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Magnetic nano cell automatic sorting device and uses thereof

A magnetic nano, automatic sorting technology, applied in the field of biomedicine, can solve the problems that the sorting system cannot realize automatic sorting, and achieve the effect of reducing the occurrence of GVHD, reducing the occurrence of GVHD, and reducing the cost of separation

Inactive Publication Date: 2007-09-26
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a magnetic nano-cell automatic sorting device and its application to overcome the inability of the existing sorting system to realize automation and the sorting of a large number of cells

Method used

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  • Magnetic nano cell automatic sorting device and uses thereof
  • Magnetic nano cell automatic sorting device and uses thereof
  • Magnetic nano cell automatic sorting device and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Synthesis of magnetic nanoparticles

[0042] FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 The mixed solution of O and NaOH solution adopts co-precipitation method to form γ-Fe 2 O 3 Nanoparticles, and then use the reverse microemulsion method to hydrolyze the ethyl orthosilicate while enveloping the chitosan in the γ-Fe 2 O 3 On the outside, core-shell type magnetic nanoparticles are formed and characterized by TEM and Mössbauer spectrometer.

Embodiment 2

[0044] Anti-CD34 monoclonal antibody attached to magnetic nanoparticles

[0045] Disperse a certain amount of the above-mentioned amino-linked magnetic nanoparticles in phosphate buffer (Phosphatebuffered saline, PBS, 0.1M, pH7.4), add a certain amount of glutaraldehyde, and after reacting for 4-5 hours, remove the excess pentane Wash off the dialdehyde, redisperse it in PBS, then add a certain amount of anti-CD34 monoclonal antibody, react for 1.5-2.5 hours, then wash off the excess antibody with PBS, and collect the remaining liquid (antibody reacts with magnetic nanoparticles) The remaining liquid afterwards), two rinsing liquids (referred to as one wash and two washes respectively).

Embodiment 3

[0047] Morphology of isolated CD34+ cells

[0048] Figure 2 is a SEM photo of CD34+ cells with magnetic nanoparticles. With the help of SEM, it can be seen that the separated CD34+ cells still maintain their normal morphology, and at the same time, we can also see the magnetic nanoparticles attached to the cell surface. From the figure, we can also see that the particle size of magnetic nanoparticles is small relative to cells. Although magnetic nanoparticles have a certain agglomeration phenomenon in complex biological systems, they do not affect the separation effect of cells and do not affect the morphology of cells too much. Impact. Therefore, the use of magnetic nanoparticles to separate CD34+ cells has little effect on the morphology of the cells.

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Abstract

The invention discloses a magnetic behavior nanometer cell automatic sorting device and appliance, which is characterized by the following: supplying magnetic behavior sorting system, example transferring system and computer controlling system; setting the magnetic sorting system at least with magnet sorting solution and molecule; using to separating candidate stem cell from peripheral blood or navel cord blood; adopting micro-computer and modern controlling technology; automatic-controlling for cell sorting and washing course of the whole course. This invention can be used as cell source for cytology research, medicine screening and animal experiment.

Description

Technical field [0001] The invention belongs to the field of biomedical technology, and particularly relates to a device for cell sorting using magnetic nanoparticles. Background technique [0002] Since the 1980s, hematopoietic stem cell transplantation has become the main measure of hematopoietic support treatment after high-dose radiotherapy and chemotherapy for malignant tumors (such as some malignant hematological diseases and solid tumors of non-hematological system, etc.). With the support of hematopoietic stem cell transplantation, clinicians can use ultra-large doses of radiotherapy and chemotherapy to eliminate cancer cells in the patient to the greatest extent, and then implant hematopoietic stem cells to rebuild the damaged hematopoietic and immune system, and improve the risk of malignant tumors. Treatment effect and patient's survival period. Since adult hematopoietic stem cells mainly exist in the bone marrow, hematopoietic stem cells derived from bone marrow are i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/08
Inventor 沈鹤柏潘建国董亚明朱龙章韩林峰
Owner SHANGHAI NORMAL UNIVERSITY
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