Unlock instant, AI-driven research and patent intelligence for your innovation.

Method of preparing plant transpiration-resistant agent based on vintage microzyme

A technology of Saccharomyces cerevisiae and anti-transpiration agent, which is applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low adhesion, environmental pollution, poor water solubility, etc., and achieve the effect of Obvious, wide range of application, low cost effect

Inactive Publication Date: 2007-08-15
ZHEJIANG UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two anti-transpiration agents have been widely used in production practice, but they have also exposed a series of problems: such as excessive toxicity, pollution to the environment (such as: mercuric benzene acetate), high cost (ABA), poor water solubility, adhesion Low force (such as: fulvic acid) affects photosynthesis (film type), etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of preparing plant transpiration-resistant agent based on vintage microzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Preparation of bacterial agent A

[0021] (1) Insert the Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain with the preservation number CGMCC2.1 into the sterilized culture medium, place it on a shaker at 28° C., and cultivate it at 100 rpm for 24 hours to obtain the Saccharomyces cerevisiae strain, The content of yeast is detected by drying weighing method, and the content of yeast is 3g / L; the formula of the culture solution is: 5g / L yeast extract, 25g / L glucose, 5g / L ammonium sulfate, 5g / L dihydrogen phosphate Potassium, 4g / L potassium sulfate, 1.5g / L magnesium sulfate, 0.008g / L iron sulfate, 0.008g / L manganese sulfate, prepared with distilled water, adjust pH=6.0;

[0022] (2) Inactivate the above-mentioned Saccharomyces cerevisiae at 121°C and a pressure of 0.12 MPa for 60 minutes. After cooling, centrifuge at 4000 rpm for 40 minutes. The obtained yeast extract precipitate is dehydrated with 100% ethanol and placed in - Freeze at 20°C to prepare bacter...

Embodiment 2

[0028] 1) Preparation of bacterial agent A

[0029] (1) Insert the Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain with the preservation number CGMCC2.1 into the sterilized culture medium, place it on a shaker at 30°C, and cultivate it at 150 rpm for 30 hours to obtain the Saccharomyces cerevisiae bacterial liquid, The content of yeast is detected by drying weighing method, and the content of yeast is 5g / L; the formula of the culture solution is: 5g / L yeast extract, 25g / L glucose, 5g / L ammonium sulfate, 5g / L dihydrogen phosphate Potassium, 4g / L potassium sulfate, 1.5g / L magnesium sulfate, 0.008g / L iron sulfate, 0.008g / L manganese sulfate, prepared with distilled water, adjust pH=6.0;

[0030] (2) Inactivate the above-mentioned Saccharomyces cerevisiae at 121° C. under a pressure of 0.15 MPa for 90 minutes. After cooling, centrifuge at 6000 rpm for 60 minutes. The obtained yeast extract precipitate is dehydrated with 100% ethanol and placed in - Freeze at 20°C to pr...

Embodiment 3

[0036] 1) Preparation of bacterial agent A

[0037] (1) Insert the Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain with the preservation number CGMCC2.1 into the sterilized culture medium, place it on a shaker at 29° C., and cultivate it at 130 rpm for 28 hours to obtain the Saccharomyces cerevisiae bacterial liquid, The content of yeast is detected by drying weighing method, and the content of yeast is 4g / L; the formula of the culture solution is: 5g / L yeast extract, 25g / L glucose, 5g / L ammonium sulfate, 5g / L dihydrogen phosphate Potassium, 4g / L potassium sulfate, 1.5g / L magnesium sulfate, 0.008g / L iron sulfate, 0.008g / L manganese sulfate, prepared with distilled water, adjust pH=6.0;

[0038] (2) Inactivate the above Saccharomyces cerevisiae bacteria liquid at 121°C and 0.13 MPa pressure for 80 minutes, after cooling, centrifuge at 5000 rpm for 50 minutes, extract the precipitate from the yeast, dehydrate it with 100% ethanol, and put it in - Freeze at 20°C to pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparing method of plant anti-transpiration agent based on saccharomyces cerevisiae bacteria, which comprises the following steps: 1) preparing bacterium agent A; (1) switching saccharomyces cerevisiae bacteria strain into biocidal culture liquid; placing on shaker; culturing; getting saccharomyces cerevisiae bacteria liquid; testing the content of yeast with dried weight method; (2) deactivating bacteria liquid under 0.12-0.15 MP at 121 deg.c; cooling; centrifugal separating; getting the yeast to extract deposition; dewatering with 100% alcohol; storing at -20 deg.c; producing bacterium agent A; 2) preparing aids B; allocating aids with 2.0g / L CaCl2 and 0.01g / L deacetylate chitin and sterilized water; 3) preparing anti transpiration agent; adding bacterium agent A into aids b; stirring evenly; getting the produce. This invention possesses merits of safe, environmental protection and cheap cost, which belongs to a new generation biological anti-drying agent.

Description

technical field [0001] The invention relates to a preparation method of a plant anti-transpiration agent based on Saccharomyces cerevisiae. Background technique [0002] With the impact of global warming and human activities, water resources have gradually become one of the main factors restricting economic and social development. As the basic industry of the national economy, agriculture has always been a major user of water. In my country, due to the uneven distribution of water resources in time and space and the backwardness of basic water conservancy facilities, the water utilization rate of agricultural irrigation is only about 40%, which is much lower than that of the United States, Israel and other western developed countries. Therefore, the development of new plant water-saving and drought-resistant agents to improve the water use efficiency of crop vegetation is an indispensable part of the development of water-saving agriculture in my country. So far, there are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/04C12R1/865
Inventor 王根轩甘毅邱木清康燕
Owner ZHEJIANG UNIV