Polyamide dendroid polymer nano particle loading survivin small molecule RNA

A polyamide branch and nanoparticle technology is applied in the field of new pharmaceutical dosage forms to achieve the effects of increasing intracellular concentration, improving in vitro controllability, and increasing transfection rate

Inactive Publication Date: 2007-08-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recently, polyamide-amine dendrimers (PAMAM dendrimers) used abroad have larger carrying capacity, stronger membrane penetration ability and more sustained-release performance than the previously used polylactic acid-glycolic acid copolymer (PLGA) and other nanocarriers. Good, harmless to human body and many other advantages, but so far there are no reports of polyamide-amine dendrimers carrying survivin-specific small molecule RNA at home and abroad.

Method used

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  • Polyamide dendroid polymer nano particle loading survivin small molecule RNA

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Precisely extract 1 OD small molecule RNA and centrifuge, dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute it with 34 microliters of ultrapure water to obtain solution B ;Take 1 microliter of solution B and dilute it to 15 microliters to obtain solution C; take 1 microliter of solution A and 0.4 microliters of solution C, add it to 18.6 microliters of serum-free medium, and incubate at room temperature for 10 minutes, and then it will survive Prime small molecule RNA polyamide dendrimer nanoparticles.

[0032] The encapsulation of small molecule RNA is shown in Figure 3.

Embodiment 2

[0034] Precisely extract 1 OD small molecule RNA and centrifuge, dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute it with 34 microliters of ultrapure water to obtain solution B ;Take 1 microliter of solution B and dilute it to 15 microliters to obtain solution C; take 1 microliter of solution A and 2 microliters of solution C, add it to 17 microliters of serum-free medium, and incubate at room temperature for 10 minutes, and then it will survive Prime small molecule RNA polyamide dendrimer nanoparticles.

[0035] The encapsulation of small molecule RNA is shown in Figure 3.

Embodiment 3

[0037] Precisely extract 1 OD small molecule RNA and centrifuge, dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute it with 34 microliters of ultrapure water to obtain solution B ;Take 1 microliter of solution B and dilute it to 15 microliters to obtain solution C; take 1 microliter of solution A and 4 microliters of solution C, add it to 15 microliters of serum-free medium, and incubate at room temperature for 10 minutes, and then it will survive Prime small molecule RNA polyamide dendrimer nanoparticles.

[0038] The encapsulation of small molecule RNA is shown in Figure 3.

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Abstract

The invention relates to a method for preparing the carrier RNA polyamide dendric polymer nanometer particles. The invention is characterized in that it uses polyamide dendric polymer as carrier whose amido group number is at 1:10 ratio with the phosphate group number of RNA, to form the polyamide dendric polymer RNA crosslink composite, while the average diameter is 85.64nm at 25Deg. C, with high package on RNA, high carriage, and improved anti-acid and anti-enzyme abilities of RNA, improved cell transfer rate, and the high-efficiency character of small molecule disturbance technique. The invention can be used in the treatment of liver cancer.

Description

technical field [0001] The invention relates to a novel pharmaceutical dosage form, in particular to nano-particles of survivin-loaded small molecule RNA polyamide dendrimer and a preparation method thereof. Background technique [0002] Survivin (Survivin) is an important apoptosis inhibitor newly discovered in recent years. It is selectively expressed in a variety of malignant tumors (especially liver cancer), but not expressed in normal tissue cells. It is possible to inhibit the expression of survivin Promoting the apoptosis of liver cancer cells has become a new target in the current research on the treatment of malignant tumors. [0003] Antisense technology is an effective gene therapy method for tumor treatment research in recent years. It is of great value in blocking the expression of harmful genes and blocking the occurrence and development of tumors. Among various antisense means, the newly emerged ribonucleic acid interference (RNA interference, RNAi) technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K9/14A61K48/00A61P35/00A61P1/16
Inventor 蔡洪培董勤
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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