Method for preparing heterotrophic ammonia-oxidizing bacterium and use thereof

An ammonia oxidizing inoculant and ammonia oxidation technology are applied in the field of preparation of environmental microbial inoculants, which can solve the problems of competition of heterotrophic bacteria, low nitrification efficiency, environmental impact, etc., achieve resistance to high COD/N load, improve preservation methods, The effect of reducing infrastructure investment

Inactive Publication Date: 2007-08-29
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional biological denitrification process, the growth rate of nitrifying bacteria is slow, they cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, it is difficult to obtain high biomass, a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] One, the preparation of heterotrophic ammonia oxidizing bacteria agent

[0036] 1. Quantify the LB solid medium that has been subjected to high-temperature sterilization (121°C, 25 minutes) for future use. The formula for the LB solid medium is (weight / volume): 1% peptone, 0.5% yeast extract, 1% NaCl, and 100 mL of water , pH7.0, agar 1.5%.

[0037] 2. Inoculate strains H9, H10, and H13 into sterilized (121° C., 25 minutes) LB solid slant medium to activate for later use.

[0038] 3. Quantify the liquid medium that has been subjected to high-temperature sterilization (121°C, 25 minutes) for subsequent use, and the formula of the liquid medium is (NH 4 ) SO 4 1.0g; NaCl 20g; K 2 HPO 4 0.75g; NaHPO 4 0.25g; MgSO 4 0.03g; FeSO 4 .7H 2 O 0.03g; MnSO 4 0.01g; Na 2 CO 3 0.5g; peptone 2g; yeast extract 1g; glucose 5g; water 1000mL, pH7.5.

[0039] 4. Insert the activated strains into the liquid medium for aeration and aerobic culture for 48 hours.

[0040] 5...

example 1

[0043] Example 1: Activate 50 grams of solid heterotrophic ammonia oxidizing bacteria agent and add it to the SBR reactor with 5L COD / N=8, BOD / COD≈0.45 ammonia-nitrogen-containing wastewater, and add nitrification sludge; at the same time, only The control with nitrification sludge and the blank control without nitrification sludge and bacterial agent. After 48 hours of uniform treatment, samples were taken to determine the content of ammonia nitrogen in the wastewater, and the results are as follows:

[0044] Table 1: Comparison of the treatment effect of ammonia nitrogen wastewater with and without bacterial agent

[0045] category

[0046]It can be seen from the above table that when no bacterial agent is added, the removal rate of ammonia nitrogen in high COD / N wastewater by nitrification sludge is only 30.78%. After adding bacterial agent, the removal rate of ammonia nitrogen reaches 56.82%. The removal rate of fungicide increased by 26.04%.

example 2

[0047] Example 2: Add 1ml of liquid heterotrophic ammonia oxidizing bacteria agent to a conical flask containing 100mL of sterilized inorganic nitrosation medium, shake and cultivate at 30°C and 150 rpm for 48 hours, and add only nitrification sludge at the same time The control and the blank control without nitrification sludge and bacterial agent. The processing results are shown in the table below.

[0048] Table 2: The treatment effect of adding bacteria agent and not adding bacteria agent to nitrosating medium

[0049] category

[0050] It can be seen from the above table that the removal rate of ammonia nitrogen by nitrification sludge is 60.81%, and the removal rate of ammonia nitrogen by bacterial agent is 68.75%, which shows that the bacterial agent can also effectively nitrify ammonia nitrogen in inorganic ammonia nitrogen medium, and its nitrification effect higher than that of nitrified sludge.

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Abstract

The invention relates to a making method and application for environment microorganism fungus agent to process ammonia nitrogen in waste water. It is realized by mixing heterotrophy ammoxidation fungus H9, H10, and H13 according to 3:1:1. The fungus agent has the advantages of high nitrating efficiency, relieving restraining effect of nitrating by high COD/N.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a preparation method and application of an environmental microbial agent for treating ammonia nitrogen in wastewater. Background technique [0002] Biological denitrification is currently recognized as one of the most economical and effective methods for denitrification of wastewater. The fundamental way to remove ammonia nitrogen pollution is to convert nitrate nitrogen into nitrate nitrogen through nitrification reaction, and then convert nitrate nitrogen into gaseous nitrogen through denitrification and discharge it into the atmosphere, so as to realize the cycle of nitrogen element and the harmlessness of ammonia nitrogen. The traditional biological denitrification process mainly includes two stages of nitrification and denitrification, which are completed by nitrifying bacteria and denitrifying bacteria respectively. This process is currently the most important p...

Claims

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Application Information

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IPC IPC(8): C12N1/00
Inventor 李旭东闫志英廖银章谭周亮
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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