a temperature of 25°C and an air flow of 1:1.5, the mycelium biomass was 19.8 / l, the oil content was 6.9g / L, and myristic acid in oil was 0.50%. 15.85% palmitoleic acid, 1.38% palmitoleic acid, 3.30% stearic acid, 37.56% oleic acid, 16.47% linoleic acid, 18.80% gamma-linolenic acid, 0.30% arachidic acid, 0.72% arachidic acid, 0.89 arachidic acid %, EPA 0.80%, DHA 1.98%.
[0039] Produce wet filter residue 120g / 200g potato, add the edible fungus se...
Embodiment 3
[0041] Crush the potatoes and add them to water at a ratio of 200g / L, cook them for 20 minutes, filter them with double-layer gauze, add water to adjust the filtrate to the original water volume, and add edible sugar at 20g / L to the filtrate to form a fermentation medium. According to different periods, that is, adding in the initial stage of fermentation (NH 4 ) 2 SO 4 0.8g / L, add MnSO in the middle stage of fermentation 4 2.5g / L, add MgSO at the end of fermentation 4 Accelerators such as 2g / L, add the polyunsaturated oleaginous bacteria seed liquid that embodiment 1 prepares by 3% (v / v), at 25 ℃ of temperature, after 2 days of fermentation under the condition of aeration volume 1:1.5, according to 30g / L Amount of added edible sugar, followed by fermentation for 5 days, mycelium biomass is 20.5 / l, oil content is 7.2g / L, myristic acid 0.27%, palmitic acid 14.85%, palmitoleic acid 1.38%, hard Fatty acid 3.30%, oleic acid 40.98%, linoleic acid 16.47%, gamma-linolenic acid 18...
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